Results 201 to 210 of about 520,105 (221)

An automatic centrifugal system for rapid detection of bacteria based on immunomagnetic separation and recombinase aided amplification

Lab on a Chip, 2022
This study reported an automatic centrifugal system for rapid detection of foodborne pathogenic bacteria based on immunomagnetic separation (IMS) for target bacteria enrichment and recombinase aided amplification (RAA) for nucleic acid detection.
Siyuan Wang, Wuzhen Qi, Shangyi Wu, Jing Yuan, Hong Duan, Yanbin Li, Jianhan Lin   +6 more
openaire   +2 more sources

Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi

The American Journal of Tropical Medicine and Hygiene
ABSTRACT. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique that can amplify specific nucleic acids at a constant temperature (63–65°C) within a short period (<1 hour). In this study, we report the utilization of recombinase-aided LAMP to specifically amplify the 18S sRNA of Plasmodium knowlesi.
Meng Yee, Lai, Mohd Hafizi, Abdul Hamid, Jenarun, Jelip, Rose Nani, Mudin, Yee Ling, Lau   +4 more
openaire   +2 more sources

DNA Fragment Fusion and Nucleic Acid Detection by Fusion Recombinase-Aided Amplification

Analytical Chemistry
Constructing fusion DNA fragments is frequently used for genetic engineering purposes. To date, fusion PCR is one of the most popular approaches for generating fusion DNA fragments. Here, we describe a novel method for DNA fusion based on the isothermal DNA amplification technique, recombinase-aided amplification (RAA). We demonstrate that this method,
Xingxing Xiao, Xi Yang, Kexin Xu, Fuyuan Huang, Yan Zhang, Yelin Jiang, Yangbin Shi, Qinghong Zhou, Luying Wang, Jiahai Lu, Zongliang Gao, Yongliang Lou   +11 more
openaire   +2 more sources

Development of a Recombinase-Aided Amplification Assay for the Rapid Detection of Candida auris

Analytical Chemistry
Candida auris (C. auris) was first discovered in Japan in 2009 and has since spread worldwide. It exhibits strong transmission ability, high multidrug resistance, blood infectivity, and mortality rates. Traditional diagnostic techniques for C. auris have shortcomings, leading to difficulty in its timely diagnosis and identification.
Junxia Feng, Jinfeng Chen, Bing Du, Xiaohu Cui, Yuyan Xia, Guanhua Xue, Yanling Feng, Yuehua Ke, Hanqing Zhao, Jinghua Cui, Chao Yan, Lin Gan, Zheng Fan, Tongtong Fu, Ziying Xu, Yang Yang, Zihui Yu, Lijuan Huang, Shuo Zhao, Ziyan Tian, Zanbo Ding, Yujie Chen, Zhoufei Li, Jing Yuan   +23 more
openaire   +2 more sources

Sensitive Detection of Ralstonia solanacearum Using Recombinase-Aided Amplification and an Aerolysin Nanopore

Langmuir
Ralstonia solanacearum is a soil-borne plant pathogenic bacterium that causes bacterial wilt disease, leading to substantial economic losses in over 250 crops, including tomatoes, tobacco, and potatoes. We developed a novel method for the specific detection of R.
Bin Li, Xiaoqiang Wang, Dongmei Xi, Ying Wang   +3 more
openaire   +2 more sources

Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection

Diagnostic Microbiology and Infectious Disease, 2018
In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per ...
Chen, Chen, Xin-Na, Li, Gui-Xia, Li, Li, Zhao, Su-Xia, Duan, Teng-Fei, Yan, Zhi-Shan, Feng, Xue-Jun, Ma   +7 more
openaire   +2 more sources

Recombinase-Aided Amplification Combined with Lateral Flow Dipstick for the Rapid Detection of Pyrrhoderma noxium

Plant Disease
Pyrrhoderma noxium is an entry quarantine tree pathogenic fungus in China, which causes severe root rot and the death of many tree species, resulting in huge landscape, ecological, and economic losses. P. noxium is a soilborne plant pathogen, which makes it difficult to observe the symptoms of infected trees in the early stage.
Haiyue Yin, Haoyu Wu, Dianguang Xiong, Huayi Huang, Danyang Zhao, Chengming Tian   +5 more
openaire   +2 more sources

Recombinase aided amplification with photoreactive DNA-binding dye for rapid detection of viable

LWT - Food Science and Technology, 2021
Hengyi Xu, Xiaoyan Feng, Guoyang Xie
exaly  

Development and evaluation of a sensitive recombinase aided amplification assay for rapid detection of

Journal of Microbiological Methods, 2022
Biao Kan, Zhi-Shan Feng, Xin-Xin Shen
exaly  

The fluorescent probe-based recombinase-aided amplification for rapid detection of O157:H7

Molecular and Cellular Probes, 2021
Ju Liu, Hengyi Xu, Guoyang Xie
exaly