Results 11 to 20 of about 577,747 (248)
Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases [PDF]
Expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein.
Brigitte Kühnel+4 more
openalex +2 more sources
Therapeutic Fc-fusion proteins: Current analytical strategies.
Fc-Fusion proteins represent a successful class of biopharmaceutical products, with already 13 drugs approved in the European Union and in the USA as well as 3 biosimilar versions of etanercept.
Bastiaan L. Duivelshof+6 more
semanticscholar +1 more source
Fibroblast adhesion to recombinant tropoelastin expressed as a protein A-fusion protein [PDF]
A bovine tropoelastin cDNA encoding exons 15-36 that includes the elastin-receptor binding site was expressed in Escherichia coli as a fusion protein with Protein A from Staphylococcus aureus. After isolation of the fusion protein by affinity chromatography on Ig-Sepharose, the tropoelastin domain was separated from plasmid-pR1T2T-encoded Protein A ...
Robert P. Mecham+3 more
openaire +3 more sources
Expression Vectors for Enzyme Restriction- and Ligation-Independent Cloning for Producing Recombinant His-Fusion Proteins [PDF]
Blanca de las Rivas+3 more
openalex +2 more sources
DEVELOPMENT OF THE VACCINE BASED ON THE RECOMBINANT ANTIGENS OF PSEUDOMONAS AERUGINOSA
Aim. The aim is obtaining, investigation and selection of recombinant antigens for inclusion theirs into the against Pseudomonas vaccine. Materials and methods. The genes encoding of the outer membrane proteins F, L and I and Exotoxin A were synthesized
N. A. Mihailova+3 more
doaj +1 more source
Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins.
Vesicles assembled from folded, globular proteins have potential for functions different from traditional lipid or polymeric vesicles. However, they also present challenges in understanding the assembly process and controlling vesicle properties.
Yeon-Do Jang+5 more
semanticscholar +1 more source
Insulin-Like Growth Factor-1 (IGF-1) is a small peptide with 70 amino acids and 7.6 kDa molecular weight that acts as the major mediator of growth hormone. According to the previous studies, recombinant production of human IGF-1 (rhIGF-1) in E.
Sara Hemmati, Javad Ranjbari
doaj +1 more source
Deglycosylation of proteins for crystallization using recombinant fusion protein glycosidases [PDF]
AbstractObtaining high quality protein crystals remains a rate‐limiting step in the determination of three‐dimensional X‐ray structures. A frequently encountered problem in this respect is the high or heterogeneous carbohydrate content of many eukaryotic proteins.
Christiane Chène+3 more
openaire +3 more sources
Hepatic stellate cells (HSCs), following transdifferentiation to myofibroblasts plays a key role in liver fibrosis. Therefore, attempts to attenuate this myofibroblastic phenotype would be a promising therapeutic approach.
Ruchi Bansal+3 more
semanticscholar +1 more source
Background Expression of recombinant proteins in green algal chloroplast holds substantial promise as a platform for the production of human therapeutic proteins.
Henry Ryan E+2 more
doaj +1 more source