Results 231 to 240 of about 6,659,682 (289)
Some of the next articles are maybe not open access.
Recombinant protein expression for therapeutic applications
Current Opinion in Biotechnology, 2002In recent years, the number of recombinant proteins used for therapeutic applications has increased dramatically. Many of these applications involve complex glycoproteins and antibodies with relatively high production needs. These demands have driven the development of a variety of improvements in protein expression technology, particularly involving ...
Dana C, Andersen, Lynne, Krummen
exaly +3 more sources
Transient expression of recombinant proteins in plants
2021Transient protein expression in plant cells is less time consuming than the production of whole transgenic plants. For transient expression, agroinfiltration is a simple and effective method to deliver transgenes into plant cells. After an Agrobacterium infection, recombinant proteins can be produced in plant cells from 3 to 10days. To increase protein
Shohei, Nosaki, Kenji, Miura
openaire +2 more sources
Recombinant Protein Expression in Nicotiana
2010Recombinant protein pharmaceuticals are now widely used in treatment of chronic diseases, and several recombinant protein subunit vaccines are approved for human and veterinary use. With growing demand for complex protein pharmaceuticals, such as monoclonal antibodies, manufacturing capacity is becoming limited.
Nobuyuki, Matoba +2 more
openaire +2 more sources
Recombinant Protein Expression in Leishmania tarentolae
Molecular Biotechnology, 2009A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested
Giancarlo, Basile, Manuela, Peticca
openaire +2 more sources
Recombinant Expression and Purification of Smad Proteins
Protein Expression and Purification, 2000Smads transduce intracellular signals initiated by members of the transforming growth factor beta (TGF beta) family, including activins, TGF betas, and bone morphogenetic proteins. Recently, various models concerning the mechanism of Smad action have been proposed; however, these models are basically qualitative.
M, Funaba, L S, Mathews
openaire +2 more sources
Recombinant protein expression in Sulfolobus islandicus
2021Since its invention, recombinant protein expression has greatly facilitated our understanding of various cellular processes in different biological systems because theoretically this technique renders any gene to be expressed in a mesophilic host like Escherichia coli, thus allowing functional characterizations of proteins of interest.
Xu, Feng, Qunxin, She
openaire +2 more sources
The expression of recombinant proteins in yeasts
Current Opinion in Biotechnology, 1996The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically ...
openaire +2 more sources
Effects of Oxygen on Recombinant Protein Expression
Biotechnology Progress, 1998Efforts to increase cell growth and protein yields need to be complemented by the maintenance of the quality of the protein produced. Elevated oxygen pressure or rapid increases in oxygen content can cause oxidative stress within the cells, leading to oxidation of specific proteins and nucleotide sequences. In addition, transient or steady-state anoxic
J O, Konz, J, King, C L, Cooney
openaire +2 more sources
Recombinant protein expression
2013This chapter zooms in on technical issues related to the systems commonly used for recombinant protein production and discusses the advantages and disadvantages of each. Among these expression systems are E. coli, Lactobacillus lactis, Saccharomyces cerevisiae, Pichia pastoris, insect larval cells, mammalian cells, and cell-free systems.
Michael Harrison, Michael J. McPherson
openaire +1 more source
Recombinant cloning strategies for protein expression
Current Opinion in Structural Biology, 2016A variety of methods to create specific constructs for protein expression, in a broad range of organisms, are available nowadays. Restriction enzyme-free, ligation-independent and recombinase-based cloning methods have enabled high-throughput protein expression for structural and functional studies.
Patrick Hn, Celie +2 more
openaire +2 more sources