Results 91 to 100 of about 532,969 (259)

De novo protein sequencing of antibodies for identification of neutralizing antibodies in human plasma post SARS-CoV-2 vaccination

Nature Communications
The antibody response to vaccination and infection is a key component of the immune response to pathogens. Sequencing of peripheral B cells may not represent the complete B cell receptor repertoire.
Thierry Le Bihan, Teresa Nunez de Villavicencio Diaz, Chelsea Reitzel, Victoria Lange, Minyoung Park, Emma Beadle, Lin Wu, Marko Jovic, Rosalin M. Dubois, Amber L. Couzens, Jin Duan, Xiaobing Han, Qixin Liu, Bin Ma   +13 more
doaj   +1 more source

Rapid generation of human recombinant monoclonal antibodies from antibody-secreting cells using ferrofluid-based technology

Frontiers in Immunology
Monoclonal antibodies (mAbs) are one of the most important classes of biologics with high therapeutic and diagnostic value, but traditional methods for mAbs generation, such as hybridoma screening and phage display, have limitations, including low ...
Veronica Strazza, Veronica Strazza, Marco Rossi, Andrea Avati, Giusy Tiseo, Marco Falcone, Maria Grazia Cusi, Francesco Menichetti, Paola Ricciardi-Castagnoli, Cristina Tinti, Piero Pileri   +10 more
doaj   +1 more source

Investigation of Conditions for Capture of Live Legionella pneumophila with Polyclonal and Recombinant Antibodies. [PDF]

Biosensors (Basel), 2022
Paladines L, Hassen WM, Chawich J, Dübel S, Lévesque S, Dubowski JJ, Frost EH.   +6 more
europepmc   +1 more source

A Chemical Framework for Engineering Extracellular Vesicles’ Biointerface to Advance Precision Therapeutics

Advanced Materials, EarlyView.
Extracellular vesicles (EVs) are cell‐derived nanoparticles that mediate intercellular communication through their dynamic biointerfaces. Owing to their intrinsic biological functions, EVs have emerged as promising platforms for biomedical applications.
Leila Pourtalebi Jahromi, Felix Geng, Jan‐Noël Frenzke, Gregor Fuhrmann   +3 more
wiley   +1 more source

De Novo Autogenic Engineered Living Functional Materials

Advanced Materials, EarlyView.
A versatile platform for de novo autogenic engineered living materials is presented, leveraging protein mining, computational modeling, and synthetic biology. By reprogramming the Escherichia coli curli machinery with β‐solenoid proteins from non‐model bacteria, macroscopic biomaterials with enhanced mechanical properties, UV‐C irradiation protection ...
Hoda M. Hammad, Seth Swarnadeep, Erin C. Jackson, Nicolas Burns, Hongyu Wang, Harrison Priode, Robert B. Moore, Sanket Deshmukh, Avinash Manjula‐Basavanna, Anna M. Duraj‐Thatte   +9 more
wiley   +1 more source

Application of recombinant antibodies for treatment of Clostridioides difficile infection: Current status and future perspective. [PDF]

Front Immunol, 2022
Raeisi H, Azimirad M, Nabavi-Rad A, Asadzadeh Aghdaei H, Yadegar A, Zali MR.   +5 more
europepmc   +1 more source

Ultrathin Hydrogel Membranes Inspired by Soap Films Enable Physiologically Relevant Breathing Lung Models

Advanced Materials, EarlyView.
A soap‐film inspired strategy enables the fabrication of ultrathin yet robust GelMA‐PAAm membranes for breathing lung culture. The hydrogel membrane supports long‐term cyclic deformation under air‐liquid interface conditions, enabling physiologically relevant breathing motions of engineered lung tissue culture. ABSTRACT Breathing continuously stretches
Yunji Lee, Gwang Myeong Kim, Wookyeom Kim, Fenny Soetanto, Gyungin Ryu, Taehun Chung, Youn Soo Kim, Junmin Lee, Hwa‐Rim Lee, Sungjune Jung   +9 more
wiley   +1 more source

Construction of gene recombinant IgG1 κ anti-P plasmids and their expression in HEK293T Cells

Zhongguo shuxue zazhi
[Objective] To construct gene recombinant expression plasmids of human anti-P antibody with IgG1 and kappa chain based on the human hybridoma cell line.
GUO Zhonghui, ZHANG Jiamin, ZHU Xinyi, YANG Ying, ZHU Ziyan   +4 more
doaj   +1 more source

Development of a CHO cell line for stable production of recombinant antibodies against human MMP9. [PDF]

BMC Biotechnol, 2022
Ryu J, Kim EJ, Kim JK, Park TH, Kim BG, Jeong HJ.   +5 more
europepmc   +1 more source

Metal–Organic Framework‐Preserved Thermostable Microneedle Patch for Minimally Invasive Detection and Monitoring of Kidney Dysfunction

Advanced Materials, EarlyView.
By growing a conformal ZIF‐8 protective layer directly on antibody‐functionalized microneedles, this work converts a fragile biosensing interface into a thermostable diagnostic platform. The MOF@MN patch enables minimally invasive NGAL detection from dermal ISF while supporting cold‐chain‐independent storage and transport for decentralized kidney ...
Yixuan Wang, Ying Liu, Avishek Debnath, Chuang Li, Zheyu Wang, Gayathri Kondepati, Saketh Balmoori, Jeremiah J. Morrissey, Ying Maggie Chen, Limei Tian, Srikanth Singamaneni   +10 more
wiley   +1 more source