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Single-cell characterization of bacterial optogenetic Cre recombinases [PDF]
iScienceSummary: Microbial optogenetic tools can regulate gene expression with spatial and temporal precision, offering excellent potential for single-cell resolution studies.
Hellen Huang +2 more
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Characterization of recombinase activity across cellular growth phases [PDF]
Scientific ReportsRecombinases are essential enzymes in synthetic biology and biomedical research, enabling site-specific DNA modifications for applications such as gene therapy, the generation of transgenic models, and the construction of genetic circuits.
M. Gonzalez-Colell, J. Macia
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Toxins, 2021 Currently, studies worldwide have comprehensively recognized the importance of Sphingomonadaceae bacteria and the mlrCABD gene cluster in microcystin (MC) degradation. However, knowledge about their degradation of nodularin (NOD) is still unclear.
Mengxuan Yuan +5 more
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Frontiers in Microbiology, 2021 Multiplex engineering at the scale of whole genomes has become increasingly important for synthetic biology and biotechnology applications. Although several methods have been reported for engineering microbe genomes, their use is limited by their complex
Aihua Deng +12 more
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High Resolution View on the Regulation of Recombinase Accumulation in Mammalian Meiosis
Frontiers in Cell and Developmental Biology, 2021 A distinguishing feature of meiotic DNA double-strand breaks (DSBs), compared to DSBs in somatic cells, is the fact that they are induced in a programmed and specifically orchestrated manner, which includes chromatin remodeling prior to DSB induction. In
Aditya N. Mhaskar +6 more
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Archaeal tyrosine recombinases
FEMS Microbiology Reviews, 2021 ABSTRACTThe integration of mobile genetic elements into their host chromosome influences the immediate fate of cellular organisms and gradually shapes their evolution. Site-specific recombinases catalyzing this integration have been extensively characterized both in bacteria and eukarya.
Badel, Catherine +2 more
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Elucidating Recombination Mediator Function Using Biophysical Tools
Biology, 2021 The recombination mediator proteins (RMPs) are ubiquitous and play a crucial role in genome stability. RMPs facilitate the loading of recombinases like RecA onto single-stranded (ss) DNA coated by single-strand binding proteins like SSB.
Camille Henry, Sarah S. Henrikus
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SOS-Independent Pyocin Production in P. aeruginosa Is Induced by XerC Recombinase Deficiency
mBio, 2021 Pyocins are phage tail-like protein complexes that can be used by Pseudomonas aeruginosa to enact intraspecies competition by killing competing strains. The pyocin gene cluster also encodes holin and lysin enzymes that lyse producer cells to release the ...
Nina S. Baggett +2 more
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Inhibition of p53 and ATRX increases telomeric recombination in primary fibroblasts
FEBS Open Bio, 2023 Telomere length can be maintained either by the telomerase enzyme or by alternative lengthening of telomeres (ALT), which is based on telomeric recombination. However, both mechanisms are inactive in most human somatic cells.
Ion Udroiu +2 more
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Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes
Frontiers in Plant Science, 2022 Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added to existing transgenic lines, site-specific integration
Qian Yin, Qian Yin, Ruyu Li, David W. Ow
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