Results 31 to 40 of about 399,275 (342)

Coexpressing the Signal Peptide of Vip3A and the Trigger Factor of Bacillus thuringiensis Enhances the Production Yield and Solubility of eGFP in Escherichia coli

Frontiers in Microbiology, 2022
Many fusion tags have been developed to improve the expression of recombinant proteins. Besides the translocation of cargo proteins, the signal peptides (SPs) of some secretory proteins, such as the ssTorA and Iasp, have been used as an inclusion body ...
Jianhua Gao, Chunping Ouyang, Juanli Zhao, Yan Han, Qinghua Guo, Xuan Liu, Tianjiao Zhang, Ming Duan, Xingchun Wang, Chao Xu   +9 more
doaj   +1 more source

Antibody Fusion Proteins: Anti-CD22 Recombinant Immunotoxin Moxetumomab Pasudotox [PDF]

Clinical Cancer Research, 2011
Abstract Recombinant immunotoxins are fusion proteins that contain the cytotoxic portion of a protein toxin fused to the Fv portion of an antibody. The Fv binds to an antigen on a target cell and brings the toxin into the cell interior, where it arrests protein synthesis and initiates the apoptotic cascade.
Robert J, Kreitman, Ira, Pastan
openaire   +2 more sources

The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27 kD γ-zein sequence

Frontiers in Bioengineering and Biotechnology, 2014
Naturally-occurring storage proteins such as zeins are used as fusion partners for recombinant proteins because they induce the formation of ectopic storage organelles known as protein bodies (PBs) where the proteins are stabilized by intermolecular ...
Anna eHofbauer, Jenny ePeters, Elsa eArcalis, Thomas eRademacher, Johannes eLampel, Francois eEudes, Alessandro eVitale, Eva eStoger   +7 more
doaj   +1 more source

Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein [PDF]

Journal of Genetic Engineering and Biotechnology, 2021
The management of stray dog population has been of utmost importance due to their overpopulation, increase in dog bites incidence, and rabies. Contraceptive vaccines, a non-surgical alternative to spaying and neutering are viewed as a valuable option for the management of dog population.
Nathaniel Philip Sandam, Dhamodhar Prakash, Prashanth Thimmareddy   +2 more
openaire   +3 more sources

Expression in Escherichia coli of a cloned DNA sequence encoding the pre-S2 region of hepatitis B virus [PDF]

, 1985
A DNA sequence encoding the entire pre-S2 region (amino acids 120-174; serotype ayw) of human hepatitis B virus envelope protein has been inserted into the lacZ gene of the plasmid pSKS105 yielding a recombinant, pWS3.
Acs, George, Christman, Judith K., Kent, Stephen B. H., Neurath, A. Robert, Offensperger, Wolf, Price, Peter, Strick, Nathan, Wahl, Silke   +7 more
core   +1 more source

TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium

Memorias do Instituto Oswaldo Cruz, 2011
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium flagellin (FliC) protein as an ...
Ariane Guglielmi Ariza Camacho, Laís Helena Teixeira, Daniel Youssef Bargieri, Silvia Beatriz Boscardin, Irene da Silva Soares, Ruth Sonntag Nussenzweig, Victor Nussenzweig, Mauricio Martins Rodrigues   +7 more
doaj   +1 more source

Advances in the design and development of oncolytic measles viruses. [PDF]

, 2015
A successful oncolytic virus is one that selectively propagates and destroys cancerous tissue without causing excessive damage to the normal surrounding tissue. Oncolytic measles virus (MV) is one such virus that exhibits this characteristic and thus has
Hutzen, Brian, Raffel, Corey, Studebaker, Adam W   +2 more
core   +1 more source

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

Viruses, 2018
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins.
Won Seok Gwak, Jae Bang Choi, Beom Ku Han, Sung Min Bae, Soo Dong Woo   +4 more
doaj   +1 more source

Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency

Frontiers in Bioengineering and Biotechnology, 2020
Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products. However, some key enzymes involved in biotransformation processes are unstable and cannot be easily expressed in the functional form.
Yunjeong Park, Jonghyeok Shin, Jinkyeong Yang, Hooyeon Kim, Younghun Jung, Hyunseok Oh, Yongjoon Kim, Jaehyeon Hwang, Myeongseo Park, Choongjin Ban, Choongjin Ban, Ki Jun Jeong, Sun-Ki Kim, Dae-Hyuk Kweon, Dae-Hyuk Kweon, Dae-Hyuk Kweon   +15 more
doaj   +1 more source

Generation of a vector suite for protein solubility screening

Frontiers in Microbiology, 2014
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea, Claudia eOrtega, Gonzalo eObal, Pedro eAlzari, Renaud eVincentelli, Pablo Javier Oppezzo   +5 more
doaj   +1 more source