Results 111 to 120 of about 99,506 (245)

DNA Flap‐Mediated Control of Transcription for Programmable RNA Synthesis

Angewandte Chemie International Edition, EarlyView.
Pyrimidine‐rich ssDNA flaps on the T7 promoter inhibit T7 RNA polymerase (T7RP) transcription. Leveraging this pyrimidine bias, we developed DNAzyme‐ and MNAzyme‐mediated flap promoter induced transcription control (D‐FIT and M‐FIT) systems. These platforms enable regulated transcription and sequence‐specific detection, highlighting the programmable ...
Eun Sung Lee, Jisu Woo, Seokjoon Kim, Seok Hyeon Kim, Gun Haeng Lee, Ki Soo Park   +5 more
wiley   +1 more source

Histological Characterization and Comparative Transcriptomic Analysis of Gonads During Early Sex Differentiation in the Northern Snakehead (Channa argus)

Animal Research and One Health, EarlyView.
This study delineates the early sex differentiation timeline in the northern snakehead (Channa argus). These findings provide a foundation for future sex‐control breeding in this economically important species. ABSTRACT The northern snakehead (Channa argus) is an economically important aquaculture species in China.
Chaonan Sun, Donglei Sun, Haishen Wen, Xin Qi, Chao Li, Xiaoyan Zhang, Yun Li   +6 more
wiley   +1 more source

Altered Brain Structure in an ATRX‐Deficient Mouse Model of Autism Spectrum Disorder

Autism Research, EarlyView.
ABSTRACT Mutations in the ATRX gene are a primary cause of alpha‐thalassemia intellectual disability X‐linked (ATRX) syndrome, which is characterized by intellectual disability, autism, and a range of brain structural abnormalities, including microcephaly.
Katherine Quesnel, Jacob Ellegood, Jason P. Lerch, Nathalie G. Bérubé   +3 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus

Антибиотики и Химиотерапия, 2020
Coagulase-negative staphylococci (CNS), in particular Staphylococcus haemolyticus, play an important role in the etiology of nosocomial infections. Most CNS are resistant to beta-lactam antibiotics, which is realized through the production of the second ...
A. V. Polonskaya, M. A. Kornienko, A. I. Manolov, N. S. Kuptsov, G. B. Smirnov, L. A. Lyubasovskaya, T. V. Priputnevich, E. A. Shitikov, E. N. Ilina   +8 more
doaj  

Whole tissue imaging of cellular boundaries at sub‐micron resolutions for deep learning cell segmentation: Applications in the analysis of epithelial bending of ectoderm

Developmental Dynamics, EarlyView.
Abstract Background To understand cellular morphology, biologists have relied on traditional optical microscopy of tissues combined with tissue clearing protocols to image structures deep within tissues. Unfortunately, these protocols often struggle to retain cell boundary markers, especially at high enough resolutions necessary for precise cell ...
Sam C. P. Norris, Jimmy K. Hu, Neil H. Shubin   +2 more
wiley   +1 more source

Heterozygous Med13l mice recapitulate a developmental growth delay and craniofacial anomalies seen in MED13L syndrome

Developmental Dynamics, EarlyView.
Abstract Background Gene transcription is crucial for embryo and postnatal development and is regulated by the Mediator complex. Mediator is comprised of four submodules, including the kinase submodule (CKM). The CKM consists of MED13, MED12, CDK8, and CCNC.
Anna K. Leinheiser, Timothy T. Nguyen, Kayla M. Henry, Mariela Rosales, Eric Van Otterloo, Chad E. Grueter   +5 more
wiley   +1 more source

Expression of mutant TIE2 p.L914F during mouse development causes embryonic lethality and defects in vascular remodeling

Developmental Dynamics, EarlyView.
Abstract Background Sporadic venous malformation (VM) is associated with the hyperactivating p.L914F mutation in TIE2, a receptor tyrosine kinase essential for vascular development. This mutation is not found in hereditary VM, suggesting incompatibility with life when expressed during early vascular development.
Lindsay J. Bischoff, Sandra Schrenk, Kara Soroko, Chhiring Sherpa, Ashok Arasu, Damien Reynaud, Elisa Boscolo   +6 more
wiley   +1 more source

Long‐lasting remodeling of astrocytes in an Scna1+/− mouse model of Dravet syndrome

Epilepsia, EarlyView.
Abstract Objective Dravet syndrome (DS) is a prototypical developmental and epileptic encephalopathy caused by mutations in the SCN1A gene, leading to loss of function of the voltage‐gated sodium channel Naᵥ1.1. The latter causes early onset drug‐resistant seizures and enduring cognitive and behavioral deficits.
Athénaïs Genin, Alicia Janvier, Tristan Moujellil‐Legagneur, Marine Blaquière, Alexis Chaussy, Romane Privé, Fabrice Duprat, Massimo Mantegazza, Etienne Audinat, Nicola Marchi, Noémie Cresto   +10 more
wiley   +1 more source

Spherical Nucleic Acid Stabilized Cage Type Three‐Dimensional Electrochemiluminescence Probe for Sensitive and Amplification‐Free Diagnosis of Infectious Diseases

Exploration, EarlyView.
This work developed a spherical nucleic acid stabilized cage‐type 3D electrochemiluminescence reporter probe for amplification‐free and ultrasensitive detection of pathogenic. Furthermore, a hand‐held detection device was designed, and we established a straightforward workflow enabling sample to answer within 15 min.
Yu Fu, Wenlu Song, Jinyan Lin, Wei Wang, Lunjing Liu, Wenjie Wu, Huiyi Yang, Jingru Wang, Yang Yang, Bowen Shu, Yideng Jiang, Yuhui Liao   +11 more
wiley   +1 more source