Results 11 to 20 of about 574,921 (308)

CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing

Communications Biology, 2021
Kelil, Gallo et al. develop an antibody discovery strategy, termed CellectSeq, for targeting integral membrane proteins on native cells. This strategy uses motif-based scoring and sequencing error-filtering algorithms to identify antibodies with high ...
Abdellali Kelil, Eugenio Gallo, Sunandan Banerjee, Jarrett J. Adams, Sachdev S. Sidhu   +4 more
doaj   +1 more source

Demystified… recombinant antibodies [PDF]

Journal of Clinical Pathology, 2004
Recombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanised antibodies is given, together with details of the generation of antibody fragments—for example, single chain Fv fragments.
Smith, K.A., Nelson, Paul N., Warren, Phil, Astley, S.J., Murray, Paul G., Greenman, J.   +5 more
openaire   +4 more sources

Antibody response to Newcastle disease virus recombinant fusion protein in post-vaccinated laying hens

Journal of the Indonesian Tropical Animal Agriculture, 2023
This research was aimed to analyze antibody response in laying hens post vaccinated by Newcastle Disease Virus (NDV) recombinat Fusion (F) protein which has been succesfully expressed from the F gene of local isolates of NDV from Kulon Progo strain (0663/
J. Jawad, R. W. Astuti, A. Haryanto, N. Wijayanti   +3 more
doaj   +1 more source

A strong case for third-party testing

eLife, 2023
A strategy to identify high-quality commercially available antibodies for research reveals extensive use of non-specific antibodies and offers solutions for future large-scale testing.
Fridtjof Lund-Johansen
doaj   +1 more source

Recombinant Antibody Technology [PDF]

ILAR Journal, 1995
36:801-803. Smith, D.B., and K.S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40. Snapper, CM., and J.J. Mond. 1993. Towards a comprehensive view of immunoglobulin class switching. Immunol. Today 14:15-17. Snorasse, T., B. Flamand, G. De Becker, H. Bazin, F.
Alexander E., Karn, Christopher W., Bell, Tina F., Chin   +2 more
openaire   +2 more sources

Isolation and characterization of full-length genes encoding the anti-human CD45 antibody from the hybridoma cell line 16E8-F2

Ho Chi Minh City Open University Journal of Science - Engineering and Technology, 2022
Though the hybridoma technology has been widely applied in the production of monoclonal antibodies, it has existed some disadvantages including low yield and genetic instability. Therefore, an alternative approach should be taken into account.
Nguyen Quoc Huy, Ta Huong Giang, Nguyen Dang Quan   +2 more
doaj   +1 more source

Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination

Journal of the Indonesian Tropical Animal Agriculture, 2020
This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody ...
R.W. Astuti, N. Wijayanti, A. Haryanto
doaj   +1 more source

Prediction of Clinical Requirement of Tocilizumab Injection in COVID-19 Patients with High Chest CT Severity Score- A Retrospective Analysis [PDF]

Journal of Clinical and Diagnostic Research, 2021
Introduction: The ongoing Coronavirus Disease 2019 (COVID19) pandemic has spread rapidly across the globe. Tocilizumab is a recombinant monoclonal antibody to Interleukin-6 (IL-6) receptor. An increasing number of studies across the world is reporting
MEGHA SHETH, YASHPAL RANA, DINESH PATEL, ANSHUL GHAI, SAMIR PATEL, MILIN GARACHH, PINKESH SHAH, KRUTIKA PATEL   +7 more
doaj   +1 more source

Cell growth inhibition and apoptotic effects of a specific anti-RTFscFv antibody on prostate cancer, but not glioblastoma, cells [version 1; referees: 2 approved, 1 approved with reservations]

F1000Research, 2017
Background: Single chain antibody (scFv) has shown interesting results in cancer immunotargeting approaches, due to its advantages over monoclonal antibodies.
Foroogh Nejatollahi, Payam Bayat, Bahareh Moazen   +2 more
doaj   +1 more source

Development of a Novel Anti-EpCAM Monoclonal Antibody for Various Applications

Antibodies, 2022
The epithelial cell adhesion molecule (EpCAM) is a cell surface glycoprotein, which is widely expressed on normal and cancer cells. EpCAM is involved in cell adhesion, proliferation, survival, stemness, and tumorigenesis.
Guanjie Li, Hiroyuki Suzuki, Teizo Asano, Tomohiro Tanaka, Hiroyoshi Suzuki, Mika K. Kaneko, Yukinari Kato   +6 more
doaj   +1 more source