Results 11 to 20 of about 2,483 (129)

Validation of Hexaplex Recombinase Polymerase Amplification Assay for the Detection of Carbapenemase Genes in Klebsiella pneumoniae Isolates From Pediatric Inpatients. [PDF]

J Clin Lab Anal
A novel hexaplex recombinase polymerase amplification (RPA) method was developed to rapidly and accurately detect five key carbapenemase genes and a Klebsiella pneumoniae‐specific target in a single reaction. This makes it an effective tool for CRKP detection in resource‐constrained environments.
Mutitanon S, Chaichanawongsaroj N.
europepmc   +2 more sources

c‐Rel–dependent Chk2 signaling regulates the DNA damage response limiting hepatocarcinogenesis

Hepatology, EarlyView., 2022
In response to genotoxic injury, c‐Rel upregulates ATM‐Chk2‐p53 pathway DNA damage proteins to limiting hepatocarcinogenesis. Abstract Background and Aims Hepatocellular carcinoma (HCC) is a leading cause of cancer‐related death. The NF‐κB transcription factor family subunit c‐Rel is typically protumorigenic; however, it has recently been reported as a
Jack Leslie, Jill E. Hunter, Amy Collins, Amelia Rushton, Lauren G. Russell, Erik Ramon‐Gil, Maja Laszczewska, Misti McCain, Marco Y. W. Zaki, Amber Knox, Yixin Seow, Laura Sabater, Daniel Geh, Neil D. Perkins, Helen L. Reeves, Dina Tiniakos, Derek A. Mann, Fiona Oakley   +17 more
wiley   +1 more source

Advances in Double-Stranded DNA Targeting Technologies. [PDF]

Exploration (Beijing)
Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Shen Z, Liu Y, Hao Y, Bo Y, Dai X, Wang S, Xia T, Su X, Liu H.   +8 more
europepmc   +2 more sources

Phase and antigenic variation in mycoplasmas [PDF]

, 2010
With their reduced genome bound by a single membrane, bacteria of the Mycoplasma species represent some of the simplest autonomous life forms. Yet, these minute prokaryotes are able to establish persistent infection in a wide range of hosts, even in the ...
Baranowski, Eric, Citti, Christine, Nouvel, Laurent-Xavier   +2 more
core   +2 more sources

RAG Recombinase as a Selective Pressure for Genome Evolution [PDF]

, 2016
The RAG recombinase is a domesticated transposable element co-opted in jawed vertebrates to drive the process of the so-called V(D)J recombination, which is the hallmark of the adaptive immune system to produce antigen receptors. RAG targets, namely, the
Athanasiadis, A., Barreto, V.M., Bonnet, M., Demengeot, J., Passagem-Santos, D., Pereira-Leal, J.B., Silva, J.G., Sobral, D., Trancoso, I.   +8 more
core   +1 more source

Rapid, modular and reliable construction of complex mammalian gene circuits [PDF]

, 2013
We developed a framework for quick and reliable construction of complex gene circuits for genetically engineering mammalian cells. Our hierarchical framework is based on a novel nucleotide addressing system for defining the position of each part in an ...
Andronescu, Andronescu, Ausländer, Botev, Brown, Bushey, Drummond, Gibson, Gibson, Godiska, Hockemeyer, Ishihara, Kriz, Li, Liliana Wroblewska, Litcofsky, Livet, Patrick Guye, Ron Weiss, Ruder, Sarrion-Perdigones, Sasaki, Schmid-Burgk, Shortreed, Slusarczyk, Sprinzak, Vierbuchen, Weber, Xavier Duportet, Xie, Yahata, Yinqing Li   +31 more
core   +4 more sources

Recombination directionality factor gp3 binds ϕC31 integrase via the zinc domain, potentially affecting the trajectory of the coiled-coil motif [PDF]

, 2017
To establish a prophage state, the genomic DNA of temperate bacteriophages normally becomes integrated into the genome of their host bacterium by integrase-mediated, site-specific DNA recombination.
Fernando, Booshini D, Fogg, Paul C M, Khaleel, Thanafez, Smith, Margaret C M, Stark, W Marshall, Younger, Ellen   +5 more
core   +2 more sources

ICEApl1, an integrative conjugative element related to ICEHin1056, identified in the pig pathogen Actinobacillus pleuropneumoniae [PDF]

, 2016
ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline-resistant (MIC = 8 mg/L) serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the
Blanco, Blanco, Bossé, Bossé, Bossé, Boyd, Burrus, Clinical and Laboratory Standards Institute [CLSI], Dimopoulou, Dimopoulou, Eidam, Elhai, European Medicines Agency, Garneau, Guglielmini, Howell, Juhas, Juhas, Juhas, Lin, Michael, Michael, Mohd-Zain, O’Neill, Sentchilo, Sárközi, Wozniak   +26 more
core   +5 more sources

Classification and evolutionary history of the single-strand annealing proteins, RecT, Redβ, ERF and RAD52 [PDF]

, 2002
Background The DNA single-strand annealing proteins (SSAPs), such as RecT, Redβ, ERF and Rad52, function in RecA-dependent and RecA-independent DNA recombination pathways. Recently, they have been shown to form similar helical quaternary superstructures.
AI Roca, PR Bianco, A Kuzminov, P Noirot, SC Kowalczykowski, F Paques, HW Chang, SD Barbour, SR Kushner, R Kolodner, SD Hall, SD Hall, UH Mortensen, SI Passy, RJ Thresher, A Shinohara, AR Poteete, B Stevenson, UH Stroeher, E Kmiec, D Botstein, S Weaver, AR Poteete, AR Poteete, AR Poteete, AZ Stasiak, X Shao, AJ Doherty, L Aravind, YI Wolf, M Huynen, T Dandekar, L Aravind, L Aravind, JC Connelly, JC Connelly, C Bruand, SF Altschul, C Notredame, B Rost, B Rost, JA Cuff, LA Kelley, D Fischer, YI Wolf, J Adachi, J Felsenstein   +46 more
core   +1 more source

Polyploid superficial cells that maintain the urothelial barrier are produced via incomplete cytokinesis and endoreplication [PDF]

, 2018
Summary: The urothelium is an epithelia barrier lined by a luminal layer of binucleated, octoploid, superficial cells. Superficial cells are critical for production and transport of uroplakins, a family of proteins that assemble into a waterproof ...
Batourina, Ekatherina, Canman, Julie C, Dan, Hanbin, George, Christopher D, Liu, Chang, Mendelsohn, Cathy Lee, Mysorekar, Indira U, Schneider, Kerry, Souza, Spenser, Swayne, Theresa, Tate, Tiffany, Wang, Jia, Wiessner, Gregory, Zhuravlev, Yelena   +13 more
core   +3 more sources