Results 131 to 140 of about 99,506 (245)

CRISPR/Cas9 Genome Engineering in Non‐Conventional Oleaginous Yeasts: Applications, Challenges, and Prospects

Yeast, EarlyView.
ABSTRACT Given the biotechnological potential of yeast‐derived oils for oleochemical production, genes encoding lipid metabolism enzymes are key targets for metabolic engineering. Genetic engineering tools such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, Transcription Activator‐Like Effector Nucleases (TALENs), Zinc ...
Rodrigo Gonçalves Dias, Fernanda Pinheiro Moreira Freitas, Eduardo Luís Menezes de Almeida, Luciano Gomes Fietto, Agustin Zsögön, Wendel Batista da Silveira   +5 more
wiley   +1 more source

Rapid detection of the beech leaf nematode, Litylenchus crenatae using recombinase polymerase amplification assay with lateral flow dipsticks. [PDF]

PLoS One
Kantor M, Subbotin SA.
europepmc   +1 more source

Sensitizer‐Induced Basophils Accelerate Skin Re‐Epithelialization via IL‐4/IL‐13‐Mediated Macrophage Polarization

Allergy, EarlyView.
Oxazolone exposure drives rapid basophil recruitment to wounded skin, accelerating re‐epithelialization, angiogenesis, and tissue regeneration. Basophils are the dominant early source of IL‐4 and IL‐13 in the wound microenvironment. Basophil‐derived IL‐4/IL‐13 promote macrophage polarization toward a pro‐repair M2 phenotype, enabling efficient wound ...
Yufei Zhang, Xueting Peng, Kaixuan Ren, Shiran Kang, Zihan Xue, Yazhuo Li, Zhu Yan, Rongfang Feng, Min Gao, Qin Chen, Xiaoying Ning, Fan Bai, Liesu Meng, Yumin Xia, Yale Liu   +14 more
wiley   +1 more source

Rapid Detection of Fungi in Nail Specimens Using Multi-Tube Recombinase Polymerase Amplification Assay. [PDF]

Mycoses
Wu R, Li M, Du W, Chen Z, Yin S, Hu L, Xi L, Zhang J, Huang H.   +8 more
europepmc   +1 more source

An intracellular recombinant single‐chain variable antibody fragment as a new class of phosphodiesterase type 5 inhibitors

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz, Sophie Sprenger, Olga Schweigert, Sanika Mohagaonkar, Matti Walsdorff, Tanja Zeller, Filip Berisha, Viacheslav O. Nikolaev, Sergei D. Rybalkin   +8 more
wiley   +1 more source

Directed evolution on compact landing pads yields highly efficient recombinases for large DNA integration. [PDF]

Nucleic Acids Res
Kim H, Kang HG, Oh Y, Lee WJ, Lee Y, Kim EK, Nam KH, Lee DS, Lee SH, Sim BW.   +9 more
europepmc   +1 more source

Cannabigerol reverses mechanical allodynia through α2A‐adrenergic modulation of thalamocortical signaling in chemotherapy‐induced neuropathy

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Chemotherapy‐induced peripheral neuropathy (CIPN) is a prevalent and treatment‐resistant side effect of platinum‐based chemotherapy, characterised by mechanical allodynia. Cannabigerol (CBG), a non‐psychoactive cannabinoid, has shown antinociceptive potential, but its site and mechanism of action remain unclear.
Quinn W. Wade, Nathan Morris, Diana E. Sepulveda, Alonso Cortez‐Resendiz, Christopher Brandl, Jennifer E. Lausch, Mariya Starostina, Nicholas M. Graziane   +7 more
wiley   +1 more source

Correction: Yehia et al. Rapid Detection Assay for Infectious Bronchitis Virus Using Real-Time Reverse Transcription Recombinase-Aided Amplification. Viruses 2025, 17, 1172. [PDF]

Viruses
Yehia N, Abd El Wahed A, Arafa A, Said D, Mohamed AAE, Eid S, Shalaby MA, Kobialka RM, Truyen U, Ceruti A.   +9 more
europepmc   +1 more source

Dissociating role of Bassoon in glutamatergic and dopaminergic neurons in alcohol‐related behaviour and affective state in mice

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Alcohol abuse and affective disorders are severe comorbid psychiatric diseases characterized by impaired brain synaptic transmission. The role of presynaptic scaffolding proteins coordinating presynaptic plasticity and neurotransmitter release, such as Bassoon (Bsn), in the pathogenesis of these disorders remains elusive.
Liubov S. Kalinichenko, Mona Krenbauer, Janosh Isert, Marlon Kamlah, Thorben Tanzer, Hannes Rödel, Tobias Müller, Anil Annamneedi, Enes Yağız Akdaş, Volker Eulenburg, Bernd Lenz, Eckart D. Gundelfinger, Johannes Kornhuber, Anna Fejtova, Christian P. Müller   +14 more
wiley   +1 more source

A combinatorial synthetic strategy for developing genome-editing protein-delivery agents targeting mouse retina. [PDF]

Nat Commun
Zhang J, Hołubowicz R, Smidak R, Hu Y, Du SW, Felgner JH, Palczewska G, Rodrigues Menezes C, Risaliti E, Dong Z, Ma X, Shayegan MH, Chen PZ, Xing L, Hołubowicz M, Li B, Liu DR, Felgner PL, Tochtrop GP, Palczewski K.   +19 more
europepmc   +1 more source