Results 21 to 30 of about 1,327,803 (301)

An outlook on coronavirus disease 2019 detection methods

Journal of Pharmaceutical Analysis, 2022
Diagnostic testing plays a fundamental role in the mitigation and containment of coronavirus disease 2019 (COVID-19), as it enables immediate quarantine of those who are infected and contagious and is essential for the epidemiological characterization of
Zahra Hassani Nejad, Fataneh Fatemi, Seyed Ehsan Ranaei Siadat   +2 more
doaj   +1 more source

Production of a soluble and functional recombinant apolipoproteinD in the Pichia pastoris expression system [PDF]

, 2016
ApolipoproteinD (ApoD) is a human glycoprotein from the lipocalin family. ApoD contains a conserved central motif of an 8-stranded antiparallel β-sheet, which forms a beta-barrel that can be used for transport and storage of diverse hydrophobic ligands ...
Armanmehr, S., Kalhor, H.R., Tabarraei, A.   +2 more
core   +1 more source

Engineering a novel endopeptidase based on SARS 3CLpro

BioTechniques, 2009
A 3C-like protease (3CLpro) from the severe acute respiratory syndrome–coronavirus (SARS-CoV) is required for viral replication, cleaving the replicase polyproteins at 11 sites with the conserved Gln↓(Ser, Ala, Gly) sequences. In this study, we developed
Chih-Jung Kuo, Yan-Ping Shih, Daphne Kan, Po-Huang Liang   +3 more
doaj   +1 more source

Russell-like bodies in plant seeds share common features with prolamin bodies and occur upon recombinant protein production [PDF]

, 2019
Although many recombinant proteins have been produced in seeds at high yields without adverse effects on the plant, endoplasmic reticulum (ER) stress and aberrant localization of endogenous or recombinant proteins have also been reported.
Arcalis, Elsa, Avesani, Linda, Bortesi, Luisa, De Meyer, Thomas, de meyer, thomas, Depicker, Anna, Hilscher, Julia, Ibl, Verena, Morandini, Francesca, Pezzotti, Mario, Pum, Dietmar, Rademacher, Thomas, Stoger, Eva, Vitale, Alessandro   +13 more
core   +2 more sources

Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae

Bioactive Materials, 2023
As a renewable and sustainable source for energy, environment, and biomedical applications, microalgae and microalgal biodiesel have attracted great attention.
Guoxia Zheng, Yutong Cui, Ling Lu, Ming Guo, Xuejun Hu, Lin Wang, Shuping Yu, Shenxia Sun, Yuancheng Li, Xingcai Zhang, Yunhua Wang   +10 more
doaj   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman, Atkinson, Baekkeskov, Baekkeskov, Berthold, Boitard, Bottazzo, Castano, Dean, Frorath, Goedel, Greenbaum, Harrison, Heike Berg, Hopp, Jochen Seissler, Karjalainen, Kaufman, Kuglin, Kuglin, Ludwig Mauch, Michael Walter, Palmer, Patrick, Seissler, Smith, Smith, Snyder, Su, Wolfgang Northemann   +29 more
core   +1 more source

Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification

Frontiers in Microbiology, 2018
Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for ...
Claudia Ortega, Claudia Ortega, Daniel Prieto, Daniel Prieto, Cecilia Abreu, Cecilia Abreu, Pablo Oppezzo, Pablo Oppezzo, Agustín Correa, Agustín Correa   +9 more
doaj   +1 more source

19F NMR spectroscopy monitors ligand binding to recombinantly fluorine-labelled b'x from human protein disulphide isomerase (hPDI) [PDF]

, 2014
We report a protein-observe (19)F NMR-based ligand titration binding study of human PDI b'x with ?-somatostatin that also emphasises the need to optimise recombinant protein fluorination when using 5- or 6-fluoroindole.
Byrne, Crowley, Darby, Denisa Doko, Denisov, Fielding, Freedman, Hatahet, Hatahet, Kirsty L. Richards, Klappa, Koivunen, Mark J. Howard, Michelle L. Rowe, Nakasako, Nguyen, Pirneskoski, Richard A. Williamson, Rose Curtis-Marof, Serve, Tian, Wallis, Wang, Wang, Williamson   +24 more
core   +1 more source

Noncontiguous SCHEMA Protein Recombination [PDF]

, 2014
SCHEMA is a method of designing protein recombination libraries that contain a large fraction of functional proteins with a high degree of mutational diversity. In the previous chapter, we illustrated the method for designing libraries by swapping contiguous sequence elements.
Smith, Matthew A., Arnold, Frances H.
openaire   +3 more sources

Population, sexual and reproductive health, rights and sustainable development: forging a common agenda. [PDF]

, 2010
This article suggests that sexual and reproductive health and rights activists seeking to influence the post-2015 international development paradigm must work with sustainable development advocates concerned with a range of issues, including climate ...
Campbell, D. M., Carter, P. E., Collins-Emerson, J. M., French, N. P., Hathaway, S., Marshall, J., Midwinter, A. C., Mullner, P., Pirie, R., Shadbolt, T., Spencer, Simon E. F., Wilson, D. J.   +11 more
core   +1 more source