Results 251 to 260 of about 637,318 (299)

Genetic Characterization of Palyam Serogroup Viruses Isolated in Japan from 1984 to 2018 and Development of a Real-Time RT-PCR Assay for Broad Detection of Palyam Serogroup Viruses and Specific Detection of Chuzan (Kasba) and D'Aguilar Viruses. [PDF]

Pathogens
Shirafuji H, Kishida N, Murota K, Suda Y, Yanase T.   +4 more
europepmc   +1 more source

Some of the next articles are maybe not open access.

Related searches:

Stepwise Optimization of Real-Time RT-PCR Analysis

2023
Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR parameters are critical for the accuracy and reproducibility of qRT-PCR analysis.
Nathan A, Maren, James R, Duduit, Debao, Huang, Fanghou, Zhao, Thomas G, Ranney, Wusheng, Liu   +5 more
openaire   +2 more sources

Real-Time RT-PCR: cDNA Synthesis

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol uses the Superscript II First-Strand Synthesis System for the generation of cDNA from total RNA.
Wolfgang, Kusser, Sandrine, Javorschi, Martin A, Gleeson   +2 more
openaire   +2 more sources

Quantification of mRNA using real-time RT-PCR

Nature Protocols, 2006
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA.
Tania, Nolan, Rebecca E, Hands, Stephen A, Bustin   +2 more
openaire   +2 more sources

Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
openaire   +2 more sources

Identification of nasal blood by real-time RT-PCR

Legal Medicine, 2012
A new approach for the identification of body fluid stains by comparing specific mRNA expression levels has been extensively studied in recent years. Here, we examine whether nasal blood, which is regarded as one of the most difficult types of blood to identify, can be identified by comparing mRNA expression levels of target genes specific to saliva ...
Koichi, Sakurada, Tomoko, Akutsu, Ken, Watanabe, Mineo, Yoshino   +3 more
openaire   +2 more sources

Real-time RT-PCR for norovirus screening in shellfish

Journal of Virological Methods, 2005
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II.
Loisy, F, Atmar, RL, Guillon, P, Le Cann, P, Pommepuy, M, Le Guyader, FS   +5 more
openaire   +3 more sources

Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
openaire   +2 more sources

RNA Isolation and Real-Time Quantitative RT-PCR

2008
Adipose tissue has emerged as a major endocrine organ producing a wide spectrum of hormones and factors that play crucial roles in regulating cell turnover and function, not only locally within the adipose tissue but also in the brain and other key metabolic organ systems.
Haiyan, Guan, Kaiping, Yang
openaire   +2 more sources