Results 31 to 40 of about 574,921 (308)

Recombinant antibodies and their use in biosensors [PDF]

Analytical and Bioanalytical Chemistry, 2011
Inexpensive, noninvasive immunoassays can be used to quickly detect disease in humans. Immunoassay sensitivity and specificity are decidedly dependent upon high-affinity, antigen-specific antibodies. Antibodies are produced biologically. As such, antibody quality and suitability for use in immunoassays cannot be readily determined or controlled by ...
Xiangqun, Zeng, Zhihong, Shen, Ray, Mernaugh   +2 more
openaire   +2 more sources

Heterologous prime-boost-boost immunisation of Chinese cynomolgus macaques using DNA and recombinant poxvirus vectors expressing HIV-1 virus-like particles [PDF]

, 2011
Background: There is renewed interest in the development of poxvirus vector-based HIV vaccines due to the protective effect observed with repeated recombinant canarypox priming with gp120 boosting in the recent Thai placebo-controlled trial.
Bridge, S., Skinner, Michael A., Anson, Donald, Dennis, M., Simon H Bridge, Dowall, S., Blanchard, Tom J., Getty, B., Stewart, James, Dennis, Mike J., Dowall, SD, Donald S Anson, Anson, D., Dennis, MJ, Bridge, Simon H., Dowall, Stuart D., Blanchard, Thomas, Stewart, JP, Blanchard, T., Skinner, M., Stewart, James P., James P Stewart, Mike J Dennis, Getty, B, Michael A Skinner, Bridge, SH, Bridge, Simon, Sharpe, Sally, Sharpe, SA, Skinner, MA, Getty, Brian, Brian Getty, Dennis, Mike, Blanchard, Tom, Sharpe, Sally A., Anson, DS, Sally A Sharpe, Skinner, Michael, Stuart D Dowall, Tom J Blanchard, Blanchard, TJ, Anson, Donald S., Stewart, J., Sharpe, S., Dowall, Stuart   +44 more
core   +1 more source

Recombinant renewable polyclonal antibodies [PDF]

mAbs, 2015
Only a small fraction of the antibodies in a traditional polyclonal antibody mixture recognize the target of interest, frequently resulting in undesirable polyreactivity. Here, we show that high-quality recombinant polyclonals, in which hundreds of different antibodies are all directed toward a target of interest, can be easily generated in vitro by ...
FERRARA, FORTUNATO, D'ANGELO, SARA, GAIOTTO, TIZIANO, Naranjo, Leslie, Tian, Hongzhao, Gräslund, Susanne, Dobrovetsky, Elena, Hraber, Peter, Lund Johansen, Fridtjof, Saragozza, Silvia, SBLATTERO, DANIELE, Kiss, Csaba, Bradbury, Andrew R. M.   +12 more
openaire   +3 more sources

Antibody degradation in tobacco plants: a predominantly apoplastic process. [PDF]

, 2011
BACKGROUND: Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem.
Hehle, VK, Drake Pascal M, Verena K Hehle, van Dolleweerd, CJ, Matthew J Paul, Drake, PM, Craig J van Dolleweerd, Julian KC Ma, van Dolleweerd Craig J, Ma, JK, Hehle Verena K, Ma Julian KC, Paul Matthew J, Paul, MJ, Pascal M Drake   +14 more
core   +1 more source

Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli [PDF]

Iranian Journal of Basic Medical Sciences, 2016
Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep.
Khadijeh Nasiri, Saeed Zibaee, Mohammadreza Nassiri, Mojtaba Tahmoorespur, Alireza Haghparast   +4 more
doaj   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen, Mauch, Ludwig, Walter, Michael, Northemann, Wolfgang, Berg, Heike   +4 more
core   +1 more source

Phage Displayed Domain Antibodies (dAb) for Detection of Allergenic Pistachio Proteins in Foods

Foods, 2020
Pistachio nuts (Pistacia vera) have been consumed by past and present-day civilizations because of their organoleptic characteristics and potential health benefits.
Raquel Madrid, Aina García-García, Isabel González, Rosario Martín, Teresa García   +4 more
doaj   +1 more source

Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans

, 2013
Rabies kills many people throughout the developing world every year. The murine monoclonal antibody (mAb) 62-71-3 was recently identified for its potential application in rabies postexposure prophylaxis (PEP).
Both, L, Bulmer-Thomas, B., Banyard, AC, Selden, D., Altmann, F, Craig van Dolleweerd (406086), David Selden (4911475), Selden, D, Ashley C Banyard (16267520), Both, L., Banyard, A.C., Edward Wright (4468198), Bulmer-Thomas, B, Fooks, AR, Friedrich Altmann (33138), Wright, E, van Dolleweerd, C., Fooks, A.R., Ma, J.K.C., Julian K C Ma (16267529), Leonard Both (16267517), van Dolleweerd, C, Ma, JK, Wright, E., Altmann, F., Anthony R Fooks (16267526), Bianca Bulmer-Thomas (16267523)   +26 more
core   +1 more source

Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone

Virology Journal, 2010
Background Hepatitis C virus (HCV) infection is a major public health problem with more than 170 million cases of chronic infections worldwide. There is no protective vaccine currently available for HCV, therefore the development of novel strategy to ...
Clementi Massimo, Burioni Roberto, Liu Gerald, Prabhu Ramesh, Gunduz Feyza, Poat Bret, Hazari Sidhartha, Chandra Partha K, Garry Robert F, Dash Srikanta   +9 more
doaj   +1 more source

EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT [PDF]

Veterinary Medical Journal - Giza, 2000
The recombinant protein (r1448) in crude lysate of insect cells infected with a previously devcl-oped recombinant baculovirus, expressing two structural protcins, the nucleocapsid protein (p14) and the envelope glycoprotein (gp48) of the NADL strain of ...
SAMIRA SAID, M EL-SABBAGH, A EL-KHOLY, H GHALY   +3 more
doaj   +1 more source