Results 31 to 40 of about 282,766 (336)
ViralFP: A webserver of viral fusion proteins [PDF]
arXiv, 2021 Viral fusion proteins are attached to the membrane of enveloped viruses (a group that includes Coronaviruses, Dengue, HIV and Influenza) and catalyze fusion between the viral and host membranes, enabling the virus to insert its genetic material into the host cell.
arxiv
Frontiers in Bioengineering and Biotechnology, 2020 Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products. However, some key enzymes involved in biotransformation processes are unstable and cannot be easily expressed in the functional form.
Yunjeong Park +15 more
doaj +1 more source
Obtaining of the recombinant Rhil7-Cbd fusion protein
Faktori eksperimental'noi evolucii organizmiv, 2020 Aim. The purpose was to obtain the recombinant fusion protein based on the human interleukin-7 (rhIL7) and cellulose binding domain (CBD). Methods. The DNA sequences encoding rhIL-7 and CBD were subcloned into the pET24a(+). Vector containing the 6His-tag sequence for further chromatographic purification of the target protein. The cells of E.
D. M. Irodov +5 more
openaire +2 more sources
The recombinant expression and activity detection of MAF-1 fusion protein [PDF]
Scientific Reports, 2015 AbstractThis study establishes the recombinant expression system of MAF-1 (Musca domesticaantifungal peptide-1) and demonstrates the antifungal activity of the expression product and shows the relationship between biological activity and structure. The gene segments on mature peptide part of MAF-1 were cloned, based on the primers designed according to
Jian Liu +5 more
openaire +3 more sources
Electronic Journal of Biotechnology, 2022 Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps.
Pál Salamon +9 more
doaj
Generation of a vector suite for protein solubility screening
Frontiers in Microbiology, 2014 Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea +5 more
doaj +1 more source
Genetic Structures and Conditions of their Expression, which Allow Receiving Native Recombinant Proteins with High Output [PDF]
International Journal of Biomedicine, 2012 We investigated the possibility of obtaining native recombinant amyloidogenic proteins by creating genetic constructs encoding fusion proteins of target proteins with Super Folder Green Fluorescent Protein (sfGFP).
Michael M. Shavlovsky, PhD, ScD¹ +6 more
doaj
Kinetics of protein-DNA interaction: facilitated target location in sequence-dependent potential [PDF]
Biophys. J. 87:4021-4035 (2004), 2004 Recognition and binding of specific sites on DNA by proteins is central for many cellular functions such as transcription, replication, and recombination. In the process of recognition, a protein rapidly searches for its specific site on a long DNA molecule and then strongly binds this site.
arxiv +1 more source
Automated production of recombinant human proteins as resource for proteome research
Proteome Science, 2008 Background An arbitrary set of 96 human proteins was selected and tested to set-up a fully automated protein production strategy, covering all steps from DNA preparation to protein purification and analysis.
Poustka Annemarie +4 more
doaj +1 more source
ExpressInHost: A codon tuning tool for the expression of recombinant proteins in host microorganisms [PDF]
arXiv, 2021 ExpressInHost (https://gitlab.com/a.raguin/expressinhost) is a GTK/C++ based user friendly graphical interface that allows tuning the codon sequence of an mRNA for recombinant protein expression in a host microorganism. Heterologous gene expression is widely implemented in biotechnology companies and academic research laboratories.
arxiv