Results 11 to 20 of about 802,113 (203)

Realtime PCR Is More Sensitive than Multiplex PCR for Diagnosis and Serotyping in Children with Culture Negative Pneumococcal Invasive Disease [PDF]

open access: yesPLoS ONE, 2010
BACKGROUND: Pneumococcal serotyping is usually performed by Quellung reaction, considered the gold standard test. However the method cannot be used on culture-negative samples. Molecular methods can be a useful alternative.
Massimo Resti   +2 more
exaly   +9 more sources

Establisment of realtime pcr protocol based on 16s rrna gene of mycobacterium tuberculosis

open access: yesTạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2011
Currently, tuberculosis is still one of the most common infectious diseases, mainly caused by Mycobacterium tuberculosis bacterium infection. PCR is a molecular biology technique used to detect infectious agents, especially viruses or bacteria with ...
Lê Huyền Ái Thúy   +2 more
doaj   +2 more sources

Optimize realtime PCR reaction to detect Streptococcus agalactiae

open access: yesTạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2021
Streptococcus agalactiae (GBS) is the major contagious cause of early-onset sepsis in newborns. Screening and intrapartum antibiotic prophylaxis for GBS in pregnant women could effectively prevent early-onset GBS infection in newborns.
Nguyễn Thị Thanh Thảo   +3 more
doaj   +2 more sources

Application of multiplex realtime PCR detection for hemorrhagic fever syndrome viruses

open access: yesJournal of Infection and Public Health, 2023
Background: Multiplex real-time PCR is a quick and cost effective method for detection of various gene simultaneously. HFSV (Hemorrhagic Fever Syndrome Virus) is a newly emerging infectious disease because of globalization and climate change. We tried to
Yoonhyuk Choi, Younghee Kim
doaj   +3 more sources

Detection of bovine herpesvirus 4 DNA in cattle by realtime PCR

open access: yesВопросы вирусологии, 2019
Introduction. BoHV-4 is poorly understood. Data on the circulation of the virus among animals and its role in infectious diseases insufficient. Aimes and goals.
A. V. Nefedchenko   +5 more
doaj   +4 more sources

Development of a realtime RT-PCR assay for the rapid detection of influenza A(H2) viruses

open access: yesMolecular and Cellular Probes, 2017
Influenza and other acute respiratory infections are of great concern for public health, causing excessive morbidity and mortality throughout the world. Influenza virus A(H2N2), which caused a pandemic of so called "Asian flu" in 1957 was expelled from the human population by the new pandemic virus subtype H3N2 in 1968, however, influenza A(H2) viruses
Mikhail Grudinin   +2 more
exaly   +3 more sources

Comparison of commercial realtime reverse transcription PCR assays for the detection of SARS-CoV-2

open access: yesJournal of Clinical Virology, 2020
The emergence of a new coronavirus in Wuhan China has triggered a global need for accurate diagnostic assays. Initially, mostly laboratory developed molecular tests were available but shortly thereafter different commercial assays started to appear and are still increasing in number.
Zsófia Igloi   +2 more
exaly   +4 more sources

Application of realtime pcr in genotyping of hepatitis b virus at dak lak

open access: yesTạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2011
Hepatitis B is a disease caused by the hepatitis B virus (HBV), and can be acute or chronic. Infection with hepatitis B virus is the leading cause of chronic hepatitis worldwide and people with chronic hepatitis B infection are at increased risk for ...
Đỗ Thị Thùy Dương   +1 more
doaj   +1 more source

Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis

open access: yesInfection and Drug Resistance, 2017
Pilar Ruiz,1 Manuel Causse,2 Manuel Vaquero,1 Juan Bautista Gutierrez,1,2 Manuel Casal1,2 1Mycobacteria Reference Center, Department of Microbiology, Faculty of Medicine and Nursing, University of Córdoba, Córdoba, Spain; 2Microbiology ...
Ruiz P   +4 more
doaj   +1 more source

Realtime-PCR [PDF]

open access: yesSpringer Reference Medizin, 2019
exaly   +2 more sources

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