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Effects of Oxygen on Recombinant Protein Expression
Biotechnology Progress, 1998Efforts to increase cell growth and protein yields need to be complemented by the maintenance of the quality of the protein produced. Elevated oxygen pressure or rapid increases in oxygen content can cause oxidative stress within the cells, leading to oxidation of specific proteins and nucleotide sequences. In addition, transient or steady-state anoxic
J O, Konz, J, King, C L, Cooney
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Recombinant protein expression
2013This chapter zooms in on technical issues related to the systems commonly used for recombinant protein production and discusses the advantages and disadvantages of each. Among these expression systems are E. coli, Lactobacillus lactis, Saccharomyces cerevisiae, Pichia pastoris, insect larval cells, mammalian cells, and cell-free systems.
Michael Harrison, Michael J. McPherson
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Protein Expression and Purification, 2019
Hispidalin is a novel antimicrobial peptide isolated from the seeds of Benincasa hispida and is reported to have broad antimicrobial activity against various bacterial and fungal pathogens.
De‐Mei Meng +6 more
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Hispidalin is a novel antimicrobial peptide isolated from the seeds of Benincasa hispida and is reported to have broad antimicrobial activity against various bacterial and fungal pathogens.
De‐Mei Meng +6 more
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Recombinant protein expression in Pichia pastoris
Molecular Biotechnology, 2000The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool.
J M, Cregg +3 more
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Expression and purification of recombinant and native calreticulin
Protein Expression and Purification, 1992Calreticulin is a 60-kDa Ca(2+)-binding protein of the endo(sarco)plasmic reticulum membranes of a variety of cellular systems. The protein binds approximately 25 mol of Ca2+ with low affinity and approximately 1 mol of Ca2+ with high affinity and is believed to be a site for Ca2+ binding/storage in the lumen of the endo(sarco)plasmic reticulum. In the
S, Baksh +3 more
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Transient expression of recombinant proteins in plants
2021Transient protein expression in plant cells is less time consuming than the production of whole transgenic plants. For transient expression, agroinfiltration is a simple and effective method to deliver transgenes into plant cells. After an Agrobacterium infection, recombinant proteins can be produced in plant cells from 3 to 10days. To increase protein
Shohei, Nosaki, Kenji, Miura
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High level expression and purification of recombinant human serum albumin in Pichia pastoris.
Protein Expression and Purification, 2018Human serum albumin (HSA) has been extensively used in a series of clinical care settings for nearly seven decades. However, the broad application of this protein is seriously limited by its short supply.
Wenmiao Zhu +6 more
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Expression of a Recombinant Sheep IgE Gene
Immunological Investigations, 1994An ovine cosmid library was screened with a bovine C epsilon gene probe. IgE and IgA constant region genes (C epsilon and C alpha, respectively) were isolated within a single 26 kb cosmid insert. The C epsilon gene was cloned into an expression vector which contained the rearranged VDJ genomic segment encoding an anti-(4-hydroxy-3-iodo-5-nitrophenyl ...
R A, Clarke, K J, Beh
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Recombinant Protein Expression in Leishmania tarentolae
Molecular Biotechnology, 2009A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested
Giancarlo, Basile, Manuela, Peticca
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Expression of Recombinant ADAMTS in Insect Cells
2010The "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) enzymes are secreted proteinases involved in development, blood clotting and the turnover of extracellular matrix. Manufacturing recombinant enzyme presents quite a challenge due to the presence of disulphide bridges, the large size and modular structure. A sub-group of these
Gavin C, Jones +2 more
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