Results 31 to 40 of about 577,747 (248)

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

BMC Biotechnology, 2013
BackgroundThe demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years.
V. Jäger, K. Büssow, A. Wagner, S. Weber, M. Hust, A. Frenzel, T. Schirrmann   +6 more
semanticscholar   +1 more source

Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins

Agronomy, 2023
Soybean seeds show great potential as a safe and cost-effective host for the large-scale production of biopharmaceuticals and industrially important macromolecules.
Jing Yang, Yuanyu Zhang, Guojie Xing, Jia Wei, Lu Niu, Qianqian Zhao, Qinan Cai, Xiaofang Zhong, Xiangdong Yang   +8 more
doaj   +1 more source

Coexpressing the Signal Peptide of Vip3A and the Trigger Factor of Bacillus thuringiensis Enhances the Production Yield and Solubility of eGFP in Escherichia coli

Frontiers in Microbiology, 2022
Many fusion tags have been developed to improve the expression of recombinant proteins. Besides the translocation of cargo proteins, the signal peptides (SPs) of some secretory proteins, such as the ssTorA and Iasp, have been used as an inclusion body ...
Jianhua Gao, Chunping Ouyang, Juanli Zhao, Yan Han, Qinghua Guo, Xuan Liu, Tianjiao Zhang, Ming Duan, Xingchun Wang, Chao Xu   +9 more
doaj   +1 more source

Obtaining of the recombinant Rhil7-Cbd fusion protein

Faktori eksperimental'noi evolucii organizmiv, 2020
Aim. The purpose was to obtain the recombinant fusion protein based on the human interleukin-7 (rhIL7) and cellulose binding domain (CBD). Methods. The DNA sequences encoding rhIL-7 and CBD were subcloned into the pET24a(+). Vector containing the 6His-tag sequence for further chromatographic purification of the target protein. The cells of E.
D. M. Irodov, O. B. Gorbatiuk, M. V. Koval’chuk, O. V. Okunev, V. A. Kordium, M. O. Usenko   +5 more
openaire   +2 more sources

A recombinant single chain antibody interleukin-2 fusion protein [PDF]

British Journal of Cancer, 1993
Recombinant interleukin-2 (rIL-2) therapy has been shown to be of value in the treatment of some cases of melanoma and renal cell carcinoma. However, its use can be limited by severe systemic toxicity. Targeting rIL-2 to the tumor should improve the antitumor immune response and decrease the systemic toxicity.
Philip Savage, A. K. So, Robert A. Spooner, Agamemnon A. Epenetos   +3 more
openaire   +5 more sources

TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium

Memorias do Instituto Oswaldo Cruz, 2011
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium flagellin (FliC) protein as an ...
Ariane Guglielmi Ariza Camacho, Laís Helena Teixeira, Daniel Youssef Bargieri, Silvia Beatriz Boscardin, Irene da Silva Soares, Ruth Sonntag Nussenzweig, Victor Nussenzweig, Mauricio Martins Rodrigues   +7 more
doaj   +1 more source

The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27 kD γ-zein sequence

Frontiers in Bioengineering and Biotechnology, 2014
Naturally-occurring storage proteins such as zeins are used as fusion partners for recombinant proteins because they induce the formation of ectopic storage organelles known as protein bodies (PBs) where the proteins are stabilized by intermolecular ...
Anna eHofbauer, Jenny ePeters, Elsa eArcalis, Thomas eRademacher, Johannes eLampel, Francois eEudes, Alessandro eVitale, Eva eStoger   +7 more
doaj   +1 more source

Specific occlusion of murine and human tumor vasculature by VCAM-1-targeted recombinant fusion proteins.

Journal of the National Cancer Institute, 2005
BACKGROUND The tumor vasculature is increasingly recognized as a target for cancer therapy. We developed and evaluated recombinant fusion proteins targeting the coagulation-inducing protein soluble tissue factor (sTF) to the luminal tumor endothelial ...
Ariane Dienst, A. Grunow, M. Unruh, B. Rabausch, J. Nör, J. Fries, C. Gottstein   +6 more
semanticscholar   +1 more source

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

Viruses, 2018
The baculovirus expression system (BES) is considered to be a very powerful tool for the expression of numerous difficult to express vertebrate proteins.
Won Seok Gwak, Jae Bang Choi, Beom Ku Han, Sung Min Bae, Soo Dong Woo   +4 more
doaj   +1 more source

Plasmid Display for Stabilization of Enzymes Inside the Cell to Improve Whole-Cell Biotransformation Efficiency

Frontiers in Bioengineering and Biotechnology, 2020
Recombinant whole-cell biocatalysts are widely used for biotransformation of valuable products. However, some key enzymes involved in biotransformation processes are unstable and cannot be easily expressed in the functional form.
Yunjeong Park, Jonghyeok Shin, Jinkyeong Yang, Hooyeon Kim, Younghun Jung, Hyunseok Oh, Yongjoon Kim, Jaehyeon Hwang, Myeongseo Park, Choongjin Ban, Choongjin Ban, Ki Jun Jeong, Sun-Ki Kim, Dae-Hyuk Kweon, Dae-Hyuk Kweon, Dae-Hyuk Kweon   +15 more
doaj   +1 more source