Results 11 to 20 of about 577,747 (248)

Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases [PDF]

open access: bronzeProtein Engineering, 2003
Expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein.
Brigitte Kühnel   +4 more
openalex   +2 more sources

Therapeutic Fc-fusion proteins: Current analytical strategies.

open access: yesJournal of Separation Science, 2020
Fc-Fusion proteins represent a successful class of biopharmaceutical products, with already 13 drugs approved in the European Union and in the USA as well as 3 biosimilar versions of etanercept.
Bastiaan L. Duivelshof   +6 more
semanticscholar   +1 more source

Fibroblast adhesion to recombinant tropoelastin expressed as a protein A-fusion protein [PDF]

open access: yesBiochemical Journal, 1991
A bovine tropoelastin cDNA encoding exons 15-36 that includes the elastin-receptor binding site was expressed in Escherichia coli as a fusion protein with Protein A from Staphylococcus aureus. After isolation of the fusion protein by affinity chromatography on Ig-Sepharose, the tropoelastin domain was separated from plasmid-pR1T2T-encoded Protein A ...
Robert P. Mecham   +3 more
openaire   +3 more sources

Expression Vectors for Enzyme Restriction- and Ligation-Independent Cloning for Producing Recombinant His-Fusion Proteins [PDF]

open access: greenBiotechnology progress (Print), 2007
Blanca de las Rivas   +3 more
openalex   +2 more sources

DEVELOPMENT OF THE VACCINE BASED ON THE RECOMBINANT ANTIGENS OF PSEUDOMONAS AERUGINOSA

open access: yesЖурнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. The aim is obtaining, investigation and selection of recombinant antigens for inclusion theirs into the against Pseudomonas vaccine. Materials and methods. The genes encoding of the outer  membrane proteins F, L and I and Exotoxin A were synthesized
N. A. Mihailova   +3 more
doaj   +1 more source

Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins.

open access: yesSmall, 2017
Vesicles assembled from folded, globular proteins have potential for functions different from traditional lipid or polymeric vesicles. However, they also present challenges in understanding the assembly process and controlling vesicle properties.
Yeon-Do Jang   +5 more
semanticscholar   +1 more source

Soluble Form Production of Recombinant Human Insulin-Like Growth Factor-1 by NusA Fusion Partner in E. coli

open access: yesTrends in Peptide and Protein Sciences, 2019
Insulin-Like Growth Factor-1 (IGF-1) is a small peptide with 70 amino acids and 7.6 kDa molecular weight that acts as the major mediator of growth hormone. According to the previous studies, recombinant production of human IGF-1 (rhIGF-1) in E.
Sara Hemmati, Javad Ranjbari
doaj   +1 more source

Deglycosylation of proteins for crystallization using recombinant fusion protein glycosidases [PDF]

open access: yesProtein Science, 1996
AbstractObtaining high quality protein crystals remains a rate‐limiting step in the determination of three‐dimensional X‐ray structures. A frequently encountered problem in this respect is the high or heterogeneous carbohydrate content of many eukaryotic proteins.
Christiane Chène   +3 more
openaire   +3 more sources

Targeted Recombinant Fusion Proteins of IFNγ and Mimetic IFNγ with PDGFβR Bicyclic Peptide Inhibits Liver Fibrogenesis In Vivo

open access: yesPLoS ONE, 2014
Hepatic stellate cells (HSCs), following transdifferentiation to myofibroblasts plays a key role in liver fibrosis. Therefore, attempts to attenuate this myofibroblastic phenotype would be a promising therapeutic approach.
Ruchi Bansal   +3 more
semanticscholar   +1 more source

Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast

open access: yesBMC Biotechnology, 2009
Background Expression of recombinant proteins in green algal chloroplast holds substantial promise as a platform for the production of human therapeutic proteins.
Henry Ryan E   +2 more
doaj   +1 more source

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