Results 21 to 30 of about 99,506 (245)
Frontiers in Microbiology, 2017 Genomic analysis was performed on seven strains of Bifidobacterium thermacidophilum, a Sus-associated Bifidobacterium. Three strains from the feces of domestic pigs (Sus scrofa domesticus) and four strains from the rectal feces of free-range Japanese ...
Sayaka Tsuchida +6 more
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Monomer Networking Activates Recombinases [PDF]
Structure, 2006 Recombinases catalyze the DNA strand-exchange reactions of homologous recombination. The regularity of the filaments they form on DNA, though beautiful, does not explain how they work. Functionally important internal interactions now include multiple intermonomer contacts for activation (Galkin et al., 2006).
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Conditional Protein Splicing Switch in Hyperthermophiles through an Intein-Extein Partnership
mBio, 2018 Inteins are intervening proteins that undergo an autocatalytic splicing reaction that ligates flanking host protein sequences termed exteins. Some intein-containing proteins have evolved to couple splicing to environmental signals; this represents a new ...
Christopher W. Lennon +3 more
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Paleo-immunology: evidence consistent with insertion of a primordial herpes virus-like element in the origins of acquired immunity. [PDF]
PLoS ONE, 2009 BACKGROUND:The RAG encoded proteins, RAG-1 and RAG-2 regulate site-specific recombination events in somatic immune B- and T-lymphocytes to generate the acquired immune repertoire.
David H Dreyfus
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The RAG recombinase: Beyond breaking [PDF]
Mechanisms of Ageing and Development, 2017 DNA double-strand breaks (DSBs) are commonly seen as lesions that threaten genome integrity and contribute to cancer and aging processes. However, in the context of antigen receptor gene assembly, known as V(D)J recombination, DSBs are obligatory intermediates that allow the establishment of genetic diversity and adaptive immunity.
Lescale, Chloé, Deriano, Ludovic
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Next generation synthetic memory via intercepting recombinase function
Nature Communications, 2023 Here we present a technology to facilitate synthetic memory in a living system via repurposing Transcriptional Programming (i.e., our decision-making technology) parts, to regulate (intercept) recombinase function post-translation.
Andrew E. Short +3 more
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Frontiers in Oncology, 2014 We previously reported high expression of RAD51 and increased homologous recombination (HR) rates in multiple myeloma (MM) cells, and showed that genomic instability and disease progression are commensurate with HR levels. Moreover, high RAD51 expression
David A. Alagpulinsa +4 more
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Site‐specific recombinase genome engineering toolkit in maize
Plant Direct, 2020 Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, reactions result in
Jon P. Cody +4 more
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A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing
Nature Communications, 2022 Achieving spatial control of gene expression is important. Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre mouse line which they use for ...
Miguel Vizoso +7 more
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A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia
mSphere, 2019 Clostridia are a group of Gram-positive anaerobic bacteria of medical and industrial importance for which limited genetic methods are available. Here, we demonstrate an approach to make large genomic deletions and insertions in the model Clostridium ...
Tristan Cerisy +5 more
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