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Expression of Recombinant Proteins in Pichia Pastoris
Applied Biochemistry and Biotechnology, 2007Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression.
Pingzuo, Li +6 more
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Recombinant protein expression in Pichia pastoris
Molecular Biotechnology, 2000The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool.
J M, Cregg +3 more
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Understanding recombinant expression of membrane proteins
Current Opinion in Biotechnology, 2006Over the past 15 years, numerous reports have been published on the recombinant expression of integral membrane proteins. Some proteins accumulate in the membrane to high levels, whereas other often closely related proteins are barely detected. Understanding the underlying reasons for this variation has proven difficult.
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Recombinant protein expression in Escherichia coli
Current Opinion in Biotechnology, 1999Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E.
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ADAMTS7: Recombinant Protein Expression and Purification
2019ADAMTS7 is a secreted protease that is predominantly expressed in tissues of the cardiovascular system and tendon. Although recent evidence suggests that it may play a role in the etiology of coronary artery disease, its physiological function and substrates are unknown.
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Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21
Frontiers in Microbiology, 2021Julia Gallego-Jara +2 more
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Bacterial expression systems for recombinant protein production: E. coli and beyond
Biotechnology Advances, 2012Rachel Chen
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Recombinant protein expression in Escherichia coli: advances and challenges
Frontiers in Microbiology, 2014Eduardo A Ceccarelli, Rosano German L
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