Results 321 to 330 of about 6,607,627 (360)
Some of the next articles are maybe not open access.
Recombinant expression systems in the pharmaceutical industry
Applied Microbiology and Biotechnology, 2004In terms of downstream processing efficiency, secretory expression systems offer potential advantages for the production of recombinant proteins, compared with inclusion body forming cytosolic systems. However, for high-volume therapeutics like insulin, the product yields of the majority of the potentially available secretory systems is not yet fully ...
openaire +2 more sources
Recombinant Expression Systems for Allergen Vaccines
Inflammation & Allergy-Drug Targets, 2006Allergen immunotherapy of future is likely to be based on allergy vaccines that contain engineered allergens modified to abolish or substantially reduce their IgE-binding activity in order to remove the risk of unwanted anaphylactic responses. The development of efficient systems for the production of recombinant allergens in sufficient quantities is ...
Mohan B, Singh, Prem L, Bhalla
openaire +2 more sources
Optimal expression condition of recombinant RAP
Journal of Huazhong University of Science and Technology, 2007In order to construct the expression recombinant of human receptor associated protein (RAP), optimize its expression condition and obtain the recombinant protein after expression with high efficiency, two prokaryotic expression vectors-pT7-PL and pET-28a(+) were used to construct the expression recombinant containing RAP cDNA, and the expression ...
Jie, Zhang +5 more
openaire +2 more sources
Expression and purification of a recombinant enantioselective amidase
Protein Expression and Purification, 2005Microbacterium sp. AJ115 metabolises a wide range of nitriles using the two-step nitrile hydratase/amidase pathway. In this study, the amidase gene of Microbacterium sp. AJ115 has been inserted into the pCal-n-EK expression vector and expressed in Escherichia coli BL21(DE3)pLysS. The expressed protein is active in E.
John P, Doran +4 more
openaire +2 more sources
Expression Systems for the Production of Recombinant Pharmaceuticals
BioDrugs, 2004The new generation of biological products are largely the result of genetic engineering. The qualitative and quantitative demand for recombinant proteins is steadily increasing. Molecular biologists are constantly challenged by the need to improve and optimise the existing expression systems, and also develop novel approaches to face the demands of ...
openaire +2 more sources
Understanding recombinant expression of membrane proteins
Current Opinion in Biotechnology, 2006Over the past 15 years, numerous reports have been published on the recombinant expression of integral membrane proteins. Some proteins accumulate in the membrane to high levels, whereas other often closely related proteins are barely detected. Understanding the underlying reasons for this variation has proven difficult.
openaire +2 more sources
ADAMTS7: Recombinant Protein Expression and Purification
2019ADAMTS7 is a secreted protease that is predominantly expressed in tissues of the cardiovascular system and tendon. Although recent evidence suggests that it may play a role in the etiology of coronary artery disease, its physiological function and substrates are unknown.
openaire +2 more sources