Results 261 to 270 of about 637,318 (299)
Some of the next articles are maybe not open access.
Quantitative (Real-Time) RT-PCR in Cardiovascular Research
2007Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples.
Ashton, KJ, Headrick, JP
openaire +2 more sources
Quantitation of ovine cytokine mRNA by real-time RT–PCR
Journal of Immunological Methods, 2006In this study we describe for the first time the dynamics of the expression of the cytokines, IL-1beta, IL-12p40, TNFalpha in ovine dendritic cells and macrophages after LPS stimulation. Real time RT-PCR was used for the quantitation of these cytokines and IL-4 and IFNgamma as well as two potential housekeeping genes (HKG), ATPase and GAPDH, in mRNAs ...
Sangeeta, Budhia +3 more
openaire +2 more sources
One-step real time RT-PCR for detection of microRNAs
Talanta, 2013Rapid and simple methods for microRNA (miRNA) detection are essential for biological research of miRNAs and clinical diagnosis. Here we describe a sensitive and specific real time RT-PCR (also RT-qPCR) method for miRNA quantification. The whole detection process including reverse transcription and PCR is performed in one PCR tube by a one-step ...
Jingli, Yan +4 more
openaire +2 more sources
A Real-Time RT-PCR Method for the Universal Detection and Identification of Flaviviruses
Vector-Borne and Zoonotic Diseases, 2007(Uploaded by Plazi for the Bat Literature Project) Here we describe an optimized molecular protocol for the universal detection and identification of flaviviruses. It combines the convenient real-time polymerase chain reaction (PCR) format with a broad spectrum of flavivirus detection.
G, Moureau +5 more
openaire +2 more sources
Quantification of GPCR mRNA Using Real-Time RT-PCR
2011Characterisation of G-protein-coupled receptor (GPCR) mRNA expression under normal, different pharmacological and pathological conditions in experimental animal models and human tissue biopsies by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a valuable approach to understand the regulation of GPCR expression.
Trond, Brattelid, Finn Olav, Levy
openaire +2 more sources
Real-Time RT-PCR for CTG Repeat-Containing Genes
2004Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion in the 3'-untranslated region (UTR) of myotonic dystrophy protein kinase (DMPK). SIX5 is a homeodomain gene located just downstream of the repeat, and myotonic dystrophy WD protein (DMWD) is located close upstream of DMPK.
openaire +2 more sources
Monitoring Gene Expression: Quantitative Real-Time RT-PCR
2013Two-step quantitative real-time RT-PCR (RT-qPCR), also known as real-time RT-PCR, kinetic RT-PCR, or quantitative fluorescent RT-PCR, has become the method of choice for gene expression analysis during the last few years. It is a fast and convenient PCR method that combines traditional RT-PCR with the phenomenon of fluorescence resonance energy ...
openaire +2 more sources
Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR
2014Real-time RT-PCR (rRT-PCR) has been used for avian influenza virus (AIV) detection since the early 2000s. This method has been applied to surveillance, outbreaks and research. Some of the advantages of rRT-PCR are high sensitivity, high specificity, rapid time to result, scalability, cost, and its inherently quantitative nature.
openaire +3 more sources
REAL-TIME (RT-PCR) DETECTION OF MONKEYPOX VİRUS
2022Polimeraz Zincir Reaksiyonu (PCR), küçük DNA fragmanlarında belirli dizilerin hızlandırılmış amplifikasyonuna izin veren in vitro replikasyonudur. Hassas bir teknik olan PCR’nin, analiz edilecek kadar kopya üretmesi için yalnızca DNA izlerine ihtiyaç vardır.
TAHİROĞLU, Veysel +2 more
openaire +1 more source
Detection and differentiation of Avian Metapneumovirus by Real Time Rt-PCR
2012Direct diagnosis of avian Metapneumovirus (AMPV) infections rely on molecular techniques more than on virus isolation due to the fastidious nature of the virus. Six real-time reverse transcription PCR (RRT-PCR) protocols for the detection and differentiation of AMPV subtype A and B were developed in N (two tests), F, SH (two tests) and G genes.
Cecchinato M. +5 more
openaire +2 more sources