Results 231 to 240 of about 6,607,627 (360)

Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli. [PDF]

STAR Protoc
Dahms SO, Wieland AC, Brandstetter H, Dall E.   +3 more
europepmc   +1 more source

Robust Recombinant Expression of Human Placental Ribonuclease Inhibitor in Insect Cells. [PDF]

Biomolecules, 2022
Flachner B, Dobi K, Benedek A, Cseh S, Lőrincz Z, Hajdú I.   +5 more
europepmc   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

Escherichia coli recombinant expression of SARS-CoV-2 protein fragments. [PDF]

Microb Cell Fact, 2022
McGuire BE, Mela JE, Thompson VC, Cucksey LR, Stevens CE, McWhinnie RL, Winkler DFH, Pelech S, Nano FE.   +8 more
europepmc   +1 more source

Functional Analysis of Ligand‐Gated Chloride Channels in a Cnidarian Sheds Light on the Evolution of Inhibitory Signaling

Advanced Science, EarlyView.
We uncover a large variety of putative inhibitory ligand‐gated ion channels (LGICs) in the phylum Cnidaria, the sister group to all bilaterian animals. Phylogenetic analysis suggests a complex evolutionary history of inhibitory LGICs with diverse neurotransmitter ligands.
Abhilasha Ojha, Linda Kloss, Juan D. Montenegro, Simone Albani, Audrey Ortega‐Ramírez, Mihaela Raycheva, Sylvia Joussen, Sabrina Kaul, Michèle Bachmann, Lisa Huf, Günther Schmalzing, Alison G. Cole, Ulrich Technau, Stefan Gründer   +13 more
wiley   +1 more source

Recombinant Expression and Purification of the Cyanobacterial Chaperone HtpG from <i>Synechococcus elongatus</i> PCC 7942. [PDF]

Methods Protoc
Jiang L, Boyenle ID, Delaeter N, Liu Y.
europepmc   +1 more source

Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis. [PDF]

Microb Cell Fact, 2023
Haeger G, Wirges J, Tanzmann N, Oyen S, Jolmes T, Jaeger KE, Schörken U, Bongaerts J, Siegert P.   +8 more
europepmc   +1 more source

Adipocyte‐Derived Leptolin Enhances Energy Expenditure and Prevents Obesity

Advanced Science, EarlyView.
We identified a novel adipokine, which we named leptolin. In humans, leptolin levels in white adipose tissue were positively correlated with exercise and negatively associated with body mass index. We observed elevated leptolin in serum from athletes and lower leptolin in serum from obese individuals.
Jiarui Liu, Bingwei Wang, Zhijie Su, Xiaoxv Han, Miao He, Yun Zhao, Yujia Hou, Daotong Li, Weiguang Zhang, Lihua Qin, Ke Wang, Yanchun Li, Yi Yan, Siwang Yu, Xiaoshuai Huang, Tairan Yuwen, Jianyuan Luo, Ruimao Zheng   +17 more
wiley   +1 more source

Recombinant Expression and Bioprocess Optimization of Priestia megaterium α-Amylase and Its Impact on Dough Fermentation Efficiency. [PDF]

Chem Biodivers
Erdem A, Akbulut K, Türker M, Binay B.
europepmc   +1 more source

Development of Transient Recombinant Expression and Affinity Chromatography Systems for Human Fibrinogen. [PDF]

Int J Mol Sci, 2022
Popovic G, Kirby NC, Dement TC, Peterson KM, Daub CE, Belcher HA, Guthold M, Offenbacher AR, Hudson NE.   +8 more
europepmc   +1 more source