Results 31 to 40 of about 99,506 (245)

Prediction of designer-recombinases for DNA editing with generative deep learning

Nature Communications, 2022
Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholz   +3 more
doaj   +1 more source

The Possible Crystallization Process in the Origin of Bacteria, Archaea, Viruses, and Mobile Elements

Biology
We propose a hypothesis for the simultaneous emergence of bacteria, archaea, viruses, and mobile elements by sequential and concrete biochemical pathways. The emergence process can be considered analogous to crystallization, where genetic and biochemical
Akari Yoshimura, Masayuki Seki
doaj   +1 more source

A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

BMC Biology, 2022
Background The functional understanding of genetic interaction networks and cellular mechanisms governing health and disease requires the dissection, and multifaceted study, of discrete cell subtypes in developing and adult animal models.
Savannah J. Lusk, Andrew McKinney, Patrick J. Hunt, Paul G. Fahey, Jay Patel, Andersen Chang, Jenny J. Sun, Vena K. Martinez, Ping Jun Zhu, Jeremy R. Egbert, Genevera Allen, Xiaolong Jiang, Benjamin R. Arenkiel, Andreas S. Tolias, Mauro Costa-Mattioli, Russell S. Ray   +15 more
doaj   +1 more source

Cre Recombinase

Microbiology Spectrum, 2015
ABSTRACT The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in ...
openaire   +2 more sources

Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration

Scientific Reports, 2021
We previously reported efficient precision targeted integration of reporter DNA in zebrafish and human cells using CRISPR/Cas9 and short regions of homology.
Maira P. Almeida, Jordan M. Welker, Sahiba Siddiqui, Jon Luiken, Stephen C. Ekker, Karl J. Clark, Jeffrey J. Essner, Maura McGrail   +7 more
doaj   +1 more source

RUNX1 as a recombinase cofactor

Oncotarget, 2015
The regulation of TCR rearrangements, particularly of TCRδ and TCRβ, plays a decisive role in lymphoid differentiation and oncogenic transformation. Somatic assembly of TCR loci is established through V(D)J recombination during lymphocyte development.
Agata, Cieslak, Dominique, Payet-Bornet, Vahid, Asnafi   +2 more
openaire   +2 more sources

Establishment of a rapid method for the detection of Brucella canis based on recombinase-mediated thermostable nucleic acid amplification technology

Frontiers in Cellular and Infection Microbiology
ObjectiveTo establish a rapid detection method for canine brucellosis using recombinase-aided amplification (RAA) technology.MethodsThe outer membrane protein 25 gene fragment (Omp25) of Brucella canis was targeted.
Shao-Zheng Song, Zi-Yuan Li, Yuan-Yuan Liu, Ying-Chao Wu, Kang-Ying Yu, Zhengyi He   +5 more
doaj   +1 more source

The Serine Recombinases

Microbiology Spectrum, 2014
ABSTRACTIn site-specific recombination, two short DNA sequences (‘sites’) are each cut at specific points in both strands, and the cut ends are rejoined to new partners. The enzymes that mediate recognition of the sites and the subsequent cutting and rejoining steps are called recombinases.
openaire   +2 more sources

Diversity in the serine recombinases [PDF]

Molecular Microbiology, 2002
SummaryMost site‐specific recombinases fall into one of two families, based on evolutionary and mechanistic relatedness. These are the tyrosine recombinases orλintegrase family and the serine recombinases or resolvase/invertase family. The tyrosine recombinases are structurally diverse and functionally versatile and include integrases, resolvases ...
Margaret C M, Smith, Helena M, Thorpe
openaire   +2 more sources

Effects of p53 and ATRX inhibition on telomeric recombination in aging fibroblasts

Frontiers in Oncology
In order to avoid replicative senescence, tumor cells must acquire a telomere maintenance mechanism. Beside telomerase activation, a minority of tumors employs a recombinational mechanism called Alternative Lengthening of Telomeres (ALT). Several studies
Ion Udroiu, Jessica Marinaccio, Antonella Sgura   +2 more
doaj   +1 more source