Results 101 to 110 of about 1,379,490 (255)
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Nano Biomedicine and Engineering, 2009 Human Neurobeachin-like 1(NBEAL1) gene was an important member of BEACH-WD40 domain family, which was confirmed to be overexpressed in Ⅰ stage glioma. In this study, we extracted total RNAs from U251 cell line, acquired its cDNA sequence by RT-PCR, and ...
Chenchen Bao +9 more
doaj +1 more source
FEBS Open Bio, EarlyView.The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez +4 more
wiley +1 more source
Improved methods for production and characterisation of Jembrana disease virus proteins [PDF]
, 2010 Jembrana disease is an acute disease of Bali cattle (Bos javanicus) in Indonesia caused by Jembrana disease virus (JDV), a lentivirus most closely related to Bovine immunodeficiency virus. Control of the disease in Bali cattle, which are important to the
Rachmat, Judhi
core
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
FEBS Open Bio, EarlyView.Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
The RB611 recombinant antibody recognizes human and murine pancreatic polypeptide by immunofluorescence [PDF]
, 2021 A new recombinant antibody, RB611, detects human and murine pancreatic polypeptide (PPY) by immunofluorescence on histological ...
Thorel, Fabrizio +2 more
core
FEBS Open Bio, EarlyView.TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao +10 more
wiley +1 more source
, 2014 Vigilin, a highly conserved protein from yeast to mammals, is a multifunctional protein in eukaryotic organisms. One biological function of vigilin is to stabilize the expression level of vitellogenin (VTG).
Yuan, Li +9 more
core +1 more source
Recombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii [PDF]
Journal of Kerman University of Medical Sciences, 2015 Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals.
Mehdi Golchin +2 more
doaj
In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans
FEBS Open Bio, EarlyView.In vitro induction of human regulatory T cells using standard protocols generates suppressive, Foxp3‐expressing CD4+ T cells independently of TGF‐β supplementation and donor age. Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis.
Lynn Muller +3 more
wiley +1 more source