Results 61 to 70 of about 3,076,380 (339)

Photocontrol of Endogenous Glycine Receptors In Vivo [PDF]

Cell Chemical Biology, 2020
Glycine receptors (GlyRs) are indispensable for maintaining excitatory/inhibitory balance in neuronal circuits that control reflexes and rhythmic motor behaviors. Here we have developed Glyght, a GlyR ligand controlled with light. It is selective over other Cys-loop receptors, is active in vivo, and displays an allosteric mechanism of action.
Alexandre M.J. Gomila, Karin Rustler, Galyna Maleeva, Alba Nin-Hill, Daniel Wutz, Antoni Bautista-Barrufet, Xavier Rovira, Miquel Bosch, Elvira Mukhametova, Elena Petukhova, Daria Ponomareva, Marat Mukhamedyarov, Franck Peiretti, Mercedes Alfonso-Prieto, Carme Rovira, Burkhard König, Piotr Bregestovski, Pau Gorostiza   +17 more
openaire   +6 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single-channel behavior of heteromeric α1β glycine receptors: an attempt to detect a conformational change before the channel opens [PDF]

, 2004
The α1β heteromeric receptors are likely to be the predominant synaptic form of glycine receptors in the adult. Their activation mechanism was investigated by fitting putative mechanisms to single-channel recordings obtained at four glycine ...
Beato, M., Burzomato, V., Colquhoun, D., Groot-Kormelink, P.J., Sivilotti, L.G.   +4 more
core  

Conformational variability of the glycine receptor M2 domain in response to activation by different agonists [PDF]

, 2007
Models describing the structural changes mediating cys-loop receptor activation generally give little attention to the possibility that different agonists may promote activation via distinct M2 pore-lining domain structural rearrangements.
Akabas, Chang, Cymes, Cymes, Dahan, England, Gandhi, Grosman, Han, Hawthorne, Hawthorne, Hemmings, Kondratskaya, Kondratskaya, Law, Lee, Lewis, Lobo, Lynch, Lynch, Lynch, Miyazawa, Muroi, O'Shea, Paas, Rajendra, Shan, Unwin, Unwin, Webb, Wilson   +30 more
core   +3 more sources

Cannabinoids suppress inflammatory and neuropathic pain by targeting α3 glycine receptors

Journal of Experimental Medicine, 2012
Systemic and intrathecal administration of derivatives of a nonpsychoactive component of marijuana significantly suppresses chronic inflammatory and neuropathic pain, without causing analgesic tolerance, in several rodent models.
W. Xiong, Tanxing Cui, Kejun Cheng, Fei Yang, Shao-Rui Chen, D. Willenbring, Y. Guan, H. Pan, Ke Ren, Yan Xu, Li Zhang   +10 more
semanticscholar   +1 more source

BENZODIAZEPINES AND CENTRAL GLYCINE RECEPTORS [PDF]

British Journal of Pharmacology, 1976
In cats, anaesthetized with pentobarbitone, intravenous diazepam (minimum dose 3.0 mg/kg) enhanced dorsal root potentials but did not significantly diminish the reduction by electrophoretic strychnine of the inhibitory action of electrophoretic glycine on dorsal horn interneurones.
D R, Curtis, C J, Game, D, Lodge
openaire   +2 more sources

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Neurochemical characterisation of lamina II inhibitory interneurons that express GFP in the PrP-GFP mouse [PDF]

, 2013
Background Inhibitory interneurons in the superficial dorsal horn play important roles in modulating sensory transmission, and these roles are thought to be performed by distinct functional populations. We have identified 4 non-overlapping classes among
Garzillo, F., Iwagaki, N., Polgár, Erika, Riddell, J.S., Todd, A.J.   +4 more
core   +2 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Glycine-induced neurotoxicity in organotypic hippocampal slice cultures [PDF]

, 2018
The role of the neutral amino acid glycine in excitotoxic neuronal injury is unclear. Glycine coactivates glutamate N-methyl-D-aspartate (NMDA) receptors by binding to a distinct recognition site on the NR1 subunit.
Barth, A., Barth, L., Newell, D., Nguyen, L.   +3 more
core