Results 1 to 10 of about 515,934 (157)

Isolation and characterization of Brochothrix phage ADU4

Virus Research, 2022
B. thermosphacta is a psychrotrophic bacterium that often forms the predominant part of the spoilage microflora of aerobically and anaerobically stored meats.
Abdulkerim Karaynir, Hanife Salih, Bülent Bozdoğan, Özgür Güçlü, Dilek Keskin   +4 more
doaj   +1 more source

Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers

Frontiers in Molecular Biosciences, 2022
Antigen-binding variable domains of the H chain of heavy-chain antibodies (VHHs), also known as nanobodies (Nbs), are of great interest in imaging technique, disease prevention, diagnosis, and therapy.
Shuangying Chao, Shuangying Chao, Yuhang Liu, Yuhang Liu, Ning Ding, Ning Ding, Yue Lin, Yue Lin, Qian Wang, Qian Wang, Junwen Tan, Junwen Tan, Wei Li, Wei Li, Yang Zheng, Yang Zheng, Xuejun Hu, Xuejun Hu, Junming Li   +18 more
doaj   +1 more source

Triple-negative breast cancer: understanding Wnt signaling in drug resistance

Cancer Cell International, 2021
Triple-negative breast cancer (TNBC) is not as prevalent as hormone receptor or HER2-positive breast cancers and all receptor tests come back negative.
Parnaz Merikhian, Mohammad Reza Eisavand, Leila Farahmand   +2 more
doaj   +1 more source

Stabilizing and Anti-Repressor Elements Effectively Increases Transgene Expression in Transfected CHO Cells

Frontiers in Bioengineering and Biotechnology, 2022
Chinese hamster ovary (CHO) cells are currently the most widely used host cells for recombinant therapeutic protein (RTP) production. Currently, the RTP yields need to increase further to meet the market needs and reduce costs.
Qin Li, Qin Li, Rui-Fang Yan, Yong-Xiao Yang, Chun-liu Mi, Chun-liu Mi, Yan-long Jia, Tian-Yun Wang, Tian-Yun Wang   +8 more
doaj   +1 more source

Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells

Frontiers in Bioengineering and Biotechnology, 2022
In recent years, mammalian cells have become the primary host cells for the production of recombinant therapeutic proteins (RTPs). Despite that the expression of RTPs in mammalian cells can be improved by directly optimizing or engineering the expression
Mian Wei, Mian Wei, Chun-Liu Mi, Chang-Qin Jing, Tian-Yun Wang   +4 more
doaj   +1 more source

Dual blockade of CD47 and CD24 signaling using a novel bispecific antibody fusion protein enhances macrophage immunotherapy

Molecular Therapy: Oncolytics, 2023
CD47 and its receptor signal regulatory protein α (SIRPα) act as a dominant antiphagocytic, “don’t eat me” signal. Recent studies reveal CD24 as a novel target for cancer immunotherapy by macrophages in ovarian cancer and breast cancer.
Yun Yang, He Wu, Yan Yang, Yan Kang, Runjia He, Bei Zhou, Huaizu Guo, Jing Zhang, Jianqin Li, Chunpo Ge, Tianyun Wang   +10 more
doaj   +1 more source

Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese Hamster Ovary Cells

Frontiers in Bioengineering and Biotechnology, 2022
Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals. Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of most RTPs approved for marketing because of their high-density suspension growth ...
Zheng-Mei Li, Zheng-Mei Li, Zhen-Lin Fan, Zhen-Lin Fan, Xiao-Yin Wang, Xiao-Yin Wang, Tian-Yun Wang, Tian-Yun Wang   +7 more
doaj   +1 more source

Immunotherapeutic targeting of membrane Hsp70-expressing tumors using recombinant human granzyme B [PDF]

, 2012
Background: We have previously reported that human recombinant granzyme B (grB) mediates apoptosis in membrane heat shock protein 70 (Hsp70)-positive tumor cells in a perforin-independent ...
AL Rerole, Alan G. Pockley, Andreas Kirschner, Axel Walch, Brigitte T. Doß, C Botzler, C Botzler, C Gross, D Kaiserman, D Schilling, DR Ciocca, E Schmitt, FC Kurschus, G MacDonald, G Multhoff, G Multhoff, Gabriele Multhoff, Gemma A. Foulds, H Dertinger, J Friedrich, J Friedrich, J Nylandsted, J Sun, J Thiery, JA Trapani, K Pfister, L Chavez-Galan, M Gehrmann, M Gehrmann, M Gehrmann, M Gehrmann, M Gehrmann, M Wang, Mathias Gehrmann, Michael Platten, MS Buzza, RZ Lin, S Balkow, S Mahrus, S Stangl, S Stangl, SP Cullen, Stefan Stangl, SW Krause, U Giesubel, V Milani, WA Boivin, Wolfgang Sievert, Y Liu, Y Liu, Z Xia   +50 more
core   +17 more sources

Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells

Frontiers in Bioengineering and Biotechnology, 2021
As the most widely used mammalian cell line, Chinese hamster ovary (CHO) cells can express various recombinant proteins with a post translational modification pattern similar to that of the proteins from human cells.
Huan-Yu Zhang, Huan-Yu Zhang, Zhen-Lin Fan, Zhen-Lin Fan, Tian-Yun Wang, Tian-Yun Wang   +5 more
doaj   +1 more source

Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells

Frontiers in Bioengineering and Biotechnology, 2021
At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs.
Weifeng Li, Zhenlin Fan, Yan Lin, Tian-Yun Wang, Tian-Yun Wang   +4 more
doaj   +1 more source