Results 251 to 260 of about 18,885,351 (305)

Broad-spectrum dengue virus detection using the commercial RealStar dengue RT-PCR kit 3.0 (Altona) and an in-house combined real-time RT-PCR assay. [PDF]

Heliyon
Luciani L, Combe P, Touret F, Gazin C, Klitting R, Pezzi L, Thirion L, Charrel R, Grard G, de Lamballerie X, Nougairède A.   +10 more
europepmc   +1 more source

Adipocyte Myoglobin Is a Determinant of Energy Expenditure and a Potential Target to Limit Obesity

Advanced Science, EarlyView.
Myoglobin, known as a muscle oxygen‐carrying protein, is shown to play a key role in fat cells that burn energy. Loss of myoglobin reduces the body's ability to generate heat and increases obesity risk, while restoring it improves metabolism. The study identifies myoglobin as a regulator of fat burning and a potential target to enhance energy ...
Christian Strehlau, Helen Broghammer, Claudia Gebhardt, Anne Hoffmann, Tobias Hagemann, Şeyma Midilli, Rachel S. Zimmer, Kristin Schubert, Vasiliki Karagiannakou, Anastasia Georgiadi, Mario Ost, Martin Krüger, Lisa Roth, Kerstin Krause, Nora Klöting, Maria Keller, Martin Wabitsch, Rima Nuwayhid, Roland H. Stimson, Michael Stumvoll, Matthias Blüher, Juliane Weiner, John T. Heiker   +22 more
wiley   +1 more source

Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. [PDF]

Viruses
Villalba R, Tena-Tomás C, Ruano MJ, Valero-Lorenzo M, López-Herranz A, Cano-Gómez C, Agüero M.   +6 more
europepmc   +1 more source

Rapid Differential Detection of Wild-Type Classical Swine Fever Virus and Hog Cholera Lapinized Virus Vaccines by TaqMan MGB-Based Dual One-Step Real-Time RT-PCR. [PDF]

Vet Sci
Zhang Y, Wang M, Sun Y, Xiao X, Wang S, Li P, Liu Y, Zhao H, Meng Y, Yin R.   +9 more
europepmc   +1 more source

Genetic Characterization of Palyam Serogroup Viruses Isolated in Japan from 1984 to 2018 and Development of a Real-Time RT-PCR Assay for Broad Detection of Palyam Serogroup Viruses and Specific Detection of Chuzan (Kasba) and D'Aguilar Viruses. [PDF]

Pathogens
Shirafuji H, Kishida N, Murota K, Suda Y, Yanase T.   +4 more
europepmc   +1 more source

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Stepwise Optimization of Real-Time RT-PCR Analysis

2023
Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR parameters are critical for the accuracy and reproducibility of qRT-PCR analysis.
Nathan A, Maren, James R, Duduit, Debao, Huang, Fanghou, Zhao, Thomas G, Ranney, Wusheng, Liu   +5 more
openaire   +2 more sources

Real-Time RT-PCR: cDNA Synthesis

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol uses the Superscript II First-Strand Synthesis System for the generation of cDNA from total RNA.
Wolfgang, Kusser, Sandrine, Javorschi, Martin A, Gleeson   +2 more
openaire   +2 more sources

Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
openaire   +2 more sources

Identification of nasal blood by real-time RT-PCR

Legal Medicine, 2012
A new approach for the identification of body fluid stains by comparing specific mRNA expression levels has been extensively studied in recent years. Here, we examine whether nasal blood, which is regarded as one of the most difficult types of blood to identify, can be identified by comparing mRNA expression levels of target genes specific to saliva ...
Koichi, Sakurada, Tomoko, Akutsu, Ken, Watanabe, Mineo, Yoshino   +3 more
openaire   +2 more sources