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Realtime PCR Is More Sensitive than Multiplex PCR for Diagnosis and Serotyping in Children with Culture Negative Pneumococcal Invasive Disease [PDF]
BACKGROUND: Pneumococcal serotyping is usually performed by Quellung reaction, considered the gold standard test. However the method cannot be used on culture-negative samples. Molecular methods can be a useful alternative.
Massimo Resti +2 more
exaly +9 more sources
Establisment of realtime pcr protocol based on 16s rrna gene of mycobacterium tuberculosis
Currently, tuberculosis is still one of the most common infectious diseases, mainly caused by Mycobacterium tuberculosis bacterium infection. PCR is a molecular biology technique used to detect infectious agents, especially viruses or bacteria with ...
Lê Huyền Ái Thúy +2 more
doaj +2 more sources
Optimize realtime PCR reaction to detect Streptococcus agalactiae
Streptococcus agalactiae (GBS) is the major contagious cause of early-onset sepsis in newborns. Screening and intrapartum antibiotic prophylaxis for GBS in pregnant women could effectively prevent early-onset GBS infection in newborns.
Nguyễn Thị Thanh Thảo +3 more
doaj +2 more sources
Application of multiplex realtime PCR detection for hemorrhagic fever syndrome viruses
Background: Multiplex real-time PCR is a quick and cost effective method for detection of various gene simultaneously. HFSV (Hemorrhagic Fever Syndrome Virus) is a newly emerging infectious disease because of globalization and climate change. We tried to
Yoonhyuk Choi, Younghee Kim
doaj +3 more sources
Detection of bovine herpesvirus 4 DNA in cattle by realtime PCR
Introduction. BoHV-4 is poorly understood. Data on the circulation of the virus among animals and its role in infectious diseases insufficient. Aimes and goals.
A. V. Nefedchenko +5 more
doaj +4 more sources
Development of a realtime RT-PCR assay for the rapid detection of influenza A(H2) viruses
Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 1968, however, influenza A(H2) viruses
Mikhail Grudinin +2 more
exaly +3 more sources
Comparison of commercial realtime reverse transcription PCR assays for the detection of SARS-CoV-2
The emergence of a new coronavirus in Wuhan China has triggered a global need for accurate diagnostic assays. Initially, mostly laboratory developed molecular tests were available but shortly thereafter different commercial assays started to appear and are still increasing in number.
Zsófia Igloi +2 more
exaly +4 more sources
Application of realtime pcr in genotyping of hepatitis b virus at dak lak
Hepatitis B is a disease caused by the hepatitis B virus (HBV), and can be acute or chronic. Infection with hepatitis B virus is the leading cause of chronic hepatitis worldwide and people with chronic hepatitis B infection are at increased risk for ...
Đỗ Thị Thùy Dương +1 more
doaj +1 more source
Pilar Ruiz,1 Manuel Causse,2 Manuel Vaquero,1 Juan Bautista Gutierrez,1,2 Manuel Casal1,2 1Mycobacteria Reference Center, Department of Microbiology, Faculty of Medicine and Nursing, University of Córdoba, Córdoba, Spain; 2Microbiology ...
Ruiz P +4 more
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