Results 11 to 20 of about 577,747 (248)
Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases [PDF]
Protein Engineering, 2003 Expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein.
Brigitte Kühnel +4 more
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Therapeutic Fc-fusion proteins: Current analytical strategies.
Journal of Separation Science, 2020 Fc-Fusion proteins represent a successful class of biopharmaceutical products, with already 13 drugs approved in the European Union and in the USA as well as 3 biosimilar versions of etanercept.
Bastiaan L. Duivelshof +6 more
semanticscholar +1 more source
Fibroblast adhesion to recombinant tropoelastin expressed as a protein A-fusion protein [PDF]
Biochemical Journal, 1991 A bovine tropoelastin cDNA encoding exons 15-36 that includes the elastin-receptor binding site was expressed in Escherichia coli as a fusion protein with Protein A from Staphylococcus aureus. After isolation of the fusion protein by affinity chromatography on Ig-Sepharose, the tropoelastin domain was separated from plasmid-pR1T2T-encoded Protein A ...
Robert P. Mecham +3 more
openaire +3 more sources
Expression Vectors for Enzyme Restriction- and Ligation-Independent Cloning for Producing Recombinant His-Fusion Proteins [PDF]
Biotechnology progress (Print), 2007 Blanca de las Rivas +3 more
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DEVELOPMENT OF THE VACCINE BASED ON THE RECOMBINANT ANTIGENS OF PSEUDOMONAS AERUGINOSA
Журнал микробиологии, эпидемиологии и иммунобиологии, 2019 Aim. The aim is obtaining, investigation and selection of recombinant antigens for inclusion theirs into the against Pseudomonas vaccine. Materials and methods. The genes encoding of the outer membrane proteins F, L and I and Exotoxin A were synthesized
N. A. Mihailova +3 more
doaj +1 more source
Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins.
Small, 2017 Vesicles assembled from folded, globular proteins have potential for functions different from traditional lipid or polymeric vesicles. However, they also present challenges in understanding the assembly process and controlling vesicle properties.
Yeon-Do Jang +5 more
semanticscholar +1 more source
Trends in Peptide and Protein Sciences, 2019 Insulin-Like Growth Factor-1 (IGF-1) is a small peptide with 70 amino acids and 7.6 kDa molecular weight that acts as the major mediator of growth hormone. According to the previous studies, recombinant production of human IGF-1 (rhIGF-1) in E.
Sara Hemmati, Javad Ranjbari
doaj +1 more source
Deglycosylation of proteins for crystallization using recombinant fusion protein glycosidases [PDF]
Protein Science, 1996 AbstractObtaining high quality protein crystals remains a rate‐limiting step in the determination of three‐dimensional X‐ray structures. A frequently encountered problem in this respect is the high or heterogeneous carbohydrate content of many eukaryotic proteins.
Christiane Chène +3 more
openaire +3 more sources
PLoS ONE, 2014 Hepatic stellate cells (HSCs), following transdifferentiation to myofibroblasts plays a key role in liver fibrosis. Therefore, attempts to attenuate this myofibroblastic phenotype would be a promising therapeutic approach.
Ruchi Bansal +3 more
semanticscholar +1 more source
BMC Biotechnology, 2009 Background Expression of recombinant proteins in green algal chloroplast holds substantial promise as a platform for the production of human therapeutic proteins.
Henry Ryan E +2 more
doaj +1 more source