Results 181 to 190 of about 12,788 (278)

Design and in silico validation of donor DNA for RNA-guided recombinase-mediated knockout of mstnb gene in Labeo rohita. [PDF]

PLoS One
Singh LS, Murugesan P, Iqbal G, Nidarshan NC, Gunasekaran H, Viralkumar A, Rasal KD, Brahmane MP, Nayak SK, Sonwane AA, Goswami M.   +10 more
europepmc   +1 more source

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source

Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by an <i>Acinetobacter baylyi</i> ADP-ISx antibiotic gene biosensor strain. [PDF]

MicroPubl Biol
Rhyan N, Koshy IS, Dinh R, VanDieren AJ, Mishler DM, Barrick JE.   +5 more
europepmc   +1 more source

The trimeric Shu complex in C. elegans is an ATPase that remodels RAD51 filaments in the homologous recombination‐associated DNA damage response

The FEBS Journal, EarlyView.
The C. elegans Shu complex (RFS‐1/RIP‐1/SWS‐1) is a DNA‐dependent ATPase that regulates RAD‐51 filaments during homologous recombination. The trimer preferentially binds 5′‐exposed DNA, remodels RAD‐51 filaments via ATP hydrolysis, and stabilizes filaments through ATP binding.
Sam Siu Hang Chu, Guangxin Xing, Kichang Paik, Anton Gartner, Hong Ling   +4 more
wiley   +1 more source

Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases. [PDF]

J Biol Chem
Petassi M, Shin Y, Jessop AM, Morse K, Kim SY, Kuppa S, Matei R, Raina VB, Greene EC.   +8 more
europepmc   +1 more source

From biology to biotechnology: Host‐regulation factors from parasitoid wasps are a source of bioactive molecules with translational potential

Insect Molecular Biology, EarlyView.
Parasitoid wasps deploy maternal and embryonic factors to reprogramme host physiology. Venom, calyx fluid, polydnaviruses, teratocytes and larval secretions act in a coordinated, compartmentalised manner. Host‐regulation factors are promising sources of insecticidal, antimicrobial and bioinspired translational molecules.
Ciro Pedro G. Pinto, Andrew A. Walker, Glenn F. King   +2 more
wiley   +1 more source

Bxb1 and ParA recombinase-based gene assembly for multiple genes expression in plant cells. [PDF]

Plant Physiol
Meng X, Wang S, Huang D, Wan H, Chen Y, Liu D, Chen S, Tan G, Cao X, Sun W, Chen W.   +10 more
europepmc   +1 more source

A light-dependent Flp recombinase [PDF]

Nature Methods, 2019
openaire   +2 more sources

A CRISPR‐Based Rapid Detection Assay for Crayfish Plague (Aphanomyces astaci) From Environmental Samples

Journal of Fish Diseases, EarlyView.
ABSTRACT Crayfish plague, caused by Aphanomyces astaci (Aa), is an infectious disease invasive in Europe, where its rapid spread has resulted in sharp declines of native crayfish species. Monitoring currently relies on a highly sensitive, but costly and time‐consuming qPCR approach.
Christopher Heine, Amadeus Plewnia, David Strand, Simran Gupta, Henrik Krehenwinkel, Gus R. McFarlane   +5 more
wiley   +1 more source

Control of VDJ Recombinase Activity

Cold Spring Harbor Symposia on Quantitative Biology, 1989
P. Ferrier, B. Krippl, A.J. Furley, T.K. Blackwell, H. Suh, M. Mendelsohn, A. Winoto, W.D. Cook, L. Hood, F. Costantini, F.W. Alt   +10 more
openaire   +2 more sources