Results 161 to 170 of about 131,465 (263)

Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells [PDF]

open access: yes, 2008
Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy.
Jehonathan Pinthus   +2 more
core   +2 more sources

Environmental DNA‐based RPA‐CRISPR/Cas12a assay for on‐site detection of chironomid larvae in aquatic environments

open access: yesPest Management Science, Volume 82, Issue 3, Page 2458-2467, March 2026.
An environmental DNA (eDNA)‐based recombinase polymerase amplification (RPA)‐CRISPR/Cas12a assay enables rapid, specific, and on‐site detection of chironomids, supporting early warning, and ecological monitoring in freshwater environments. Abstract BACKGROUND Freshwater chironomid midges pose a significant threat to drinking water quality and ...
Kyuhyeong Kim   +5 more
wiley   +1 more source

Advances in Rapid Nucleic Acid Diagnostics for Hepatitis B and C Viruses: A Comprehensive Review

open access: yesReviews in Medical Virology, Volume 36, Issue 2, March 2026.
ABSTRACT Chronic viral hepatitis, predominantly caused by HBV and HCV infections, remains a major global health burden and a leading cause of cirrhosis, hepatocellular carcinoma (HCC), and liver‐related mortality. Achieving the World Health Organisation (WHO) target of diagnosing 90% of HBV and HCV infections by 2030 necessitates a fundamental ...
Hsin‐Ying Ho   +6 more
wiley   +1 more source

Transforming RNA Degradation into Accurate RNA Detection: RNase I–Assisted Rolling Circle Amplification for Clinical Samples

open access: yesAngewandte Chemie, Volume 138, Issue 9, 23 February 2026.
Here, we transform this otherwise destructive enzymatic activity into a powerful diagnostic advantage through an RNase I–assisted rolling circle amplification (RI‐RCA) strategy. By integrating controlled RNase I–mediated RNA digestion with circular DNA templates, this approach enables direct and highly sensitive detection of target RNA sequences. Using
Amal Mathai   +5 more
wiley   +2 more sources

Investigation of the substrate properties of fluorescently labeled pyrimidine triphosphates in recombinase polymerase amplification

open access: yesТонкие химические технологии
Objectives. To study the substrate properties of Cy5-labeled deoxynucleoside triphosphates of various natures (dU and dC) in the process of incorporation in the DNA chain during recombinase polymerase amplification (RPA).Methods.
A. S. Epifanov   +4 more
doaj   +1 more source

Loop‐Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review

open access: yesVeterinary Medicine and Science, Volume 12, Issue 2, March 2026.
Loop‐mediated isothermal amplification (LAMP) is a quick, cost‐effective molecular technique for detecting bacterial pathogens causing bovine mastitis. This review summarises 18 LAMP assays developed for key bacteria such as Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis and Escherichia coli.
Tewodros Fentahun Jember   +3 more
wiley   +1 more source

Deletion of Cardiac miR-17-92 Cluster Increases Ischemia/ Reperfusion Injury via PTEN Upregulation [PDF]

open access: yes, 2017
The miR-17- 92 cluster is necessary for cell proliferation and development of the cardiovascular system. Deletion of this cluster leads to death in neonatal mice.
Prakash, Meeta B
core   +1 more source

Techniques to Detect and Quantify the Bacterial Metalloprotease AprX in Bovine Milk: A Review

open access: yesComprehensive Reviews in Food Science and Food Safety, Volume 25, Issue 2, March 2026.
ABSTRACT The heat‐stable metalloprotease AprX, secreted by psychrotrophic Pseudomonas spp., is a major cause of quality deterioration in dairy products, particularly ultrahigh temperature (UHT) milk. This review synthesizes the evolution and current state of detection and quantification techniques for AprX in bovine milk, covering traditional ...
Aritra Sinha, Alan L. Kelly
wiley   +1 more source

A Scaffoldomics Platform for Modular In Vivo Enzyme Colocalisation and Its Application to Naringenin Biosynthesis

open access: yesMicrobial Biotechnology, Volume 19, Issue 3, March 2026.
A hierarchical assembly system was established in which the coding sequences for four docking enzymes and a scaffold are co‐assembled onto a single plasmid, thereby establishing a ‘scaffoldomics’ platform for the optimisation of multi‐enzyme biosynthetic pathways.
Marte Elias   +5 more
wiley   +1 more source

Silencing of the Metabolic Gene HKDC1 Is Associated With Aging and Neurodegeneration in Mice and Humans

open access: yesAging Cell, Volume 25, Issue 3, March 2026.
Model showing decline in HKDC1 gene expression with aging. In a young cell, TFEB gets recruited to its binding sites on the HKDC1 promoter to upregulate HKDC1 expression, which interacts with mitochondria and stabilizes PINK1 to maintain mitochondrial health.
Zeenat Farooq   +8 more
wiley   +1 more source

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