Results 71 to 80 of about 797,107 (316)

Optimisation of the production of cathepsin L1 from a recombinant saccharomyces cerevisiae [PDF]

, 2002
Cathepsin L1 is a cysteine protease that has been previously isolated and functionally expressed in Saccharomyces cerevisiae. It has the potential to be employed as a vaccine for liver-fluke disease in cattle and other ruminants.
O'Donovan, Eimear C
core  

Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology

Journal of Biological Engineering
The sequence of a carboxy-terminal of the β-lactam sensor-transducer protein (BlaR-CTD) from Bacillus licheniformis ATCC14580 was extracted from US7745193B2 patent and expressed in E. coli using pColdI vector as a soluble His-tag recombinant protein.
Mohadeseh Haji Abdolvahab, Mojdeh Safari, Farkhonde Hasannejad, Nika Asefi, Alireza Salimi, Mahboobeh Nazari   +5 more
doaj   +1 more source

Immunodiagnostic Value of Echinococcus Granulosus Recombinant B8/1 Subunit of Antigen B [PDF]

Iranian Journal of Immunology, 2017
Background: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory.
Amir Savardashtaki, Bahador Sarkari, Farzane Arianfar, Zohreh Mostafavi-Pour   +3 more
doaj  

Overview of the Purification of Recombinant Proteins [PDF]

Current Protocols in Protein Science, 2015
AbstractWhen the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughoutCurrent Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the
openaire   +2 more sources

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen, Mauch, Ludwig, Walter, Michael, Northemann, Wolfgang, Berg, Heike   +4 more
core   +1 more source

New insights into Chitosan nano-carriers for RNA delivery and carbon dot imaging for cancer theranostics: challenges and future prospects

Cancer Nanotechnology
Cancer therapy and imaging remain multifaceted global health challenges for patients, investigators, and medical practitioners. RNA-based therapies, facilitated by nanotechnology, show great potential for targeted treatment due to their high specificity ...
Mohammad Jafar Mantashlou, Javad Sarvmeili, Nasrin Mohajeri, Fatemeh Hampaei, Sevil Vaghefi Moghaddam, Effat Alizadeh   +5 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

GRP-based vaccines as a novel approach in cancer immunotherapy: mechanisms, challenges, and prospects

Cancer Cell International
Glucose-regulated proteins (GRPs), key members of the heat shock protein (HSP) family, function as molecular chaperones that are upregulated under endoplasmic reticulum (ER) stress. Prominent GRPs such as GRP78, GRP94/gp96, GRP75, and GRP170 play a great
Razieh Malekian Shahreza, Hamed Zare, Nima Rastegar-Pouyani, Hamid Aria, Fatemeh Hakim, Mohammad Arefnia, Hamid Bakherad   +6 more
doaj   +1 more source

Noncontiguous SCHEMA Protein Recombination [PDF]

, 2014
SCHEMA is a method of designing protein recombination libraries that contain a large fraction of functional proteins with a high degree of mutational diversity. In the previous chapter, we illustrated the method for designing libraries by swapping contiguous sequence elements.
Smith, Matthew A., Arnold, Frances H.
openaire   +3 more sources