Results 191 to 200 of about 520,105 (221)

Development of a recombinase-aided amplification assay for detection of orf virus

Journal of Virological Methods, 2020
Orf, caused by orf virus (ORFV), is an important zoonotic disease that infects goat and sheep, leading to huge economic losses. ORFV can also cause cutaneous lesions in people who come in close contact with the diseased animals. Although accurate diagnostic methods for ORFV infection exist, there is a need for a rapid, specific, and sensitive method ...
Wen Zhu, Li Yongdong, Yongqiu Cui
exaly   +3 more sources

Development of a recombinase-aided amplification assay for rapid and sensitive detection of porcine circovirus 3

Journal of Virological Methods, 2020
Porcine circovirus type 3 (PCV3) is a novel member of the genus Circovirus, first detected in the United States in 2016, with subsequent reports in many countries. PCV3 infections have caused serious economic losses in the pig industry. Alternative rapid and sensitive assays for PCV3 detection are needed for clinical diagnosis, especially in ...
Yong Wang, Li Yongdong, Hongxia Ni
exaly   +3 more sources

Sensitive detection of multiplex bacteria based on finger driven microfluidics and recombinase aided amplification

Biosensors and Bioelectronics
Foodborne pathogens, such as Salmonella typhimurium, E. coli O157:H7 and Listeria monocytogenes, have seriously threatened human health. To address the issues of traditional bacterial detection methods, such as long time, low sensitivity and complex operation, a versatile finger driven microfluidic chip was elaboratively developed and combined with ...
Jianhan Lin, Yanbin Li, Nana Jin
exaly   +3 more sources

Reverse transcription recombinase-aided amplification assay for avian influenza virus

Virus Genes, 2023
Avian influenza virus (AIV) infection can lead to severe economic losses in the poultry industry and causes a serious risk for humans. A rapid and simple test for suspected viral infection cases is crucial. In this study, a reverse transcription recombinase-aided amplification assay (RT-RAA) for the rapid detection of all AIV subtypes was developed ...
Suchun, Wang, Qingye, Zhuang, Nan, Jiang, Fuyou, Zhang, Qiong, Chen, Ran, Zhao, Yang, Li, Xiaohui, Yu, Jinping, Li, Guangyu, Hou, Liping, Yuan, Fuliang, Sun, Zihao, Pan, Kaicheng, Wang   +13 more
openaire   +2 more sources

Detection of pseudorabies virus with a real‐time recombinase‐aided amplification assay

Transboundary and Emerging Diseases, 2021
AbstractPseudorabies (PR) is an acute infectious disease of pigs caused by pseudorabies virus (PRV), which has caused great economic losses to the pig industry worldwide. Reliable and timely diagnose is crucial for the surveillance, control and eradication of PR.
Fei Tu, Yongning Zhang, Shengkui Xu, Xintan Yang, Lei Zhou, Xinna Ge, Jun Han, Xin Guo, Hanchun Yang   +8 more
openaire   +2 more sources

Real-time recombinase-aided amplification assay for rapid amplification of the IS1081 gene of Mycobacterium tuberculosis

European Journal of Clinical Microbiology & Infectious Diseases, 2023
Mycobacterium tuberculosis (MTB), the etiological agent of tuberculosis (TB), is the leading cause of death due to a single infectious agent worldwide. Rapid and accurate diagnosis of MTB is critical for controlling TB especially in resource-limited countries, since any diagnosis delay increases the chances of transmission.
Yuanyuan Liu, Weicong Ren, Zhongtan Xue, Yuedong Miao, Wei Wang, Xuxia Zhang, Cong Yao, Yuanyuan Shang, Shanshan Li, Fengling Mi, Yu Pang   +10 more
openaire   +2 more sources

Rapid detection of Salmonella with Recombinase Aided Amplification

Journal of Microbiological Methods, 2017
Rapid Salmonella detection using Recombinase Aided Amplification was established. The reaction completes in 20 min at 39°C and can be performed with a portable device. Once further improved, this method should be a great choice for monitoring contamination, such as foodborne Salmonella or for similar purposes.
Xiaoping, Zhang, Lichuan, Guo, Ranran, Ma, Lijuan, Cong, Zhonghua, Wu, Ying, Wei, Shijie, Xue, Wei, Zheng, Saijun, Tang   +8 more
openaire   +2 more sources

Development and evaluation of a sensitive recombinase aided amplification assay for rapid detection of Vibrio parahaemolyticus

Journal of Microbiological Methods, 2022
Vibrio parahaemolyticus (V. parahaemolyticus) is a widely distributed pathogen in the coastal areas, which causes food poisoning and leads to gastroenteritis and sepsis. Therefore, developing a simple, sensitive, and rapid detection method for V. parahaemolyticus is a major concern globally.
Zhi-Shan, Feng, Jing-Yi, Li, Jing-Yun, Zhang, Feng-Yu, Li, Hong-Xia, Guan, Rui-Qing, Zhang, Hong, Liu, Qi, Guo, Xin-Xin, Shen, Biao, Kan, Xue-Jun, Ma   +10 more
openaire   +2 more sources

Visual recombinase aided amplification technology for detecting feline coronavirus

The Veterinary Journal
Feline Coronavirus (FCoV) is responsible for causing intestinal lesions and potentially fatal systemic infections in cats, with a worldwide prevalence. Currently, there is no effective vaccine available, making early diagnosis essential for treatment. Although RT-PCR detection is known for its high specificity and accuracy, the method involves complex ...
Xiaoman Lu, Yilin Cao, Ping Zhang, Xiaoyuan Chen, David M. Irwin, Yongyi Shen   +5 more
openaire   +2 more sources

Development of an isothermal recombinase‐aided amplification assay for the rapid and visualized detection of Klebsiella pneumoniae

Journal of the Science of Food and Agriculture, 2022
AbstractBACKGROUNDKlebsiella pneumoniae is a zoonotic opportunistic pathogen, leading to severe infections in dairy cows and humans. Efficient, on‐site and accurate detection of K. pneumoniae is necessary to reduce the harm of cow mastitis and human infections.
Laiwang Hou, Darong Li, Ni Zhang, Jiayi Zhao, Yong Zhao, Xiaohong Sun   +5 more
openaire   +2 more sources