Results 81 to 90 of about 574,921 (308)

Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile

Frontiers in Microbiology, 2018
Clostridioides difficile is the causative bacterium in 15–20% of all antibiotic associated diarrheas. The symptoms associated with C. difficile infection (CDI) are primarily induced by the two large exotoxins TcdA and TcdB. Both toxins enter target cells
Viola Fühner, Philip Alexander Heine, Saskia Helmsing, Sebastian Goy, Jasmin Heidepriem, Felix F. Loeffler, Stefan Dübel, Ralf Gerhard, Michael Hust   +8 more
doaj   +1 more source

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen. [PDF]

, 2001
We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein
Oscar Bruña-Romero, Gloria González-Aseguinolaza, Ruth S. Nussenzweig, Tsuji, M, González-Aseguinolaza, G, Julius C. R. Hafalla, Bruña-Romero, O, Hafalla, JC, Nussenzweig, RS, Moriya Tsuji   +9 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Immunolocalization of SP22 (Park7) on the human sperm membrane is indicative of live birth

Reproduction and Fertility
With the decline in sperm quality over the recent decades, there is little doubt that the decreased efficiency of spermatogenesis in men reflects their increasing susceptibility to environmental and pharmaceutical exposures that can compromise male ...
Gary R Klinefelter, Michael P Diamond, Stephen A Krawetz   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Production of Chicken Egg Yolk Immuno-globulin (IgY) against Recombinant TolC Pro-tein From Shigella flexneri Bacteria [PDF]

Iranian South Medical Journal
Background: Growing antibiotic resistance is a global concern for the treatment of shigellosis. The development of efflux pumps such as the AcrAB-TolC pump has increased antibiotic resistance in Shigella flexneri strains.
Hossein Samiei-Abianeh, Shahram Nazarian, Amir Resaei, Nahal Hadi, Kosar Kayvan shokooh   +4 more
doaj  

Vaccination contre la souche influenza PR8 à l'aide d'un vaccin recombinant SG33-M2e [PDF]

, 2010
Cette étude présente un essai d’immunisation à l’aide d’un vaccin recombinant poxviral. Le processus de fabrication du vaccin est basé sur la fusion protéique entre l’antigène considéré et une protéine d’enveloppe du poxvirus.
Boyer, Guillaume
core  

Anti-Severe Acute Respiratory Syndrome Coronavirus Spike Antibodies Trigger Infection of Human Immune Cells via a pH-and Cysteine Protease-Independent Fc gamma R Pathway [PDF]

, 2011
Copyright © 2011, American Society for Microbiology. All Rights Reserved.Public health measures successfully contained outbreaks of the severe acute respiratory syndrome coronavirus (SARS-CoV) infection.
Cheung, Chung y, Marc Daëron, Li, PH, Altmeyer, Ralf, Ping H. Li, Leung, Hiu L, Escriou, N, Dutry, Isabelle, Ming S. Yip, Kien, Francois, Cheung, CY, Dutry, I, Li, Ping H, Benoît Callendret, Nal, B, Chung Y. Cheung, Altmeyer, R, Peiris, J S Malik, Ralf Altmeyer, Bruzzone, R, Jaume, Martial, Martial Jaume, Nicolas Escriou, Callendret, B, Isabelle Dutry, Escriou, Nicolas, Beatrice Nal, Kien, F, Francois Kien, Jaume, M, Yip, MS, Bruzzone, Roberto, J. S. Malik Peiris, Yip, Ming S, Daëron, Marc, Roberto Bruzzone, Hiu L. Leung, Leung, HL, Peiris, JSM, Nal, Beatrice, Daeron, M   +40 more
core   +1 more source