Results 241 to 250 of about 1,066,221 (297)

Conformable Microelectrode Arrays Integrated with a Scoop‐Shaped Slide‐Well for Dynamic Electrophysiological Profiling of Patient‐Derived Cardiac Organoids

Advanced Science, EarlyView.
A flexible, ultrathin multi‐channel microelectrode array (MEA) conformally integrated onto a curved slide‐well enables stable electrical interfacing with 3D cardiac organoids. The geometry‐guided self‐alignment allows simultaneous recording of extracellular field potentials and contractile motion from irregular, beating tissues.
Ye Seul Kim, Jeonghwa Jeong, Gyeonghwa Heo, Young Woo Kwon, Jeongha Lee, Yoon Ji Jeong, Gyu Tae Park, Seongdo Jeong, Ki Won Hwang, Murim Choi, Jinhong Shin, Suck Won Hong, Jae Ho Kim   +12 more
wiley   +1 more source

NPM1 Mediates mRNA Sorting into Extracellular Vesicles via Specific RNA Motif Binding and Phase Separation

Advanced Science, EarlyView.
NPM1 selectively sorts specific mRNAs into extracellular vesicles (EVs) by recognizing RNA motifs and forming phase‐separated condensates. These mRNA cargos—including EGFR—are then loaded via multivesicular bodies. This active sorting pathway, validated in EVs derived from both lung cancer cells and patient serum, reveals a specific mechanism for ...
Kaixiang Zhang, Gaoge Sun, Xiangzheng Liu, Shuai Zuo, Pengyuan Wang, Boxiao Zhan, Zhi John Lu, Hang Yin, Ying Zhang   +8 more
wiley   +1 more source

Improvement of solubility and yield of recombinant protein expression in E. coli using a two-step system.

Res Pharm Sci, 2019
Sadeghian-Rizi T, Ebrahimi A, Moazzen F, Yousefian H, Jahanian-Najafabadi A.   +4 more
europepmc   +1 more source

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Recombinant protein expression for therapeutic applications

Current Opinion in Biotechnology, 2002
In recent years, the number of recombinant proteins used for therapeutic applications has increased dramatically. Many of these applications involve complex glycoproteins and antibodies with relatively high production needs. These demands have driven the development of a variety of improvements in protein expression technology, particularly involving ...
Dana C, Andersen, Lynne, Krummen
openaire   +4 more sources

Recombinant protein expression in Sulfolobus islandicus

2021
Since its invention, recombinant protein expression has greatly facilitated our understanding of various cellular processes in different biological systems because theoretically this technique renders any gene to be expressed in a mesophilic host like Escherichia coli, thus allowing functional characterizations of proteins of interest.
Xu, Feng, Qunxin, She
openaire   +2 more sources

Recombinant Protein Expression in Leishmania tarentolae

Molecular Biotechnology, 2009
A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested
Giancarlo, Basile, Manuela, Peticca
openaire   +2 more sources

Recombinant Protein Expression in Nicotiana

2010
Recombinant protein pharmaceuticals are now widely used in treatment of chronic diseases, and several recombinant protein subunit vaccines are approved for human and veterinary use. With growing demand for complex protein pharmaceuticals, such as monoclonal antibodies, manufacturing capacity is becoming limited.
Nobuyuki, Matoba, Keith R, Davis, Kenneth E, Palmer   +2 more
openaire   +2 more sources

Recombinant protein expression in Pichia pastoris

Molecular Biotechnology, 2000
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool.
J M, Cregg, J L, Cereghino, J, Shi, D R, Higgins   +3 more
openaire   +2 more sources

Recombinant protein expression

2013
This chapter zooms in on technical issues related to the systems commonly used for recombinant protein production and discusses the advantages and disadvantages of each. Among these expression systems are E. coli, Lactobacillus lactis, Saccharomyces cerevisiae, Pichia pastoris, insect larval cells, mammalian cells, and cell-free systems.
Michael Harrison, Michael J. McPherson
openaire   +1 more source

Recombinant cloning strategies for protein expression

Current Opinion in Structural Biology, 2016
A variety of methods to create specific constructs for protein expression, in a broad range of organisms, are available nowadays. Restriction enzyme-free, ligation-independent and recombinase-based cloning methods have enabled high-throughput protein expression for structural and functional studies.
Patrick Hn, Celie, Annabel Ha, Parret, Anastassis, Perrakis   +2 more
openaire   +2 more sources