Results 151 to 160 of about 131,465 (263)
Droplet Digital CRISPR for Nucleic Acid Detection
Advanced Science, Volume 13, Issue 14, 9 March 2026.This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang +4 more
wiley +1 more source
O factor de trancrição NDT80 como regulator negativo da adesão e da formação de biofilme em Candida parapsilosis [PDF]
, 2014 Mestrado em Biologia Molecular e CelularC. parapsilosis infections incidence has been increasing for the past 20 years. Its caracteristics of adhering and forming biofilms are a critical factor for infection caused by this organism, affecting from ...
Gomes, Nuno Miguel Araújo da Cunha
core
MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection
Advanced Science, Volume 13, Issue 13, 3 March 2026.MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani +6 more
wiley +1 more source
Programming Next‐Generation Synthetic Biosensors by Genetic Circuit Design
Advanced Science, Volume 13, Issue 14, 9 March 2026.Synthetic biology enables genetic circuit‐based biosensing to detect diverse targets, process signals, and transduce them into readable outputs or intracellular regulatory activities. However, field deployment and real‐world application of such synthetic biosensors face considerable challenges in sensitivity, specificity, speed, stability, and ...
Yuanli Gao +4 more
wiley +1 more source
Biotechnology and Bioengineering, Volume 123, Issue 3, Page 776-784, March 2026.The probiotic E. coli Nissle 1917 was engineered to enable stable and antibiotic free protein expression platforms using native plasmids. Further modification aids in the production of high‐level protein expression for antimicrobial secretion whose activity is comparable to industry standard E. coli BL21.
Halimatun Sakdiah Zainuddin +2 more
wiley +1 more source
CRISPR Assays for Protein Detection
Chemistry–Methods, Volume 6, Issue 3, March 2026.This review summarizes recent advances in CRISPR–Cas–based strategies for protein detection. Particular focus is placed on recognition element–based approaches, in which antibodies, aptamers, or DNAzymes convert protein binding into CRISPR‐readable nucleic acid signals, and activity‐based strategies that exploit intrinsic protein functions to trigger ...
Luca Capelli +5 more
wiley +1 more source
Transposable elements: Functional aspects and applications as drivers of crop innovation
Crop Science, Volume 66, Issue 2, March/April 2026.Abstract Transposable elements (TEs), which were once relegated to the status of genomic parasites, are today accepted as important agents of plant genome evolution and adaptation. This review integrates evidence pointing to their dual function as drivers of genetic diversity and instability, with a focus on their contribution to phenotypic innovation,
Flávia Layse Belém Medeiros +12 more
wiley +1 more source
Shipin yu jixieObjectiveTo develop a novel fluorescence polarization (FP) detection strategy and achieve highly sensitive Salmonella detection by incorporating recombinase polymerase amplification (RPA) technology.MethodsThe detection mechanism relies on homologous ...
XUE Pengpeng +4 more
doaj +1 more source
Recognition, Signal Transduction, and Amplification of Nucleic Acids Under Isothermal Condition
Advanced Genetics, Volume 7, Issue 1, March 2026.This Perspective outlines a modular framework for isothermal nucleic acid detection, highlighting recognition and signal transduction as central determinants of assay performance, with amplification strategies serving as sensitivity enhancers. Emerging design principles and future opportunities for scalable and accessible diagnostics are discussed ...
Zhaohui Qin +3 more
wiley +1 more source
MedComm – Oncology, Volume 5, Issue 1, March 2026.Ageing acts as a double‐edged sword in cancer. In the elderly, open chromatin, immunosenescence, and chronic inflammation drive SASP (IL‐6, MMPs), MDSC accumulation and T‐cell suppression, fostering tumor‐promoting microenvironments and limited therapeutic benefit.
Qi Wang +7 more
wiley +1 more source