Results 31 to 40 of about 1,379,490 (255)

Antibody degradation in tobacco plants: a predominantly apoplastic process. [PDF]

, 2011
BACKGROUND: Interest in using plants for production of recombinant proteins such as monoclonal antibodies is growing, but proteolytic degradation, leading to a loss of functionality and complications in downstream purification, is still a serious problem.
Hehle, VK, Drake Pascal M, Verena K Hehle, van Dolleweerd, CJ, Matthew J Paul, Drake, PM, Craig J van Dolleweerd, Julian KC Ma, van Dolleweerd Craig J, Ma, JK, Hehle Verena K, Ma Julian KC, Paul Matthew J, Paul, MJ, Pascal M Drake   +14 more
core   +1 more source

Phage Displayed Domain Antibodies (dAb) for Detection of Allergenic Pistachio Proteins in Foods

Foods, 2020
Pistachio nuts (Pistacia vera) have been consumed by past and present-day civilizations because of their organoleptic characteristics and potential health benefits.
Raquel Madrid, Aina García-García, Isabel González, Rosario Martín, Teresa García   +4 more
doaj   +1 more source

Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor

, 2022
S.161-172Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG-secreting BY-2 cells in a 200 L orbitally shaken ...
Rasche, S., Raven, N., Schillberg, S.
core   +1 more source

Unraveling the impact of SARS-CoV-2 mutations on immunity: insights from innate immune recognition to antibody and T cell responses

Frontiers in Immunology
Throughout the COVID-19 pandemic, the emergence of new viral variants has challenged public health efforts, often evading antibody responses generated by infections and vaccinations. This immune escape has led to waves of breakthrough infections, raising
Rafael Bayarri-Olmos, Rafael Bayarri-Olmos, Adrian Sutta, Adrian Sutta, Anne Rosbjerg, Anne Rosbjerg, Mie Mandal Mortensen, Charlotte Helgstrand, Per Franklin Nielsen, Laura Pérez-Alós, Beatriz González-García, Laust Bruun Johnsen, Finn Matthiesen, Thomas Egebjerg, Cecilie Bo Hansen, Alessandro Sette, Alessandro Sette, Alba Grifoni, Ricardo da Silva Antunes, Peter Garred, Peter Garred   +20 more
doaj   +1 more source

The production of polyclonal, monoclonal and genetically-derived scFv antibody fragments for the detection of the ß-lactam antibiotic, cephalexin, in milk [PDF]

, 2006
This thesis describes the production of antibodies for the detection of cephalexin and their application in ELISA and Biacore ‘real-time’ surface plasmon resonance (SPR)-based biosensor assays.
Darmanin Sheehan, Alfredo
core   +2 more sources

Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone

Virology Journal, 2010
Background Hepatitis C virus (HCV) infection is a major public health problem with more than 170 million cases of chronic infections worldwide. There is no protective vaccine currently available for HCV, therefore the development of novel strategy to ...
Clementi Massimo, Burioni Roberto, Liu Gerald, Prabhu Ramesh, Gunduz Feyza, Poat Bret, Hazari Sidhartha, Chandra Partha K, Garry Robert F, Dash Srikanta   +9 more
doaj   +1 more source

EVALUAT ION OF A RECOMBINANT ELISA ANTIGEN FOR SERODIAGNOSIS OF BOVINE VIRAL DIARRHEA IN IMMUNE AND INFECTED CATTLE SERA IN EGYPT [PDF]

Veterinary Medical Journal - Giza, 2000
The recombinant protein (r1448) in crude lysate of insect cells infected with a previously devcl-oped recombinant baculovirus, expressing two structural protcins, the nucleocapsid protein (p14) and the envelope glycoprotein (gp48) of the NADL strain of ...
SAMIRA SAID, M EL-SABBAGH, A EL-KHOLY, H GHALY   +3 more
doaj   +1 more source

Shelf-Life Extension of Fc-Fused Single Chain Fragment Variable Antibodies by Lyophilization

Frontiers in Cellular and Infection Microbiology, 2021
Generation of sequence defined antibodies from universal libraries by phage display has been established over the past three decades as a robust method to cope with the increasing market demand in therapy, diagnostics and research.
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Esther Veronika Wenzel, Jan-Hendrik Grosch, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Giulio Russo, Stefan Dübel   +13 more
doaj   +1 more source

IgG antibody responses to Plasmodium falciparum merozoite antigens in Kenyan children have a short half-life. [PDF]

, 2007
BACKGROUND: Data suggest that antibody responses to malaria parasites merozoite antigens are generally short-lived and this has implications for serological studies and malaria vaccine designs.
Conway, David J, Conway David J, Marsh Kevin, Kevin Marsh, Lanar David E, Kinyanjui, Samson M, Lanar, David E, David E Lanar, David J Conway, Kinyanjui Samson M, Marsh, Kevin, Samson M Kinyanjui   +11 more
core   +1 more source

Investigating Alternative Container Formats for Lyophilization of Biological Materials Using Diphtheria Antitoxin Monoclonal Antibody as a Model Molecule

Pharmaceutics, 2021
When preparing biological reference materials, the stability of the lyophilized product is critical for long-term storage, particularly in order to meet WHO International Standards, which are not assigned expiry dates but are expected to be in use for ...
Kiran P. Malik, Chinwe Duru, Paul Stickings, Esther Veronika Wenzel, Michael Hust, Paul Matejtschuk   +5 more
doaj   +1 more source