Advanced Science, EarlyView.A novel therapy using engineered immune cells (NAC‐T cells) showed promise for refractory malignant mesothelioma. Based on the encouraging preclinical data, the first‐in‐human trial is initiated, demonstrating tolerable safety and promising anti‐tumor activity (ORR 63.6%, DCR 100%, including one CR).
Yan Sun +23 more
wiley +1 more source
Red recombination enables a wide variety of markerless manipulation of porcine epidemic diarrhea virus genome to generate recombinant virus. [PDF]
Front Cell Infect Microbiol, 2023 Pan S, Mou C, Chen Z.
europepmc +1 more source
Attenuation of Influenza a Virus into Live Vaccines Through C‐End Degrons
Advanced Science, EarlyView.Naturally occurring C‐end degrons are exploited to design proteolysis‐targeting live attenuated influenza vaccines. C‐end degron tagging of viral M1 protein promotes proteasome‐dependent degradation, resulting in robust attenuation in host cells while permitting scalable production in engineered cells.
Ping Wang +14 more
wiley +1 more source
A Novel Function of Nonadecanoic Acid in Regulating Glucose Homeostasis
Advanced Science, EarlyView.This study identifies the odd‐chain fatty acid C19:0 as an endogenous ligand of GPR120 that promotes glucose homeostasis via Gαq signaling. In obesity, elevated palmitic acid suppresses endogenous C19:0 production through a miRNA–PPARα–HACL1 pathway, offering a promising direction for understanding the role of OCFAs in human health.
Yanting Hou +14 more
wiley +1 more source
Current status of recombinant duck enteritis virus vector vaccine research
Frontiers in Veterinary ScienceDuck enteritis virus (DEV), the pathogen of duck viral enteritis, belongs to the α-herpesvirus subfamily. Like other herpesviruses, it has a large genome with multiple non-coding and non-essential regions for viral replication.
Wen-Feng Jia +5 more
doaj +1 more source
Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus. [PDF]
iScience, 2023 Chen DY +18 more
europepmc +1 more source
Production of recombinant adeno-associated virus
, 2000 Currently, rAAV appears to be one of the most promising vectors for gene therapy applications. Attractive features of the vector include nonpathogenicity, the ability to infect nondividing cells, escape from host immune responses, and integration into the host genome.
G P, Gao, J M, Wilson, N A, Wivel
openaire +2 more sources
Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications
Advanced Science, EarlyView.Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo +12 more
wiley +1 more source
Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs
Veterinary SciencesThe vesicular stomatitis virus (VSV)-vectored African swine fever virus (ASFV) vaccine can induce efficient immune response, but the potential mechanism remains unsolved.
Yunyun Ma +6 more
doaj +1 more source
Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer Quantification. [PDF]
Viruses, 2023 Korotkaja K, Zajakina A.
europepmc +1 more source