Results 141 to 150 of about 12,788 (278)

Small serine recombinases are markers for antiphage defense system discovery. [PDF]

PLoS Biol
Andersen SE, Kirsch JM, Singh N, Garrett SR, Whitney JC, Hesselberth JR, Duerkop BA.   +6 more
europepmc   +1 more source

Mobile genetic elements shape microbial diversity and functions in thawing permafrost soils. [PDF]

Nat Microbiol
Guo J, Aroney STN, Domínguez-Huerta G, Smith D, Vik D, Owusu-Ansah C, Pratama AA, Solonenko S, Tian F, Howard-Varona C, Zhong ZP, Fofana A, Smith GJ, Hodgkins SB, Cronin D, IsoGenie Field Teams 2010–2017, 2019, EMERGE Coordinators, Woodcroft BJ, Tyson GW, Rich VI, Sullivan MB, Roux S, Bagby SC.   +23 more
europepmc   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Large serine recombinase-mediated gene insertion for high-throughput screens: advantages, design principles, and applications. [PDF]

Nucleic Acids Res
Martella A, Matreyek KA, Fisher DI.
europepmc   +1 more source

A Novel Sparse Cellular Labeling System with Tunable Gradients and Long-Term Stability. [PDF]

Adv Sci (Weinh)
Tang X, Hou Z, Lu L, Song Y, Chai C, Li L, Shen Y, Wang S, Zhao Y, Zeng J, Guo Y, Jiang F, Xiang Z, Li H, He A, Zhang B, Lu Y, Li X.   +17 more
europepmc   +1 more source

Rapid Detection of Klebsiella pneumoniae in Infants With Severe Infection Disease by Instant Nanofluorescent Microspheres‐Immunochromatography Combined With Recombinase Polymerase Isothermal Amplification Technology

Journal of Clinical Laboratory Analysis, EarlyView.
Schematic illustration of the RPA‐FICTS platform for rapid detection of Klebsiella pneumoniae in infants. Genomic DNA extracted from clinical samples is amplified by recombinase polymerase amplification (RPA) targeting the mdh gene at 37°C, and the biotin‐ and digoxigenin‐labeled amplicons are subsequently detected on a nanofluorescent microsphere ...
Yiping Liu, Yi Lu, Xin Wang, Hanzhong Yu, Yumeng Lu, Minghui Chen, Feifan Zhu, Xing Lv, Li Wang, Ying Chen   +9 more
wiley   +1 more source

Mechanisms That Govern Recombinase Fidelity Control During Eukaryotic Homologous Recombination. [PDF]

Bioessays
Korkmaz I, Crickard JB.
europepmc   +1 more source

Low‐Concentration Enhancement Effect: Enabling Sensitive Detection of EML4‐ALK Variants via Multiplex RT‐MIRA

Journal of Clinical Laboratory Analysis, EarlyView.
This study developed an optimized multiplex RT‐MIRA assay for the simultaneous detection of EML4‐ALK variants, achieving single‐copy sensitivity. Unexpectedly, during the development of the reaction system, we discovered a “low‐concentration enhancement effect,” where reducing primer or probe concentrations within a specific range markedly improved ...
Xiao‐Dong Ren, Xi‐Yao Luo, Jin‐Mi Li, Xian‐Ge Sun, Jing Lv, Hua‐Liang Xiao, Qiu‐Shi Wang, Hong Wang, Chun‐Yan Wang, Hao‐Zheng Li, Qing Huang   +10 more
wiley   +1 more source

Programmable RNA-Guided DNA Recombination: Mechanisms, Engineering, and Applications. [PDF]

Biomedicines
Ali Agha ASA, Hattab D, Bakhtiar A, Al Khatib AO, Abushahla HS, Alketbi S, Akour A.   +6 more
europepmc   +1 more source

Anticancer potential of berberine: Molecular pathways and current clinical trial perspectives

JSFA reports, EarlyView.
Abstract Cancer is constantly rising mortality rates due to its late prognosis, poor management, and expensive treatment. Multi‐sectoral approaches for cancer management include hygienic practices, synthetic drug exploitation, radiation therapy, and diet modifications.
Muhammad Maaz, Muhammad Tauseef Sultan, Muhammad Israr, Waqas Ahmad Khan, Fahad Tanveer, Muzzamal Hussain, Muhammad Imran, Ahmed Mujtaba, Anjuman Gul Memon, Muhammad Atif, Entessar Al Jbawi   +10 more
wiley   +1 more source