Results 1 to 10 of about 2,483 (129)

High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

Nature Communications, 2019
The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates ...
Jeffrey L Bessen, Vlado Dancik, Luke W Koblan   +2 more
exaly   +2 more sources

Zinc Finger Recombinases with Adaptable DNA Sequence Specificity [PDF]

PLoS ONE, 2011
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase ...
Chris Proudfoot, Andreas F Kolb, W Marshall Stark   +2 more
exaly   +7 more sources

Founder transformants of cotton (Gossypium hirsutum L.) obtained through the introduction of DS-Red, Rec, Rep and CRISPR/Cas9 expressing constructs for developing base lines of recombinase mediated gene stacking [PDF]

PLOS ONE, 2022
Cotton being the major fiber crop across the world is exposed to numerous biotic and abiotic stresses. Genetic transformation of cotton is vital to meet the world’s food, feed and fiber demands. Genetic manipulation by randomly transferring the genes emanate variable gene expression.
Sabin Aslam, Sultan Habibullah Khan, Aftab Ahmad, Sriema Lalani Walawage, Abhaya M. Dandekar   +4 more
openaire   +5 more sources

Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors. [PDF]

, 2020
The Cre-loxP system is invaluable for spatial and temporal control of gene knockout, knockin, and reporter expression in the mouse nervous system. However, we report varying probabilities of unexpected germline recombination in distinct Cre driver lines ...
Abumaria, Nashat, Ambrozkiewicz, Mateusz C, Beier, Kevin T, Benseler, Fritz, Brose, Nils, Burgess, Harold A, Cepko, Constance L, Chen, Cui, Cloutier, Jean-François, Craig, Ann Marie, Dumontier, Emilie, Eroglu, Cagla, Falkner, Susanne, Furlanis, Elisabetta, Goebbels, Sandra, Gomez, Andrea M, Hoshina, Naosuke, Huang, Wei-Hsiang, Hutchison, Mary Anne, Itoh-Maruoka, Yu, Kaeser, Pascal S, Kawabe, Hiroshi, Kay, Jeremy N, Lavery, Laura A, Li, Wei, Lu, Wei, Luo, Lin, Luo, Liqun, Mandai, Kenji, Maruo, Tomohiko, McBain, Chris J, Motohashi, Junko, Nave, Klaus-Armin, Pai, Emily Ling-Lin, Pelkey, Kenneth A, Pereira, Ariane, Philips, Thomas, Prado, Marco AM, Prado, Vania F, Rothstein, Jeffrey, Rubenstein, John LR, Saher, Gesine, Sakimura, Kenji, Sanes, Joshua R, Scheiffele, Peter, Sinclair, Jennifer L, Stogsdill, Jeff A, Takai, Yoshimi, Traunmüller, Lisa, Umemori, Hisashi, Verhage, Matthijs, Wang, Jiexin, Wortel, Joke, You, Wenjia, Yuzaki, Michisuke, Zoghbi, Huda Yahya   +55 more
core   +2 more sources

Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]

, 2012
Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi, Coates, Craig J, Dang, Nong C, Maragathavally, Kommineni J, Moisyadi, Stefan, Owens, Jesse B, Segal, David J, Stoytchev, Ilko, Stoytcheva, Zoia, Urschitz, Johann   +9 more
core   +5 more sources

Detecting variable (V), diversity (D) and joining (J) gene segment recombination using a two-colour fluorescence system [PDF]

, 2010
BACKGROUND: Diversity of immunoglobulins and the T cell antigen receptors is achieved via the recombination activating gene (RAG)-mediated rearrangement of variable (V), diversity (D) and joining (J) gene segments, and this underpins the efficient ...
Erika A de Wynter, Gina B Scott, Graham P Cook   +2 more
core   +4 more sources

The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation [PDF]

, 2014
We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or
A Maas, A Maas, Alexander W. Tucker, Andrew N. Rycroft, B Zhan, Brendan W. Wren, C O'Neill, Denise M. Soares-Bazzolli, Duncan J. Maskell, EA Izano, FF Buettner, G Li, Glenn Francis Browning, H Maughan, I Jacobsen, I Jacobsen, Janine T. Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, L Lin, LM Mullen, M Copass, M Ramjeet, N Baltes, N Baltes, N Baltes, N Baltes, N Baltes, Paul R. Langford, PJ Blackall, R Jansen, RJ Redfield, RM Jones, RV Joshi, SJ Foote, T Ali, TJ Inzana, W Oswald, W Tonpitak, W Tonpitak, WW Fung, Yanwen Li, Z Xu   +46 more
core   +5 more sources

The chondro-osseous continuum: is it possible to unlock the potential assigned within? [PDF]

, 2018
Endochondral ossification (EO), by which long bones of the axial skeleton form, is a tightly regulated process involving chondrocyte maturation with successive stages of proliferation, maturation, and hypertrophy, accompanied by cartilage matrix ...
Adams, Aizawa, Akintoye, Amling, Bahney, Ballock, Behonick, Boegård, Brachet, Cancedda, Candeliere, Carames, Clarkin, Colnot, Colnot, Day, Dodds, Emons, Enishi, Erenpreisa, Farnum, Farnum, Felson, Galotto, Gebhard, Gerstenfeld, Gibson, Gibson, Hall, Holmbeck, Hu, Hughes, Hunter, Javaheri, Jing, Johnell, Karaplis, Kawaguchi, Kuttapitiya, Lavigne, Lian, Lieu, Mackie, Maeda, Maes, Maes, Marigo, Moskalewski, Murphy, Ono, Ortega, Park, Pottenger, Poulet, Provot, Rawlinson, Riminucci, Roach, Roach, Roach, Shah, Shapiro, Silbermann, Song, Stafford, Stickens, Thesingh, Tsang, Van der Stricht, Vortkamp, Vu, Wade-Gueye, Wang, Woolf, Yang, Yoshioka, Zelzer, Zenmyo, Zhou, Zhou   +79 more
core   +3 more sources

The mechanism of ϕC31 integrase directionality : experimental analysis and computational modelling [PDF]

, 2016
Serine integrases, DNA site-specific recombinases used by bacteriophages for integration and excision of their DNA to and from their host genomes, are increasingly being used as tools for programmed rearrangements of DNA molecules for biotechnology and ...
Colloms, Sean D, Ebenhöh, Oliver, Pokhilko, Alexandra, Smith, Margaret C M, Stark, W Marshall, Zhao, Jia   +5 more
core   +3 more sources

RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator [PDF]

, 2017
Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity.
Allers, Allers, Bernstein, Bitan-Banin, Cardenas, Clark, Cooper, Delmas, Esashi, Fortin, Galkin, Graham, Greg H.P. Ngo, Guy, Haldenby, Hayashi, Kayleigh Wardell, Kim, Komori, Kyte, Lavery, Lestini, Liu, Liu, Marmorstein, McEntee, Mevarech, Monnat, Mortier-Barriere, Nathan Jones, Renzette, Sakai, Sam Haldenby, Shin, Stroud, Sugiyama, Susan Liddell, Taylor, Thompson, Thorsten Allers, Wang, Woods   +41 more
core   +2 more sources