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Characteristics of the cytoplasmic and nuclear dihydrotestosterone receptors of human thymic tissue
Steroids, 1983Dihydrotestosterone receptor was prepared from both the cytoplasm and nucleus of human thymic tissue. Human cytoplasmic and nuclear dihydrotestosterone receptor possessed a KA greater than 1.0 and by competition assay was highly specific for dihydrotestosterone.
C J, Grossman +2 more
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Nuclear and cytoplasmic lectin receptor sites in rat Py1a osteoblasts.
Histology and histopathology, 2000The intracellular distribution of lectin receptor sites was studied in the rat Pyla osteoblasts using immunofluorescence at the confocal microscopy level. This immortalized cell line was found to represent a satisfactory model to study the occurrence and distribution of sugar moieties.
SABBIETI, Maria Giovanna +3 more
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Characteristics of Cytoplasmic and Nuclear Receptor Forms
1979Although this monograph is primarily concerned with the relationship between hormone-induced responses and nuclear binding, it is necessary to relate nuclear receptors to their cytoplasmic counterparts.
James H. Clark, Ernest J. Peck
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Cytoplasmic and nuclear signaling pathways of V1-vascular vasopressin receptors
Regulatory Peptides, 1993We studied the cytoplasmic and nuclear signaling pathways of V1-vascular AVP receptors of human platelets, primary cultures of renal glomerular mesangial cells, and established cultures of the A7r5 aortic smooth muscle cell line. The immediate transmembrane signals are triggered by the formation of ligand-receptor complexes as illustrated by binding ...
M, Thibonnier, A L, Bayer, Z, Leng
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Endocrinology, 1987
Using a monospecific, monoclonal antibody against the glucocorticoid receptor (GR), an immunocytochemical study was performed to investigate the intracellular localization of GR both in the presence or absence of ligand. With all fixation methods tested (paraformaldehyde, acetic acid in ethanol, Bouin's fixative, and bensochinone in PBS), it was ...
A C, Wikström +4 more
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Using a monospecific, monoclonal antibody against the glucocorticoid receptor (GR), an immunocytochemical study was performed to investigate the intracellular localization of GR both in the presence or absence of ligand. With all fixation methods tested (paraformaldehyde, acetic acid in ethanol, Bouin's fixative, and bensochinone in PBS), it was ...
A C, Wikström +4 more
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Gynecologic Oncology, 1990
Biopsies from 131 women with squamous cell carcinoma of the cervix diagnosed between May 1983 and July 1986 were assayed for nuclear and "cytoplasmic" estrogen receptors (NER and CER). Progesterone receptors (PR) were also assayed in 45 of these tumors. About a third of the tumors contained both CER and NER. One or the other fraction contained ER in 76.
J, Darne +3 more
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Biopsies from 131 women with squamous cell carcinoma of the cervix diagnosed between May 1983 and July 1986 were assayed for nuclear and "cytoplasmic" estrogen receptors (NER and CER). Progesterone receptors (PR) were also assayed in 45 of these tumors. About a third of the tumors contained both CER and NER. One or the other fraction contained ER in 76.
J, Darne +3 more
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Biochemistry, 1981
Highly purified activated cytoplasmic glucocorticoid hormone receptor binds with high affinity to sites in the nuclear envelope. Nuclear envelope fragments can be isolated from purified chromatin. They bind activated cytoplasmic glucocorticoid receptor with the same equilibrium constant as nuclear envelopes.
P, Smith, C, von Holt
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Highly purified activated cytoplasmic glucocorticoid hormone receptor binds with high affinity to sites in the nuclear envelope. Nuclear envelope fragments can be isolated from purified chromatin. They bind activated cytoplasmic glucocorticoid receptor with the same equilibrium constant as nuclear envelopes.
P, Smith, C, von Holt
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Journal of Steroid Biochemistry, 1980
Abstract A controlled trypsinization procedure is reported which results in more efficient solubilization of the nuclear estrogen receptor from human endometrium and rat uterine tissue than does extraction with high salt (0.4 M KCl) alone. The trypsin-liberated nuclear receptors in both species sediment at 3–4S on both high and low salt gradients ...
B S, Katzenellenbogen +2 more
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Abstract A controlled trypsinization procedure is reported which results in more efficient solubilization of the nuclear estrogen receptor from human endometrium and rat uterine tissue than does extraction with high salt (0.4 M KCl) alone. The trypsin-liberated nuclear receptors in both species sediment at 3–4S on both high and low salt gradients ...
B S, Katzenellenbogen +2 more
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Molecular pathways involved in the transport of nuclear receptors from the nucleus to cytoplasm
The Journal of Steroid Biochemistry and Molecular Biology, 2018Nuclear receptors (NRs) are transcription regulators that direct the expression of many genes linked to cellular processes, such as proliferation, differentiation, and apoptosis. Additionally, some cellular events are also modulated by signaling pathways induced by NRs outside of the nucleus.
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Journal of Steroid Biochemistry, 1985
An assay for the quantitation of cytoplasmic and nuclear glucocorticoid receptors in lymphoid tissue has been developed using controlled pore glass (CPG) beads. Soluble receptor--3H-steroid complex (cytosol or nuclear extract) is adsorbed quantitatively within the crevasses of porous glass beads.
G J, Gormley +3 more
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An assay for the quantitation of cytoplasmic and nuclear glucocorticoid receptors in lymphoid tissue has been developed using controlled pore glass (CPG) beads. Soluble receptor--3H-steroid complex (cytosol or nuclear extract) is adsorbed quantitatively within the crevasses of porous glass beads.
G J, Gormley +3 more
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