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Expression and isolation of recombinant tau
2017In this chapter, we describe methods for the purification of both untagged and polyhistidine-tagged tau protein. These protocols utilize a bacterial expression system to produce the tau isoform of interest, followed by heat treatment and column chromatography to separate tau from impurities.
Rebecca L, Best +6 more
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The expression of recombinant proteins in yeasts
Current Opinion in Biotechnology, 1996The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically ...
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Nature Methods, 2007
A new method for expressing functional RNA molecules in vivo opens a route to easy RNA sample preparation, and establishes a set of tools for studies of RNA structure, interactions and function in vitro and in vivo.
Dunham, Christine M., Conn, Graeme L.
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A new method for expressing functional RNA molecules in vivo opens a route to easy RNA sample preparation, and establishes a set of tools for studies of RNA structure, interactions and function in vitro and in vivo.
Dunham, Christine M., Conn, Graeme L.
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Expression of recombinant myeloperoxidase using a baculovirus expression system
Biochemical and Biophysical Research Communications, 1992Myeloperoxidase (MPO) is a glycosylated heme-containing enzyme present in the azurophilic granules of normal human polymorphonuclear neutrophils. This enzyme plays a major role in the microbicidal activity of the host defense system by catalyzing the formation of the potent oxidant, hypochlorous acid.
K L, Taylor, D J, Uhlinger, J M, Kinkade
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Effects of Oxygen on Recombinant Protein Expression
Biotechnology Progress, 1998Efforts to increase cell growth and protein yields need to be complemented by the maintenance of the quality of the protein produced. Elevated oxygen pressure or rapid increases in oxygen content can cause oxidative stress within the cells, leading to oxidation of specific proteins and nucleotide sequences. In addition, transient or steady-state anoxic
J O, Konz, J, King, C L, Cooney
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Recombinant protein expression
2013This chapter zooms in on technical issues related to the systems commonly used for recombinant protein production and discusses the advantages and disadvantages of each. Among these expression systems are E. coli, Lactobacillus lactis, Saccharomyces cerevisiae, Pichia pastoris, insect larval cells, mammalian cells, and cell-free systems.
Michael Harrison, Michael J. McPherson
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Recombinant expression systems in the pharmaceutical industry
Applied Microbiology and Biotechnology, 2004In terms of downstream processing efficiency, secretory expression systems offer potential advantages for the production of recombinant proteins, compared with inclusion body forming cytosolic systems. However, for high-volume therapeutics like insulin, the product yields of the majority of the potentially available secretory systems is not yet fully ...
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Optimising expression and extraction of recombinant proteins in plants
Frontiers in Plant Science, 2022Mark T. Young
exaly