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Recombinant protein expression in Sulfolobus islandicus
2021Since its invention, recombinant protein expression has greatly facilitated our understanding of various cellular processes in different biological systems because theoretically this technique renders any gene to be expressed in a mesophilic host like Escherichia coli, thus allowing functional characterizations of proteins of interest.
Xu, Feng, Qunxin, She
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Recombinant Protein Expression in Leishmania tarentolae
Molecular Biotechnology, 2009A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested
Giancarlo, Basile, Manuela, Peticca
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Recombinant Protein Expression in Nicotiana
2010Recombinant protein pharmaceuticals are now widely used in treatment of chronic diseases, and several recombinant protein subunit vaccines are approved for human and veterinary use. With growing demand for complex protein pharmaceuticals, such as monoclonal antibodies, manufacturing capacity is becoming limited.
Nobuyuki, Matoba +2 more
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Biotechnology progress (Print), 2018
The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such as expression of recombinant proteins, requires media that support growth and production.
Frank V Ritacco +2 more
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The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such as expression of recombinant proteins, requires media that support growth and production.
Frank V Ritacco +2 more
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Recombinant Expression and Purification of Smad Proteins
Protein Expression and Purification, 2000Smads transduce intracellular signals initiated by members of the transforming growth factor beta (TGF beta) family, including activins, TGF betas, and bone morphogenetic proteins. Recently, various models concerning the mechanism of Smad action have been proposed; however, these models are basically qualitative.
M, Funaba, L S, Mathews
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The expression of recombinant proteins in yeasts
Current Opinion in Biotechnology, 1996The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically ...
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Expression of Recombinant Proteins in Pichia Pastoris
Applied Biochemistry and Biotechnology, 2007Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression.
Pingzuo, Li +6 more
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Recombinant protein expression
2013This chapter zooms in on technical issues related to the systems commonly used for recombinant protein production and discusses the advantages and disadvantages of each. Among these expression systems are E. coli, Lactobacillus lactis, Saccharomyces cerevisiae, Pichia pastoris, insect larval cells, mammalian cells, and cell-free systems.
Michael Harrison, Michael J. McPherson
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Understanding recombinant expression of membrane proteins
Current Opinion in Biotechnology, 2006Over the past 15 years, numerous reports have been published on the recombinant expression of integral membrane proteins. Some proteins accumulate in the membrane to high levels, whereas other often closely related proteins are barely detected. Understanding the underlying reasons for this variation has proven difficult.
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Protein Heterogeneity in Recombinant Protein Expression
2023Recombinant proteins are commonly utilized in the drug discovery pipeline as a means of assessing the potential drug’s potency. However, when expressed, heterogeneity in the protein’s sequence and/or structure can occur, thereby affecting experimental results.
BRIAN MCINNIS +4 more
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