Results 91 to 100 of about 17,679 (284)
Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. [PDF]
PLoS ONE, 2010 Feasibility of chromosomal manipulation in mammalian cells was first reported 15 years ago. Although this technique is useful for precise understanding of gene regulation in the chromosomal context, a limited number of laboratories have used it in actual
Munehiro Uemura +4 more
doaj +1 more source
Tangle Solutions for a Family of DNA-Rearranging Proteins
, 2004 We study two systems of tangle equations that arise when modeling the action of the Integrase family of proteins on DNA. These two systems--direct and inverted repeats--correspond to two different possibilities for the initial DNA sequence.
Buck, Dorothy +1 more
core +1 more source
Integrative and Conjugative Elements (ICEs): What They Do and How They Work [PDF]
, 2015 Horizontal gene transfer plays a major role in microbial evolution, allowing microbes to acquire new genes and phenotypes. Integrative and conjugative elements (ICEs, a.k.a.
Grossman, Alan D. +3 more
core +1 more source
Advanced Science, EarlyView.Lipid overload suppresses SREBF2‐mediated FNTB expression, leading to defective Lamin A maturation and nuclear envelope instability. This nuclear catastrophe triggers a pro‐fibrotic senescence program in cardiomyocytes. Notably, restoring nuclear integrity via AAV9‐based gene therapy effectively attenuates cardiac remodeling, identifying the ...
Yuxiao Chen +16 more
wiley +1 more source
Genome engineering in Bacillus anthracis using tyrosine site-specific recombinases. [PDF]
PLoS ONE, 2017 Tyrosine site-specific recombinases (T-SSR) are polynucleotidyltransferases that catalyze cutting and joining reactions between short specific DNA sequences. We developed three systems for performing genetic modifications in Bacillus anthracis that use T-
Andrei P Pomerantsev +5 more
doaj +1 more source
Rational tangle surgery and Xer recombination on catenanes
, 2011 The protein recombinase can change the knot type of circular DNA. The action of a recombinase converting one knot into another knot is normally mathematically modeled by band surgery.
Conway +5 more
core +1 more source
Cleavage‐Resistant CYLD Protects Against Autoimmune Hepatitis
Advanced Science, EarlyView.Proteolytic cleavage of the deubiquitinase CYLD emerges as a critical driver of autoimmune hepatitis. TNFα‐induced CYLD loss in macrophages amplifies S100A9‐triggered MAPK activation, leading to excessive chemokine production and hepatic inflammation. Pharmacological inhibition of MEK signaling effectively attenuates experimental disease, highlighting ...
Han Liu +13 more
wiley +1 more source
Hepatology, EarlyView., 2022 Abstract Background and Aims Intrahepatic cholangiocarcinoma (ICC) is a deadly but poorly understood disease, and its treatment options are very limited. The aim of this study was to identify the molecular drivers of ICC and search for therapeutic targets.
Yuto Shiode +16 more
wiley +1 more source
The site-specific integration reaction of
Mobile DNA, 2013 Background A large subfamily of serine recombinases contains long polypeptide segments appended to the C-terminal end of the conserved catalytic domain.
Mandali Sridhar +4 more
doaj +1 more source
MEIOB targets single-strand DNA and is necessary for meiotic recombination. [PDF]
PLoS Genetics, 2013 Meiotic recombination is a mandatory process for sexual reproduction. We identified a protein specifically implicated in meiotic homologous recombination that we named: meiosis specific with OB domain (MEIOB).
Benoit Souquet +12 more
doaj +1 more source