Results 1 to 10 of about 1,681 (87)

High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

Nature Communications, 2019
The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates ...
Jeffrey L Bessen, Vlado Dancik, Luke W Koblan   +2 more
exaly   +2 more sources

Founder transformants of cotton (Gossypium hirsutum L.) obtained through the introduction of DS-Red, Rec, Rep and CRISPR/Cas9 expressing constructs for developing base lines of recombinase mediated gene stacking [PDF]

PLOS ONE, 2022
Cotton being the major fiber crop across the world is exposed to numerous biotic and abiotic stresses. Genetic transformation of cotton is vital to meet the world’s food, feed and fiber demands. Genetic manipulation by randomly transferring the genes emanate variable gene expression.
Sabin Aslam, Sultan Habibullah Khan, Aftab Ahmad, Sriema Lalani Walawage, Abhaya M. Dandekar   +4 more
openaire   +5 more sources

Precise excision of HTLV-1 provirus with a designer-recombinase. [PDF]

Mol Ther, 2023
Rojo-Romanos T, Karpinski J, Millen S, Beschorner N, Simon F, Paszkowski-Rogacz M, Lansing F, Schneider PM, Sonntag J, Hauber J, Thoma-Kress AK, Buchholz F.   +11 more
europepmc   +1 more source

Discovery and characterization of novel Cre-type tyrosine site-specific recombinases for advanced genome engineering. [PDF]

Nucleic Acids Res, 2023
Jelicic M, Schmitt LT, Paszkowski-Rogacz M, Walder A, Schubert N, Hoersten J, Sürün D, Buchholz F.   +7 more
europepmc   +1 more source

Construction of a bacteriophage-derived recombinase system in Bacillus licheniformis for gene deletion. [PDF]

AMB Express, 2023
Xue F, Ma X, Luo C, Li D, Shi G, Li Y.
europepmc   +1 more source

Linear Dichroism Measurements for the Study of Protein-DNA Interactions. [PDF]

Int J Mol Sci, 2023
Takahashi M, Norden B.
europepmc   +1 more source

EMMA: a new method for computing multiple sequence alignments given a constraint subset alignment. [PDF]

Algorithms Mol Biol, 2023
Shen C, Liu B, Williams KP, Warnow T.
europepmc   +1 more source

Computational Insights into the Dynamic Structural Features and Binding Characteristics of Recombinase UvsX Compared with RecA. [PDF]

Molecules, 2023
Pan Y, Xie N, Zhang X, Yang S, Lv S.
europepmc   +1 more source

Identification of cognate recombination directionality factors for large serine recombinases by virtual pulldown. [PDF]

Nucleic Acids Res
Shin H, Holland A, Alsaleh A, Retiz AD, Pigli YZ, Taiwo-Aiyerin OT, Peña Reyes T, Bello AJ, Quan J, Tang W, Olorunniji FJ, Rice PA.   +11 more
europepmc   +2 more sources

Large serine integrases utilise scavenged phage proteins as directionality cofactors. [PDF]

Nucleic Acids Res
Alsaleh A, Holland A, Shin H, Reyes TP, Baksh A, Taiwo-Aiyerin OT, Pigli Y, Rice PA, Olorunniji FJ.   +8 more
europepmc   +2 more sources