Results 151 to 160 of about 17,679 (284)

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Nature Communications
Site-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the CRISPR-Cas toolbox and provide the additional benefit of high-efficiency DNA ...
Charlotte Cautereels, Jolien Smets, Jonas De Saeger, Lloyd Cool, Yanmei Zhu, Anna Zimmermann, Jan Steensels, Anton Gorkovskiy, Thomas B. Jacobs, Kevin J. Verstrepen   +9 more
doaj   +1 more source

Whole tissue imaging of cellular boundaries at sub‐micron resolutions for deep learning cell segmentation: Applications in the analysis of epithelial bending of ectoderm

Developmental Dynamics, EarlyView.
Abstract Background To understand cellular morphology, biologists have relied on traditional optical microscopy of tissues combined with tissue clearing protocols to image structures deep within tissues. Unfortunately, these protocols often struggle to retain cell boundary markers, especially at high enough resolutions necessary for precise cell ...
Sam C. P. Norris, Jimmy K. Hu, Neil H. Shubin   +2 more
wiley   +1 more source

Heterozygous Med13l mice recapitulate a developmental growth delay and craniofacial anomalies seen in MED13L syndrome

Developmental Dynamics, EarlyView.
Abstract Background Gene transcription is crucial for embryo and postnatal development and is regulated by the Mediator complex. Mediator is comprised of four submodules, including the kinase submodule (CKM). The CKM consists of MED13, MED12, CDK8, and CCNC.
Anna K. Leinheiser, Timothy T. Nguyen, Kayla M. Henry, Mariela Rosales, Eric Van Otterloo, Chad E. Grueter   +5 more
wiley   +1 more source

Germline Transgenic Methods for Tracking Cells and Testing Gene Function During Regeneration in the Axolotl

, 2013
The salamander is the only tetrapod that regenerates complex body structures throughout life. Deciphering the underlying molecular processes of regeneration is fundamental for regenerative medicine and developmental biology, but the model organism had ...
Khattak, Shahryar, Schuez, Maritta, Richter, Tobias, Knapp, Dunja, Haigo, Saori L., Sandoval-Guzmán, Tatiana, Hradlikova, Kristyna, Duemmler, Annett, Kerney, Ryan R., Tanaka, Elly M.   +9 more
core   +3 more sources

Expression of mutant TIE2 p.L914F during mouse development causes embryonic lethality and defects in vascular remodeling

Developmental Dynamics, EarlyView.
Abstract Background Sporadic venous malformation (VM) is associated with the hyperactivating p.L914F mutation in TIE2, a receptor tyrosine kinase essential for vascular development. This mutation is not found in hereditary VM, suggesting incompatibility with life when expressed during early vascular development.
Lindsay J. Bischoff, Sandra Schrenk, Kara Soroko, Chhiring Sherpa, Ashok Arasu, Damien Reynaud, Elisa Boscolo   +6 more
wiley   +1 more source

Long‐lasting remodeling of astrocytes in an Scna1+/− mouse model of Dravet syndrome

Epilepsia, EarlyView.
Abstract Objective Dravet syndrome (DS) is a prototypical developmental and epileptic encephalopathy caused by mutations in the SCN1A gene, leading to loss of function of the voltage‐gated sodium channel Naᵥ1.1. The latter causes early onset drug‐resistant seizures and enduring cognitive and behavioral deficits.
Athénaïs Genin, Alicia Janvier, Tristan Moujellil‐Legagneur, Marine Blaquière, Alexis Chaussy, Romane Privé, Fabrice Duprat, Massimo Mantegazza, Etienne Audinat, Nicola Marchi, Noémie Cresto   +10 more
wiley   +1 more source

Phage parasites targeting phage homologous recombinases provide antiviral immunity

Nature Communications
Bacteria often carry multiple genes encoding anti-phage defense systems, clustered in defense islands and phage satellites. Various unrelated anti-phage defense systems target phage-encoded homologous recombinases (HRs) through unclear mechanisms.
Gianluca Debiasi-Anders, Cuncun Qiao, Amrita Salim, Na Li, Ignacio Mir-Sanchis   +4 more
doaj   +1 more source

Spherical Nucleic Acid Stabilized Cage Type Three‐Dimensional Electrochemiluminescence Probe for Sensitive and Amplification‐Free Diagnosis of Infectious Diseases

Exploration, EarlyView.
This work developed a spherical nucleic acid stabilized cage‐type 3D electrochemiluminescence reporter probe for amplification‐free and ultrasensitive detection of pathogenic. Furthermore, a hand‐held detection device was designed, and we established a straightforward workflow enabling sample to answer within 15 min.
Yu Fu, Wenlu Song, Jinyan Lin, Wei Wang, Lunjing Liu, Wenjie Wu, Huiyi Yang, Jingru Wang, Yang Yang, Bowen Shu, Yideng Jiang, Yuhui Liao   +11 more
wiley   +1 more source

Guidelines for Light‐Start One‐Pot RPA‐CRISPR‐Cas12a Assays for Foodborne Pathogen Detection

Food Safety and Health, EarlyView.
Light‐start one‐pot RPA‐CRISPR‐Cas12a detection schematic diagram. (A) Linear crRNA activation light‐start one‐pot workflow. (B) Caged crRNA activation light‐start one‐pot workflow. ABSTRACT Rapid and accurate detection of nucleic acids of foodborne pathogens is of vital importance for controlling the spread of foodborne diseases and safeguarding ...
Fan Li, Ruiting Yao, Xinxin Jiang, Haorui Zhu, Guoliang Li   +4 more
wiley   +1 more source