Results 11 to 20 of about 32,209 (206)

Realtime PCR is more sensitive than multiplex PCR for diagnosis and serotyping in children with culture negative pneumococcal invasive disease. [PDF]

PLoS ONE, 2010
BACKGROUND: Pneumococcal serotyping is usually performed by Quellung reaction, considered the gold standard test. However the method cannot be used on culture-negative samples. Molecular methods can be a useful alternative.
Chiara Azzari, Maria Moriondo, Giuseppe Indolfi, Martina Cortimiglia, Clementina Canessa, Laura Becciolini, Francesca Lippi, Maurizio de Martino, Massimo Resti   +8 more
doaj   +8 more sources

Optimize realtime PCR reaction to detect Streptococcus agalactiae

Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2021
Streptococcus agalactiae (GBS) is the major contagious cause of early-onset sepsis in newborns. Screening and intrapartum antibiotic prophylaxis for GBS in pregnant women could effectively prevent early-onset GBS infection in newborns.
Nguyễn Thị Thanh Thảo, Nguyễn Thị Trúc Phương, Nguyễn Thị Trúc Anh, Lương Thị Mỹ Ngân   +3 more
doaj   +4 more sources

Application of multiplex realtime PCR detection for hemorrhagic fever syndrome viruses

Journal of Infection and Public Health, 2023
Background: Multiplex real-time PCR is a quick and cost effective method for detection of various gene simultaneously. HFSV (Hemorrhagic Fever Syndrome Virus) is a newly emerging infectious disease because of globalization and climate change. We tried to
Yoonhyuk Choi, Younghee Kim
doaj   +3 more sources

Detection of bovine herpesvirus 4 DNA in cattle by realtime PCR

Вопросы вирусологии, 2019
Introduction. BoHV-4 is poorly understood. Data on the circulation of the virus among animals and its role in infectious diseases insufficient. Aimes and goals.
A. V. Nefedchenko, A. G. Yuzhakov, S. V. Koteneva, T. I. Glotova, A. G. Glotov, A. D. Zaberezhny   +5 more
doaj   +4 more sources

Comparison of commercial realtime reverse transcription PCR assays for the detection of SARS-CoV-2

Journal of Clinical Virology, 2020
The emergence of a new coronavirus in Wuhan China has triggered a global need for accurate diagnostic assays. Initially, mostly laboratory developed molecular tests were available but shortly thereafter different commercial assays started to appear and are still increasing in number.
Zsofia Igloi, Veerle Matheeussen, Marion P G Koopmans   +2 more
exaly   +4 more sources

Establisment of realtime pcr protocol based on 16s rrna gene of mycobacterium tuberculosis

Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2011
Currently, tuberculosis is still one of the most common infectious diseases, mainly caused by Mycobacterium tuberculosis bacterium infection. PCR is a molecular biology technique used to detect infectious agents, especially viruses or bacteria with ...
Lê Huyền Ái Thúy, Trương Kim Phượng, Hồ Thị Thanh Thủy   +2 more
doaj   +1 more source

Performance evaluation of the Alinity m system for quantifying cytomegalovirus DNA in samples of the respiratory, gastrointestinal, and urinary tract [PDF]

Microbiology Spectrum
Quantitation of cytomegalovirus (CMV) DNA load in specimens other than blood such as bronchoalveolar lavages, intestinal biopsies, or urine has become a common practice as an ancillary tool for the diagnosis of CMV pneumonitis, intestinal disease, or ...
Eliseo Albert, Estela Giménez, Juan Alberola, Ignacio Torres, Yolanda López, Ana Marcos, Birgit Reinhardt, David Navarro   +7 more
doaj   +2 more sources

Application of realtime pcr in genotyping of hepatitis b virus at dak lak

Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2011
Hepatitis B is a disease caused by the hepatitis B virus (HBV), and can be acute or chronic. Infection with hepatitis B virus is the leading cause of chronic hepatitis worldwide and people with chronic hepatitis B infection are at increased risk for ...
Đỗ Thị Thùy Dương, Lê Huyền Ái Thúy   +1 more
doaj   +1 more source

Realtime-PCR [PDF]

Springer Reference Medizin, 2019
exaly   +2 more sources

Comparison of Zaire ebolavirus realtime RT-PCRs targeting the nucleoprotein gene [PDF]

Journal of Virological Methods, 2020
In last five years, the Africa has faced two outbreaks of Zaire ebolavirus. These outbreaks have been the largest so far, and latest outbreak is still ongoing and affecting the Democratic Republic of the Congo. We tested in parallel three different Zaire ebolavirus (EBOV) realtime RT-PCRs targeting the nucleoprotein gene (EBOV NP-RT-qPCRs) described by
Jääskeläinen, Anne J, Sironen, Tarja, Kaloinen, Minttu, Kakkola, Laura, Julkunen, Ilkka, Hewson, Roger, Weidmann, Manfred, Mirazimi, Ali, Watson, Robert, Vapalahti, Olli   +9 more
openaire   +6 more sources