Results 231 to 240 of about 625,783 (265)

Quantification of GPCR mRNA Using Real-Time RT-PCR

2011
Characterisation of G-protein-coupled receptor (GPCR) mRNA expression under normal, different pharmacological and pathological conditions in experimental animal models and human tissue biopsies by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a valuable approach to understand the regulation of GPCR expression.
Trond, Brattelid, Finn Olav, Levy
openaire   +2 more sources

Real-Time RT-PCR for CTG Repeat-Containing Genes

2004
Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion in the 3'-untranslated region (UTR) of myotonic dystrophy protein kinase (DMPK). SIX5 is a homeodomain gene located just downstream of the repeat, and myotonic dystrophy WD protein (DMWD) is located close upstream of DMPK.
openaire   +2 more sources

Monitoring Gene Expression: Quantitative Real-Time RT-PCR

2013
Two-step quantitative real-time RT-PCR (RT-qPCR), also known as real-time RT-PCR, kinetic RT-PCR, or quantitative fluorescent RT-PCR, has become the method of choice for gene expression analysis during the last few years. It is a fast and convenient PCR method that combines traditional RT-PCR with the phenomenon of fluorescence resonance energy ...
openaire   +2 more sources

Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR

2014
Real-time RT-PCR (rRT-PCR) has been used for avian influenza virus (AIV) detection since the early 2000s. This method has been applied to surveillance, outbreaks and research. Some of the advantages of rRT-PCR are high sensitivity, high specificity, rapid time to result, scalability, cost, and its inherently quantitative nature.
openaire   +3 more sources

REAL-TIME (RT-PCR) DETECTION OF MONKEYPOX VİRUS

2022
Polimeraz Zincir Reaksiyonu (PCR), küçük DNA fragmanlarında belirli dizilerin hızlandırılmış amplifikasyonuna izin veren in vitro replikasyonudur. Hassas bir teknik olan PCR’nin, analiz edilecek kadar kopya üretmesi için yalnızca DNA izlerine ihtiyaç vardır.
TAHİROĞLU, Veysel, ALAYUNT, Naci Ömer, ÖZTÜRK, Cihat   +2 more
openaire   +1 more source

Detection and differentiation of Avian Metapneumovirus by Real Time Rt-PCR

2012
Direct diagnosis of avian Metapneumovirus (AMPV) infections rely on molecular techniques more than on virus isolation due to the fastidious nature of the virus. Six real-time reverse transcription PCR (RRT-PCR) protocols for the detection and differentiation of AMPV subtype A and B were developed in N (two tests), F, SH (two tests) and G genes.
Cecchinato M., LUPINI, CATERINA, Mondin A., Munoz Pogoreltseva O. S., LISTORTI, VALERIA, CATELLI, ELENA   +5 more
openaire   +2 more sources

[Real-time polymerase chain reaction (RT-PCR)].

Annales Academiae Medicae Stetinensis, 2008
The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology,
Katarzyna, Wiedro, Ewa, Stachowska, Dariusz, Chlubek   +2 more
openaire   +1 more source

Real-Time Quantitative RT-PCR Assays

2003
Martin I, Frank O
openaire   +3 more sources

Real-Time RT-PCR for Detection of Feline Coronavirus

2022
Leyi Wang, Colleen Olmstead, Richard Fredrickson   +2 more
openaire   +1 more source

Quantification using real-time PCR technology: applications and limitations

Trends in Molecular Medicine, 2002
Dieter Klein
exaly