Realtime PCR is more sensitive than multiplex PCR for diagnosis and serotyping in children with culture negative pneumococcal invasive disease. [PDF]
BACKGROUND: Pneumococcal serotyping is usually performed by Quellung reaction, considered the gold standard test. However the method cannot be used on culture-negative samples. Molecular methods can be a useful alternative.
Chiara Azzari +8 more
doaj +4 more sources
Performance evaluation of the Alinity m system for quantifying cytomegalovirus DNA in samples of the respiratory, gastrointestinal, and urinary tract [PDF]
Quantitation of cytomegalovirus (CMV) DNA load in specimens other than blood such as bronchoalveolar lavages, intestinal biopsies, or urine has become a common practice as an ancillary tool for the diagnosis of CMV pneumonitis, intestinal disease, or ...
Eliseo Albert +7 more
doaj +2 more sources
Optimize realtime PCR reaction to detect Streptococcus agalactiae
Streptococcus agalactiae (GBS) is the major contagious cause of early-onset sepsis in newborns. Screening and intrapartum antibiotic prophylaxis for GBS in pregnant women could effectively prevent early-onset GBS infection in newborns.
Nguyễn Thị Thanh Thảo +3 more
doaj +1 more source
Indonesia is a country where the majority of the population is seasonal, so the halalness of a product is very important. Supplements are one of the products that are widely circulated in Indonesia, so they must provide halal guarantees for these ...
Firman Rezaldi +3 more
doaj +1 more source
METHODOLOGICAL ASPECTS OF REAL-TIME PCR USAGE IN ACETOBACTER DETECTION [PDF]
Acetic acid bacteria occur in sugar and alcoholised, slightly acid niches such as flowers, fruits, beer, wine, cider, vinegar, souring fruit juices and honey.
BOISTEAN, Alina +4 more
doaj +1 more source
TULP4, a novel E3 ligase gene, participates in neuronal migration as a candidate in schizophrenia
Mutations identified from four SCZ pedigrees resulted in decreased TULP4 expression. Tulp4 knockdown caused delayed neuron migration in embryonic mice, and impaired cognition and prepulse inhibition in adult mice. These phenotypes may be related to TULP4 through its involvement in the formation of a novel E3 ubiquitin ligases.
Yan Bi +19 more
wiley +1 more source
Diagnostic Efficiency of Different Serological Tests and Real time PCR for Detecting Brucella Infection in Camels' Sera [PDF]
px; "> complement fixation test (CFT) was done on 335 camels sera. Real-time PCR,classified 335 camel serum samples to 268 (80%) as positive and 67 (20%) asnegative.
Mahmoud E.R. Hamdy +3 more
doaj +1 more source
Relevance. The use of gene therapy drugs for the treatment of genetic diseases and stimulation of regeneration processes is lengthy and involves repeated injections, which may lead to increased dissemination of gene therapy constructs from the injection ...
S. S. Dzhauari +5 more
doaj +1 more source
Objectives: To evaluate the performance of the newly developed technology Abbott RealTime MTB assay (RealTime MTB assay) for the detection of Mycobacterium tuberculosis in sputum specimens and to compare its performance with that of the Cepheid GeneXpert
Sheng Fen Wang +5 more
doaj +1 more source
Application of multiplex realtime PCR detection for hemorrhagic fever syndrome viruses
Background: Multiplex real-time PCR is a quick and cost effective method for detection of various gene simultaneously. HFSV (Hemorrhagic Fever Syndrome Virus) is a newly emerging infectious disease because of globalization and climate change. We tried to
Yoonhyuk Choi, Younghee Kim
doaj +1 more source

