Results 71 to 80 of about 532,969 (259)

Encoded Cell‐Material Interactions to Reroute Cytokine Signaling for Regenerative Medicine

Advanced Healthcare Materials, EarlyView.
We present native MATRIX (Material Activated To Regulate Inducible gene eXpression), a co‐engineered material‐cell platform. Surfaces functionalized with antibodies for endogenous soluble ligands (e.g., IL‐1β or IL‐6) capture and immobilize ligands for presentation to a cognate, engineered synthetic Notch receptor, releasing a transcription factor (TF)
Zachary M. Eidman, Jhanvi Sharma, Joanne C. Lee, Nicholas F. Schulze, Hannah J. Brien, Kevin C. Corn, Marjan Rafat, Jonathan M. Brunger   +7 more
wiley   +1 more source

Construction of an Integration Vector with a Chimeric Signal Peptide for the Expression of Monoclonal Antibodies in Mammalian Cells

Current Issues in Molecular Biology
Antibodies are complex protein structures, and producing them using eukaryotic expression systems presents significant challenges. One frequently overlooked aspect of expression vectors is the nucleotide sequence encoding the signal peptide, which plays ...
Valentina S. Nesmeyanova, Daniil V. Shanshin, Denis E. Murashkin, Dmitriy N. Shcherbakov   +3 more
doaj   +1 more source

A Fully Defined GelMA‐Based Matrix Allows Fine Tuning of Tissue‐Relevant Biomechanical and Biochemical Cues for Organoid Culture

Advanced Healthcare Materials, EarlyView.
A fully defined GelMA‐based ECM platform integrates tunable tissue‐relevant stiffness with laminin–entactin biochemical supplementation to support mouse small intestinal organoid growth. Coordinated control of mechanical and biochemical cues promotes robust proliferation, budding morphogenesis, differentiation, and passaging, providing a reproducible ...
Junyi Liu, Su Su Htwe, Lay Poh Tan, Yen Choo   +3 more
wiley   +1 more source

The Use of Heterologous Antigens for Biopanning Enables the Selection of Broadly Neutralizing Nanobodies Against SARS-CoV-2

Antibodies
Background: Since the emergence of SARS-CoV-2 in the human population, the virus genome has undergone numerous mutations, enabling it to enhance transmissibility and evade acquired immunity.
Vazirbek S. Aripov, Anna V. Zaykovskaya, Ludmila V. Mechetina, Alexander M. Najakshin, Alexander A. Bondar, Sergey G. Arkhipov, Egor A. Mustaev, Margarita G. Ilyina, Sophia S. Borisevich, Alexander A. Ilyichev, Valentina S. Nesmeyanova, Anastasia A. Isaeva, Ekaterina A. Volosnikova, Dmitry N. Shcherbakov, Natalia V. Volkova   +14 more
doaj   +1 more source

Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetic acid hapten using naďve phage library

Veterinární Medicína, 2003
Three single chain variable fragment (scFv) antibodies against 2,4-dichlophenoxyacetic acid (2,4-D) herbicide were produced by the Griffin1.library.
J. Brichta, H. Vesela, M. Franek
doaj   +1 more source

Vascularization of Human iPSC‐Derived Kidney Organoids Using Perfusion Culture, Pre‐Vascularized Collagen Scaffolds, and Decellularized Extracellular Matrix

Advanced Healthcare Materials, EarlyView.
An integrated perfusion‐based culture platform combining pre‐vascularized collagen scaffolds, stromal vascular fraction cells, and kidney decellularized extracellular matrix promotes vascularization of human iPSC‐derived kidney organoids. The platform provides evidence of interactions between vascular networks and glomerular‐like structures and enables
Helen Kearney, Andrea Mazzoleni, Ivan Martin, Lorenzo Moroni, Manuele Giuseppe Muraro, Carlos Mota   +5 more
wiley   +1 more source

Nanomaterial Strategies for Pulmonary Delivery of Immunotherapeutics in Lung Cancer Treatment

Advanced Healthcare Materials, EarlyView.
Inhalable immunotherapeutic nanomedicines enable organ‐selective immune modulation by overcoming pulmonary delivery barriers and concentrating therapy within lung tumors. This Review defines how nanomaterial properties govern airway deposition, retention, cellular partitioning, and immune activation across vaccines, checkpoint blockade, STING agonists,
Han Zhang, Wei Tang
wiley   +1 more source

Inhibition of VEGF‐Induced Angiogenesis and Vascular Leakage by Bicistronic Co‐Expression of Aflibercept and COMP‐Ang1

Advanced Healthcare Materials, EarlyView.
A bicistronic rAAV8 vector encoding Aflibercept (Afb) and COMP‐cAng1 simultaneously coordinates VEGF clearance and Tie2‐mediated vessel maturation. In a 3D angiogenesis‐on‐a‐chip model, rAAV8‐Afb/cAng1 effectively reverses pathological barrier breakdown under clinical disease‐mimicking challenges.
Bong‐Kyu Kim, Jaehong Min, Sang‐Hee Park, Subeen Shin, Sangbin Yu, Jeong‐Eun Park, Sujin Kim, Wooju Park, Jihoon Ko, Sunjong Kwon   +9 more
wiley   +1 more source

Monoclonal antibody techniques. 50 years of development

Журнал микробиологии, эпидемиологии и иммунобиологии
Monoclonal antibodies are widely used in all fields of biology and medicine. The emergence and development of the technology for their production revolutionized immunology and allowed the creation of not only new diagnostic methods, but also many ...
Yulia S. Massino, Yulia N. Tarakanova, Olga L. Segal, Anastasia A. Pechelyulko, Dinora A. Yakovleva, Maria V. Lichutina, Dmitriy A. Dmitriev, Alexander D. Dmitriev   +7 more
doaj   +1 more source

Controlling ion channel function with renewable recombinant antibodies. [PDF]

J Physiol, 2022
Colecraft HM, Trimmer JS.
europepmc   +1 more source