Results 21 to 30 of about 574,921 (308)

Generation of an Ovomucoid-Immune scFv Library for the Development of Novel Immunoassays in Hen’s Egg Detection

Foods, 2023
Hen’s egg allergy is the second most common food allergy among infants and young children. The possible presence of undeclared eggs in foods poses a significant risk to sensitized individuals. Therefore, reliable egg allergen detection methods are needed
Santiago Rodríguez, Aina García-García, Eduardo Garcia-Calvo, Vanesa Esteban, Carlos Pastor-Vargas, Araceli Díaz-Perales, Teresa García, Rosario Martín   +7 more
doaj   +1 more source

Experimental and In Silico Modelling Analyses of the Gene Expression Pathway for Recombinant Antibody and By-Product Production in NS0 Cell Lines [PDF]

, 2012
Monoclonal antibodies are commercially important, high value biotherapeutic drugs used in the treatment of a variety of diseases. These complex molecules consist of two heavy chain and two light chain polypeptides covalently linked by disulphide bonds ...
Martin, Elaine B., C Mark Smales, Sarah K. Spurgeon (128819), Mead, Emma J., Emma J. Mead (128810), Smales, C. Mark, Chiverton, Lesley M., Smales, Christopher Mark, Elaine B. Martin (128823), C. Mark Smales (128831), Spurgeon Sarah K., Spurgeon, Sarah K., Montague, Gary A.; id_orcid, Montague, Gary A, Smales C. Mark, Montague, Gary A., von der Haar Tobias, Smales, C Mark, Emma J Mead, Sarah K Spurgeon, Gary A Montague, Martin Elaine B., Gary A. Montague (128826), von der Haar, Tobias, Mead, Emma J, Lesley M. Chiverton (128814), Chiverton Lesley M., Elaine B Martin, Tobias von der Haar, Tobias von der Haar (80575), Mead Emma J., Montague Gary A., Lesley M Chiverton   +32 more
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Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Phase I clinical study of the recombinant antibody toxin scFv(FRP5)-ETA specific for the ErbB2/HER2 receptor in patients with advanced solid malignomas [PDF]

, 2005
INTRODUCTION: ScFv(FRP5)-ETA is a recombinant antibody toxin with binding specificity for ErbB2 (HER2). It consists of an N-terminal single-chain antibody fragment (scFv), genetically linked to truncated Pseudomonas exotoxin A (ETA).
Wels, Winfried, Sebastian Harder, Sibylle Loibl, Christian Cimpoiasu, Akin Atmaca, Cimpoiasu, C, Elke Jäger, Alexander Knuth, Dirk Jäger, Minckwitz, Gunter von, Neumann, A M, Harder, S, Cimpoiasu, Christian, Antje Neumann, Hövelmann, S, Winfried S Wels, Atmaca, Akin, Knuth, Alexander, Sascha Hövelmann, Abera, A, Abera, Aklil, Maurer, Alexander B., Gunter von Minckwitz, Aklil Abera, Al-Batran, S E, Knuth, A L, Loibl, S G, Hövelmann, Sascha, Jäger, Elke, Jäger, Dirk, Salah-Eddin Al-Batran, Alexander B Maurer, Loibl, Sibylle, Maurer, A B, Manfred Kaufmann, Wels, W S, von Minckwitz, G, Neumann, Antje, Jäger, D, Jäger, E, Atmaca, A, Kaufmann, M, Batran, Salah-Eddin al-, Kaufmann, Manfred, Harder, Sebastian   +44 more
core   +1 more source

Anti-VDAC3 recombinant antibody decreased human sperm motility and membrane integrity: A potential spermicide for contraception

Asian Pacific Journal of Reproduction, 2017
Objective: To express recombinant protein that comprises an important fragment of human sperm specific voltage dependent anion channel 3 (VDAC3) protein as a potential molecule for generation of antibody, which can affect sperm function, aiming at ...
Asmarinah, Tri Panjiasih Susmiarsih, Amalia Shari, Putri Ratri, Dwi Ari Pujianto, Endang Winiati Bachtiar   +5 more
doaj   +1 more source

Virus-neutralizing monoclonal antibody expressed in milk of transgenic mice provides full protection against virus-induced encephalitis [PDF]

, 2001
Neutralizing antibodies represent a major host defense mechanism against viral infections. In mammals, passive immunity is provided by neutralizing antibodies passed to the offspring via the placenta or the milk as immunoglobulin G and secreted ...
Pewe, L, John Webster, Whitelaw, C B, Andreas F. Kolb, Perlman, S, Siddell, S G, Stanley Perlman, C. Bruce A. Whitelaw, Stuart G. Siddell, Webster, J, Kolb, A F, Lecia Pewe   +11 more
core   +1 more source

Microarray of Recombinant Antibodies Using a Streptavidin Sensor Surface Self-Assembled onto a Gold Layer

BioTechniques, 2003
We have developed a sensitive method for the detection of recombinant antibody-antigen interactions in a microarray format. The biochip sensor platform used in this study is based on an oriented streptavidin monolayer that provides a biological interface
P. Pavlickova, A. Knappik, D. Kambhampati, F. Ortigao, H. Hug   +4 more
doaj   +1 more source

A Humoral Immune Response Alters the Distribution of Enzyme Replacement Therapy in Murine Mucopolysaccharidosis Type I

Molecular Therapy: Methods & Clinical Development, 2018
Antibodies against recombinant proteins can significantly reduce their effectiveness in unanticipated ways. We evaluated the humoral response of mice with the lysosomal storage disease mucopolysaccharidosis type I treated with weekly intravenous ...
Steven Q. Le, Shih-hsin Kan, Don Clarke, Valentina Sanghez, Martin Egeland, Kristen N. Vondrak, Terence M. Doherty, Moin U. Vera, Michelina Iacovino, Jonathan D. Cooper, Mark S. Sands, Patricia I. Dickson   +11 more
doaj   +1 more source

Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system [PDF]

, 2002
The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor ...
Daly, Stephen J., Killard, Anthony J., Dunne, Lynsey, O\u27Kennedy, Richard, Dillon, Paul P., Manning, Bernadette M., O'Kennedy, Richard   +6 more
core   +1 more source

A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M

Fish and Shellfish Immunology Reports, 2023
The development and validation of the recombinant 9E1 monoclonal antibody against channel catfish IgM is described. The variable heavy and light chain domains of the 9E1 hybridoma were cloned into murine IgG1 and IgK expression vectors.
Miles D. Lange, Emily M. Churchman, Allison L. Wise, Timothy J. Bruce   +3 more
doaj   +1 more source