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Protein Heterogeneity in Recombinant Protein Expression
2023Recombinant proteins are commonly utilized in the drug discovery pipeline as a means of assessing the potential drug’s potency. However, when expressed, heterogeneity in the protein’s sequence and/or structure can occur, thereby affecting experimental results.
BRIAN MCINNIS +4 more
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Transient expression of recombinant proteins in plants
2021Transient protein expression in plant cells is less time consuming than the production of whole transgenic plants. For transient expression, agroinfiltration is a simple and effective method to deliver transgenes into plant cells. After an Agrobacterium infection, recombinant proteins can be produced in plant cells from 3 to 10days. To increase protein
Shohei, Nosaki, Kenji, Miura
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Understanding recombinant expression of membrane proteins
Current Opinion in Biotechnology, 2006Over the past 15 years, numerous reports have been published on the recombinant expression of integral membrane proteins. Some proteins accumulate in the membrane to high levels, whereas other often closely related proteins are barely detected. Understanding the underlying reasons for this variation has proven difficult.
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Recombinant protein expression in Escherichia coli
Current Opinion in Biotechnology, 1999Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E.
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Effects of Oxygen on Recombinant Protein Expression
Biotechnology Progress, 1998Efforts to increase cell growth and protein yields need to be complemented by the maintenance of the quality of the protein produced. Elevated oxygen pressure or rapid increases in oxygen content can cause oxidative stress within the cells, leading to oxidation of specific proteins and nucleotide sequences. In addition, transient or steady-state anoxic
J O, Konz, J, King, C L, Cooney
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Recombinant Expression and Purification of Smad Proteins
Protein Expression and Purification, 2000Smads transduce intracellular signals initiated by members of the transforming growth factor beta (TGF beta) family, including activins, TGF betas, and bone morphogenetic proteins. Recently, various models concerning the mechanism of Smad action have been proposed; however, these models are basically qualitative.
M, Funaba, L S, Mathews
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Expression of Recombinant Proteins in Pichia Pastoris
Applied Biochemistry and Biotechnology, 2007Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression.
Pingzuo, Li +6 more
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Evaluating Heterogeneity of Recombinant Protein Expression
2023Journal of Student-Scientists' Research, Vol. 5 (2023)
LUKE MANTOOTH +4 more
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The Cerebellum, 2008
Friedreich's ataxia is an autosomal recessive neurodegenerative disease that is due to the loss of function of the frataxin protein. The molecular basis of this disease is still a matter of debate and treatments have so far focused on managing symptoms. Drugs that can increase the amount of frataxin protein offer a possible therapy for the disease. One
ACQUAVIVA, Fabio +8 more
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Friedreich's ataxia is an autosomal recessive neurodegenerative disease that is due to the loss of function of the frataxin protein. The molecular basis of this disease is still a matter of debate and treatments have so far focused on managing symptoms. Drugs that can increase the amount of frataxin protein offer a possible therapy for the disease. One
ACQUAVIVA, Fabio +8 more
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ADAMTS7: Recombinant Protein Expression and Purification
2019ADAMTS7 is a secreted protease that is predominantly expressed in tissues of the cardiovascular system and tendon. Although recent evidence suggests that it may play a role in the etiology of coronary artery disease, its physiological function and substrates are unknown.
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