Results 51 to 60 of about 1,379,490 (255)

Method for obtaining recombinant antibodies produced by a cell line transduced with recombinant adenoviruses

Тонкие химические технологии, 2023
Objectives. To develop a technology for obtaining recombinant antibodies in a suspension culture of human HEK293 cells using transduction with recombinant adenovirus serotype 5 (rAd5) carrying genes expressing heavy and light chains of antibodies on the ...
E. S. Sedova, D. N. Shcherbinin, A. S. Bandelyuk, L. V. Verkhovskaya, N. Yu. Viskova, E. D. Avdonina, V. V. Prokofiev, E. I. Ryabova, I. B. Esmagambetov, K. A. Pervoykina, E. A. Bogacheva, A. A. Lysenko, M. M. Shmarov   +12 more
doaj   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus

Журнал микробиологии, эпидемиологии и иммунобиологии
Introduction. Respiratory syncytial virus (RSV) is the most common pathogen causing lower respiratory tract infections in children. RSV also poses a serious threat to the elderly and immunocompromised patients.
Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov   +9 more
doaj   +1 more source

The alpha/B.1.1.7 SARS-CoV-2 variant exhibits significantly higher affinity for ACE-2 and requires lower inoculation doses to cause disease in K18-hACE2 mice

eLife, 2021
The alpha/B.1.1.7 SARS-CoV-2 lineage emerged in autumn 2020 in the United Kingdom and transmitted rapidly until winter 2021 when it was responsible for most new COVID-19 cases in many European countries.
Rafael Bayarri-Olmos, Laust Bruun Johnsen, Manja Idorn, Line S Reinert, Anne Rosbjerg, Søren Vang, Cecilie Bo Hansen, Charlotte Helgstrand, Jais Rose Bjelke, Theresa Bak-Thomsen, Søren R Paludan, Peter Garred, Mikkel-Ole Skjoedt   +12 more
doaj   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

Isolation and Evaluation of Specific Human Recombinant Antibodies from a Phage Display Library against HER3 Cancer Signaling Antigen

Middle East Journal of Cancer, 2014
Background: The human epidermal growth factor receptor family comprises four homologous members: EGFR (ErbB1), ErbB2 (HER2), ErbB3 (HER3) and ErbB4 (HER4).This family plays an important role in the signaling pathway and cell proliferation.
Foroogh Nejatollahi, Samira Silakhori, Bahareh Moazen   +2 more
doaj  

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen. [PDF]

, 2001
We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein
Oscar Bruña-Romero, Gloria González-Aseguinolaza, Ruth S. Nussenzweig, Tsuji, M, González-Aseguinolaza, G, Julius C. R. Hafalla, Bruña-Romero, O, Hafalla, JC, Nussenzweig, RS, Moriya Tsuji   +9 more
core   +1 more source

Characterization of an intracellular humanized single-chain antibody to matrix protein (M1) of H5N1 virus.

PLoS ONE, 2022
We developed a human intracellular antibody based on the M1 protein from avian influenza virus H5N1 (A/meerkat/Shanghai/SH-1/2012) and then characterized the properties of this antibody.
He Sun, Guangmou Wu, Jiyuan Zhang, Yu Wang, Yue Qiu, Hongyang Man, Guoli Zhang, Zehong Li, Yuhuan Yue, Yuan Tian   +9 more
doaj   +1 more source