Results 221 to 230 of about 574,812 (244)

Real-Time RT-PCR: cDNA Synthesis

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol uses the Superscript II First-Strand Synthesis System for the generation of cDNA from total RNA.
Wolfgang, Kusser, Sandrine, Javorschi, Martin A, Gleeson   +2 more
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Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
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Quantification of mRNA using real-time RT-PCR

Nature Protocols, 2006
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA.
Tania, Nolan, Rebecca E, Hands, Stephen A, Bustin   +2 more
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Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
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RNA Isolation and Real-Time Quantitative RT-PCR

2008
Adipose tissue has emerged as a major endocrine organ producing a wide spectrum of hormones and factors that play crucial roles in regulating cell turnover and function, not only locally within the adipose tissue but also in the brain and other key metabolic organ systems.
Haiyan, Guan, Kaiping, Yang
openaire   +2 more sources

Quantitative (Real-Time) RT-PCR in Cardiovascular Research

2007
Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples.
Ashton, KJ, Headrick, JP
openaire   +2 more sources

Identification of nasal blood by real-time RT-PCR

Legal Medicine, 2012
A new approach for the identification of body fluid stains by comparing specific mRNA expression levels has been extensively studied in recent years. Here, we examine whether nasal blood, which is regarded as one of the most difficult types of blood to identify, can be identified by comparing mRNA expression levels of target genes specific to saliva ...
Koichi, Sakurada, Tomoko, Akutsu, Ken, Watanabe, Mineo, Yoshino   +3 more
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Real-time RT-PCR for norovirus screening in shellfish

Journal of Virological Methods, 2005
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II.
Loisy, F, Atmar, RL, Guillon, P, Le Cann, P, Pommepuy, M, Le Guyader, FS   +5 more
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Monitoring Gene Expression: Quantitative Real-Time RT-PCR

2013
Two-step quantitative real-time RT-PCR (RT-qPCR), also known as real-time RT-PCR, kinetic RT-PCR, or quantitative fluorescent RT-PCR, has become the method of choice for gene expression analysis during the last few years. It is a fast and convenient PCR method that combines traditional RT-PCR with the phenomenon of fluorescence resonance energy ...
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Quantification of GPCR mRNA Using Real-Time RT-PCR

2011
Characterisation of G-protein-coupled receptor (GPCR) mRNA expression under normal, different pharmacological and pathological conditions in experimental animal models and human tissue biopsies by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a valuable approach to understand the regulation of GPCR expression.
Trond, Brattelid, Finn Olav, Levy
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