Results 221 to 230 of about 99,506 (245)
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Cre Recombinase and Other Tyrosine Recombinases
Chemical Reviews, 2016Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the predictable modification of genomes as they enable precise genome editing in heterologous hosts. These enzymes are ubiquitous in eubacteria, prevalent in archaea and temperate phages, present in certain yeast strains, but barely found in higher eukaryotes.
Gretchen, Meinke +4 more
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Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
2017Recombinase-mediated cassette exchange, or RMCE, is a genome engineering tool that can be used to swap DNA fragments of interest between two DNA molecules. In a variation of RMCE, called dual RMCE, the exchange of DNA fragments is mediated by two recombinases in contrast to one recombinase in the classic RMCE reaction.
Eugenia, Voziyanova +2 more
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Safety Assessment of Cre Recombinase
Journal of Agricultural and Food Chemistry, 2006Cre recombinase, when used as a tool in agricultural biotechnology, can precisely excise DNA sequences that may be useful in the introduction of a new trait but are not needed in the commercial product. Although the cre genetic material would not be present in the final product, the present studies were performed to assess the safety of Cre recombinase
Ronald E, Hileman +4 more
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Catalysis by site-specific recombinases
Trends in Genetics, 1992Site-specific recombination reactions bring about controlled rearrangements of DNA molecules by cutting the DNA at precise points and rejoining the ends to new partners. The recombinases that catalyse these reactions can be grouped into two families by amino acid sequence homology.
W M, Stark, M R, Boocock, D J, Sherratt
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Conditional Gene Knockout Using Cre Recombinase
Molecular Biotechnology, 2001Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site.
Y, Le, B, Sauer
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Genome Engineering with Custom Recombinases
2014Site-specific recombinases are valuable tools for myriad basic research and genome engineering applications. In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications ...
Thomas, Gaj, Carlos F, Barbas
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Recombinase activating gene enzymes of lymphocytes
Current Opinion in Hematology, 2001Expression of T-cell receptor and surface immunoglobulins on T and B lymphocytes, respectively, is strictly dependent on the variable, (diversity) joining exon (V(D)J) recombination process, which is initiated by the lymphoid-specific recombinase activating gene proteins 1 and 2 (RAG1 and RAG2).
L D, Notarangelo, S, Santagata, A, Villa
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Phage Recombinases and Their Applications
2012The homologous recombination systems of linear double-stranded (ds)DNA bacteriophages are required for the generation of genetic diversity, the repair of dsDNA breaks, and the formation of concatemeric chromosomes, the immediate precursor to packaging.
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Strand Selection by the Tyrosine Recombinases
2005Publisher Summary This chapter focuses on four tyrosine recombinases for which there is a wealth of biochemical and structural data. This chapter reviews the biochemical and structural findings for each of these enzymes. This chapter also examines the factors that influence strand selection and consider the possible function of strand selection in ...
Linda, Lee, Paul D, Sadowski
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