Results 261 to 270 of about 18,885,351 (305)

Quantification of mRNA using real-time RT-PCR

Nature Protocols, 2006
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA.
Tania, Nolan, Rebecca E, Hands, Stephen A, Bustin   +2 more
openaire   +2 more sources

Real-time RT-PCR for norovirus screening in shellfish

Journal of Virological Methods, 2005
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II.
Loisy, F, Atmar, RL, Guillon, P, Le Cann, P, Pommepuy, M, Le Guyader, FS   +5 more
openaire   +3 more sources

Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
openaire   +2 more sources

RNA Isolation and Real-Time Quantitative RT-PCR

2008
Adipose tissue has emerged as a major endocrine organ producing a wide spectrum of hormones and factors that play crucial roles in regulating cell turnover and function, not only locally within the adipose tissue but also in the brain and other key metabolic organ systems.
Haiyan, Guan, Kaiping, Yang
openaire   +2 more sources

Quantitative (Real-Time) RT-PCR in Cardiovascular Research

2007
Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples.
Ashton, KJ, Headrick, JP
openaire   +2 more sources

Quantitation of ovine cytokine mRNA by real-time RT–PCR

Journal of Immunological Methods, 2006
In this study we describe for the first time the dynamics of the expression of the cytokines, IL-1beta, IL-12p40, TNFalpha in ovine dendritic cells and macrophages after LPS stimulation. Real time RT-PCR was used for the quantitation of these cytokines and IL-4 and IFNgamma as well as two potential housekeeping genes (HKG), ATPase and GAPDH, in mRNAs ...
Sangeeta, Budhia, Lisa F, Haring, Ian, McConnell, Barbara A, Blacklaws   +3 more
openaire   +2 more sources

One-step real time RT-PCR for detection of microRNAs

Talanta, 2013
Rapid and simple methods for microRNA (miRNA) detection are essential for biological research of miRNAs and clinical diagnosis. Here we describe a sensitive and specific real time RT-PCR (also RT-qPCR) method for miRNA quantification. The whole detection process including reverse transcription and PCR is performed in one PCR tube by a one-step ...
Jingli, Yan, Nan, Zhang, Cui, Qi, Xiangjun, Liu, Dihua, Shangguan   +4 more
openaire   +2 more sources

A Real-Time RT-PCR Method for the Universal Detection and Identification of Flaviviruses

Vector-Borne and Zoonotic Diseases, 2007
(Uploaded by Plazi for the Bat Literature Project) Here we describe an optimized molecular protocol for the universal detection and identification of flaviviruses. It combines the convenient real-time polymerase chain reaction (PCR) format with a broad spectrum of flavivirus detection.
G, Moureau, S, Temmam, J P, Gonzalez, R N, Charrel, G, Grard, X, de Lamballerie   +5 more
openaire   +2 more sources

Quantification of GPCR mRNA Using Real-Time RT-PCR

2011
Characterisation of G-protein-coupled receptor (GPCR) mRNA expression under normal, different pharmacological and pathological conditions in experimental animal models and human tissue biopsies by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a valuable approach to understand the regulation of GPCR expression.
Trond, Brattelid, Finn Olav, Levy
openaire   +2 more sources

Real-Time RT-PCR for CTG Repeat-Containing Genes

2004
Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion in the 3'-untranslated region (UTR) of myotonic dystrophy protein kinase (DMPK). SIX5 is a homeodomain gene located just downstream of the repeat, and myotonic dystrophy WD protein (DMWD) is located close upstream of DMPK.
openaire   +2 more sources