Results 191 to 200 of about 12,788 (278)

Development of a Multiplex Polymerase Chain Reaction Assay for Differentiating Three Lactococcus Species Associated With Piscine Lactococcosis

Journal of Fish Diseases, EarlyView.
ABSTRACT Piscine lactococcosis is an important bacterial disease of farmed fish. The causative agents, Lactococcus garvieae, Lactococcus petauri and Lactococcus formosensis, are closely related, which complicates species‐level identification. We developed a conventional multiplex PCR assay targeting species‐specific genes identified by comparative ...
Tomoaki Yoshino, Tomokazu Takano, Hiroki Tanno, Yukihiro Nakagawa, Yuta Matsuura, Shogo Harakawa, Hidehiro Kondo, Ikuo Hirono, Keiichiro Koiwai, Tomomasa Matsuyama   +9 more
wiley   +1 more source

Latent endogenous giant viruses drive active infection and inheritance in a multicellular algal host. [PDF]

Nat Microbiol
Duchêne C, Craig RJ, Martinho C, Luthringer R, Agullo F, Hipp K, Escudeiro P, Alva V, Haas FB, Coelho SM.   +9 more
europepmc   +1 more source

ATXR5 and ATXR6 restrict meiotic crossover formation within heterochromatin in Arabidopsis

Journal of Integrative Plant Biology, EarlyView.
The Arabidopsis H3K27 mono‐methyltransferases ATXR5 and ATXR6 repress crossovers within meiotic heterochromatin, are required for heterochromatin condensation and H3K27me1 establishment, and specifically influence the maintenance of DNA methylation in meiocytes.
Jun Zhang, Yue Yu, Han Wang, Meiling Li, Dingning Li, Chenjiang You, Yingxiang Wang, Cong Wang   +7 more
wiley   +1 more source

Advances in large-scale DNA engineering with the CRISPR system. [PDF]

Exp Mol Med
Gwon LW, Badon IW, Lee Y, Kim HJ, Lee SH.   +4 more
europepmc   +1 more source

Chronic silencing of subsets of cortical layer 5 pyramidal neurons has a long‐term influence on the laminar distribution of parvalbumin interneurons and the perineuronal nets

Journal of Anatomy, Volume 246, Issue 4, Page 479-504, April 2025.
Chronic silencing of subsets of layer 5 projection neurons has distinct effects on the laminar distribution of PV interneurons and the perineuronal nets in the adult primary motor and somatosensory cortex. Abstract Neural networks are established throughout cortical development, which require the right ratios of glutamatergic and GABAergic neurons ...
Florina P. Szabó, Veronika Sigutova, Anna M. Schneider, Anna Hoerder‐Suabedissen, Zoltán Molnár   +4 more
wiley   +1 more source

From Negative Feedback to Integrated Control: Recombinase-Based Strategies for Mitigating Resource Competition. [PDF]

Methods Mol Biol
Zhang R, Zhang R, Tian XJ.
europepmc   +1 more source

A Lambda Red Recombinase‐Assisted CRISPR/Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciens

Plant Biotechnology Journal, EarlyView.
ABSTRACT Agrobacterium tumefaciens is a powerful delivery tool for plant genetic transformation and a pivotal platform for agricultural biotechnology. However, laboratory strains of Agrobacterium have remained genetically unoptimized for decades, with genome engineering hindered by inefficient, endogenous homologous recombination systems.
Minmin Zheng, Rui Liu, Wei‐Chang Huang, Zuren Li, Kai Hua   +4 more
wiley   +1 more source

Optimization of IS621 recombinase/bridge RNA-directed recombination for precise insertion of large DNA fragments in human cells. [PDF]

Nat Commun
Guo J, Song Z, Wang W, Huang S, Zhang Y, Li G, Huang X, Wang X, Liu J, Wu L.   +9 more
europepmc   +1 more source

Adenine nucleotide translocator and ATP synthase cooperate in mediating the mitochondrial permeability transition

The Journal of Physiology, EarlyView.
Abstract figure legend Both ATP synthase and adenine nucleotide translocator (ANT) play key roles in mediating the permeability transition. The contribution of ANT becomes evident in the presence of atractylate (ATR) at pH 6.5, a condition that prevents ATP synthase channel activity. At pH 7.4, this effect is minimal, suggesting a critical role for ATP
Ludovica Tommasin, Andrea Carrer, Federica Boscolo Nata, Elena Frigo, Federico Fogolari, Giovanna Lippe, Michela Carraro, Paolo Bernardi   +7 more
wiley   +1 more source

Development of efficient genetic toolkits and heterologous chassis for bioprospecting of myxobacteria. [PDF]

Nat Commun
Zhang WJ, Hu JQ, Li RJ, Sheng DH, Yue XJ, She Q, Zhang Y, Li YZ, Zhuo L, Wu C.   +9 more
europepmc   +1 more source