Results 11 to 20 of about 99,506 (245)
Programmable RNA-Guided Large DNA Transgenesis by CRISPR/Cas9 and Site-Specific Integrase Bxb1
Frontiers in Bioengineering and Biotechnology, 2022 The inability to insert large DNA constructs into the genome efficiently and precisely is a key challenge in genomic engineering. Random transgenesis, which is widely used, lacks precision, and comes with a slew of drawbacks.
Vishnu Hosur +2 more
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Gene stacking by recombinases [PDF]
Plant Biotechnology Journal, 2015 SummaryEfficient methods of stacking genes into plant genomes are needed to expedite transfer of multigenic traits to crop varieties of diverse ecosystems. Over two decades of research has identified several DNA recombinases that carryout efficient cis and trans recombination between the recombination sites artificially introduced into the plant ...
Vibha, Srivastava, James, Thomson
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Viruses, 2020 Bacteriophage 80α is a representative of a class of temperate phages that infect Staphylococcus aureus and other Gram-positive bacteria. Many of these phages carry genes encoding toxins and other virulence factors.
Keith A Manning, Terje Dokland
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RAG: a recombinase diversified [PDF]
Nature Immunology, 2009 During B cell and T cell development, the lymphoid-specific proteins RAG-1 and RAG-2 act together to initiate the assembly of antigen receptor genes through a series of site-specific somatic DNA rearrangements that are collectively called variable-diversity-joining (V(D)J) recombination.
Adam G W, Matthews +1 more
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eLife, 2021 While Cre-dependent viral systems permit the manipulation of many neuron types, some cell populations cannot be targeted by a single DNA recombinase.
Paul V Sabatini +7 more
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Biomolecules, 2020 Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to
Gaëlle Hogrel +7 more
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BRCA2: a universal recombinase regulator [PDF]
Oncogene, 2007 Homologous recombination has a dual role in eukaryotic organisms. Firstly, it is responsible for the creation of genetic variability during meiosis by directing the formation of reciprocal crossovers that result in random combinations of alleles and traits.
T, Thorslund, S C, West
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Modeling Gliomas Using Two Recombinases [PDF]
Cancer Research, 2019 Abstract Development of animal models to investigate the complex ecosystem of malignant gliomas using the Cre/loxP recombination system has significantly contributed to our understanding of the molecular underpinnings of this deadly disease.
Toshiro Hara, Inder M. Verma
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A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]
Shipin Kexue, 2023 With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
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Recombinase technology: applications and possibilities [PDF]
Plant Cell Reports, 2010 The use of recombinases for genomic engineering is no longer a new technology. In fact, this technology has entered its third decade since the initial discovery that recombinases function in heterologous systems (Sauer in Mol Cell Biol 7(6):2087-2096, 1987).
Wang, Yueju +3 more
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