Results 111 to 120 of about 10,769 (161)

Diversity and abundance of antimicrobial resistance genes in manure from pig farms with varying antibiotic use: a long-read metagenomic sequencing approach. [PDF]

Porcine Health Manag
Biggel M, Oberhänsli T, Kümmerlen D, Walkenhorst M, Stephan R, Holinger M.   +5 more
europepmc   +1 more source

Efficient high-precision transgene knock-in by Recombinases (Redα/β)-enhanced DNA integration-CRISPR-Cas9 (RED-CRISPR). [PDF]

Nat Commun
Li W, Liu S, Fang X, Zou J, Jiang Q, Min X, Zhu X, Cao Y, Gao X, Han W, Azhar M, Xing X, Li F, Zhang Y, Liu H, Cheng L, Wang C, Bao J.   +17 more
europepmc   +1 more source

Prophage φEr670 and Genomic Island GI_Er147 as Carriers of Resistance Genes in <i>Erysipelothrix rhusiopathiae</i> Strains. [PDF]

Int J Mol Sci
Dec M, Zomer AL, Broekhuizen-Stins MJ, Urban-Chmiel R.   +3 more
europepmc   +1 more source

Characterization of recombinase-based genetic parts and circuits using nanopore sequencing

Greco FV, Cameron SK, Joshi SH, Guiziou S, Brophy JAN, Grierson CS, Gorochowski TE.   +6 more
europepmc   +1 more source

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Cre Recombinase and Other Tyrosine Recombinases

Chemical Reviews, 2016
Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the predictable modification of genomes as they enable precise genome editing in heterologous hosts. These enzymes are ubiquitous in eubacteria, prevalent in archaea and temperate phages, present in certain yeast strains, but barely found in higher eukaryotes.
Gretchen, Meinke, Andrew, Bohm, Joachim, Hauber, M Teresa, Pisabarro, Frank, Buchholz   +4 more
openaire   +2 more sources

Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases

Methods in Molecular Biology, 2017
Recombinase-mediated cassette exchange, or RMCE, is a genome engineering tool that can be used to swap DNA fragments of interest between two DNA molecules. In a variation of RMCE, called dual RMCE, the exchange of DNA fragments is mediated by two recombinases in contrast to one recombinase in the classic RMCE reaction.
Eugenia, Voziyanova, Rachelle P, Anderson, Yuri, Voziyanov   +2 more
exaly   +3 more sources

Catalysis by site-specific recombinases

Trends in Genetics, 1992
Site-specific recombination reactions bring about controlled rearrangements of DNA molecules by cutting the DNA at precise points and rejoining the ends to new partners. The recombinases that catalyse these reactions can be grouped into two families by amino acid sequence homology.
W M, Stark, M R, Boocock, D J, Sherratt
openaire   +2 more sources

Phage Recombinases and Their Applications

2012
The homologous recombination systems of linear double-stranded (ds)DNA bacteriophages are required for the generation of genetic diversity, the repair of dsDNA breaks, and the formation of concatemeric chromosomes, the immediate precursor to packaging.
openaire   +2 more sources